Phenotypes associated with this allele

• Allele Symbol: Tg(H2-Ea-Ins2)1Wehi
• Allele Name: transgene insertion 1, William and Eliza Hall Institute
• Allele ID: MGI:3590216
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(H2-Ea-Ins2)1Wehi/Tg(H2-Ea-Ins2)1Wehi	NOD/ShiLtJWehi-Tg(H2-Ea-Ins2)1Wehi	MGI:3790762
tg2	Tg(H2-Ea-Ins2)1Wehi/0	NOD/ShiLtJWehi-Tg(H2-Ea-Ins2)1Wehi	MGI:3590228

Genotype
MGI:3790762

tg1

• Allelic Composition: Tg(H2-Ea-Ins2)1Wehi/Tg(H2-Ea-Ins2)1Wehi
• Genetic Background: NOD/ShiLtJWehi-Tg(H2-Ea-Ins2)1Wehi

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:117352 )

N • G6pc2_206-214-specific T cells have cytotoxic potential in vitro but are 'ignorant' of their antigen

N • dendritic cell antigen presentation to dendritic cells from wild-type NOD mice; antigen presenting cell function is not affected

decreased T cell proliferation ( J:117352 )

• G6pc2_206-214-specific CD8+ T cells transferred from Tg(TcraTcrbNY8.3)1Pesa NOD mice proliferate with much lower efficiency compared to cells transferred to wild-type NOD hosts

• no regulatory T cell generation is indicated by thymic expression of autoantigens

abnormal CD8-positive, alpha beta T cell morphology ( J:117352 )

• T cells specific for IGRP_206-214 (G6pc2_206-214) are almost undetectable in absence of priming in peripheral blood or lymphoid tissues in NOD mice expressing the transgene, at any age assayed, while such cells are readily detected and peak at 15 weeks of age in wild-type NOD controls

decreased anti-insulin autoantibody level ( J:117352 )

• mice do not show an insulin autoantibody response, compared to wild-type NOD controls

hematopoietic system

hematopoietic system phenotype ( J:117352 )

N

decreased T cell proliferation ( J:117352 )

• G6pc2_206-214-specific CD8+ T cells transferred from Tg(TcraTcrbNY8.3)1Pesa NOD mice proliferate with much lower efficiency compared to cells transferred to wild-type NOD hosts

• no regulatory T cell generation is indicated by thymic expression of autoantigens

abnormal CD8-positive, alpha beta T cell morphology ( J:117352 )

• T cells specific for IGRP_206-214 (G6pc2_206-214) are almost undetectable in absence of priming in peripheral blood or lymphoid tissues in NOD mice expressing the transgene, at any age assayed, while such cells are readily detected and peak at 15 weeks of age in wild-type NOD controls

cellular

decreased T cell proliferation ( J:117352 )

• G6pc2_206-214-specific CD8+ T cells transferred from Tg(TcraTcrbNY8.3)1Pesa NOD mice proliferate with much lower efficiency compared to cells transferred to wild-type NOD hosts

• no regulatory T cell generation is indicated by thymic expression of autoantigens

Genotype
MGI:3590228

tg2

• Allelic Composition: Tg(H2-Ea-Ins2)1Wehi/0
• Genetic Background: NOD/ShiLtJWehi-Tg(H2-Ea-Ins2)1Wehi

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:100232 )

N • unlike wild-type NOD mice, transgenic NOD/LtWehi mice do not develop spontaneous diabetes

N • whereas wildtype NOD mice are susceptible to diabetes induction by cyclophosphamide, no transgenic NOD/LtWehi mice became diabetic (defined as blood glucose > 11 mmol/l) after a single cyclophosphamide injection; following a second injection only one of ten developed diabetes, and it was more slowly progressive than in wild-type controls

N • pancreata of transgenic NOD/LtWehi mice are almost entirely free of insulitis: 90% of 244 islets from 100-day-old female mice and 98% of 259 islets from 147-day-old males had no cellular infiltration, and the rest exhibited only a minimal degree of insulitis

salivary gland inflammation ( J:100232 )

• the transgene does not affect the characteristic susceptibility of NOD mice to sialitis, the degree of which does not differ significantly between transgenic NOD/LtWis mice and control littermates

immune system

immune system phenotype ( J:100232 )

N • pancreata of transgenic NOD/LtWehi mice are almost entirely free of insulitis: 90% of islets from 100-day-old female mice and 98% of those from 147-day-old males have no cellular infiltration, and the rest exhibit only a minimal degree of insulitis

abnormal T cell proliferation ( J:100232 )

• spleen- and draining lymph node-derived T cells from wild-type and transgenic NOD/LtWehi mice exhibit statistically similar in vitro proliferative responses to peptides derived from proinsulin and GAD2 (GAD65) following immunization with these self antigens

salivary gland inflammation ( J:100232 )

• the transgene does not affect the characteristic susceptibility of NOD mice to sialitis, the degree of which does not differ significantly between transgenic NOD/LtWis mice and control littermates

digestive/alimentary system

salivary gland inflammation ( J:100232 )

• the transgene does not affect the characteristic susceptibility of NOD mice to sialitis, the degree of which does not differ significantly between transgenic NOD/LtWis mice and control littermates

hematopoietic system

abnormal T cell proliferation ( J:100232 )

• spleen- and draining lymph node-derived T cells from wild-type and transgenic NOD/LtWehi mice exhibit statistically similar in vitro proliferative responses to peptides derived from proinsulin and GAD2 (GAD65) following immunization with these self antigens

cellular

abnormal T cell proliferation ( J:100232 )

• spleen- and draining lymph node-derived T cells from wild-type and transgenic NOD/LtWehi mice exhibit statistically similar in vitro proliferative responses to peptides derived from proinsulin and GAD2 (GAD65) following immunization with these self antigens

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
type 1 diabetes mellitus		DOID:9744	OMIM:222100	J:100251