Phenotypes associated with this allele

• Allele Symbol: Tg(Col1a1-cre)1Ack
• Allele Name: transgene insertion 1, Andrew C Karaplis
• Allele ID: MGI:3603124
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Pthlh^tm1Ack/Pthlh^tm1Ack Tg(Col1a1-cre)1Ack/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N	MGI:3617818
cn2	Gt(ROSA)26Sor^tm4(Wnt7b)Flng/Gt(ROSA)26Sor^+ Tg(Col1a1-cre)1Ack/0	involves: FVB/N	MGI:5613584
cn3	Gt(ROSA)26Sor^tm5(Wnt5a)Flng/Gt(ROSA)26Sor^tm5(Wnt5a)Flng Tg(Col1a1-cre)1Ack/0	involves: FVB/N	MGI:5613587

Genotype
MGI:3617818

cn1

• Allelic Composition: Pthlh^tm1Ack/Pthlh^tm1Ack; Tg(Col1a1-cre)1Ack/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

abnormal osteoclast morphology ( J:100918 )

• the number and surface of osteoclasts is reduced by 43% and 52%, respectively

decreased osteoclast cell number ( J:100918 )

decreased bone mineral density ( J:100918 )

• long bones appear less radiopaque and bone mineral density is decreased

abnormal osteoblast morphology ( J:100918 )

• osteoblast number and surface are decreased by 55% and 60%, respectively and osteoid volume is decreased by 75%

decreased osteoblast cell number ( J:100918 )

• the percentage of apoptotic osteoblasts/osteocytes is increased more than 8-fold compared to controls

abnormal trabecular bone morphology ( J:100918 )

• the bone marrow space is increased with only scattered thin spicules of trabecular bone trabecular number and thickness are decreased and trabecular separation is increased in the distal femur and proximal tibia

• however, there is no increase in bone marrow adiposity unlike Pthlh null homozygotes

abnormal bone ossification ( J:100918 )

• the mineral apposition rate is decreased by 45% and 60% in the tibial cortex and trabeculae, respectively

hematopoietic system

abnormal osteoclast morphology ( J:100918 )

• the number and surface of osteoclasts is reduced by 43% and 52%, respectively

decreased osteoclast cell number ( J:100918 )

immune system

abnormal osteoclast morphology ( J:100918 )

• the number and surface of osteoclasts is reduced by 43% and 52%, respectively

decreased osteoclast cell number ( J:100918 )

Genotype
MGI:5613584

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm4(Wnt7b)Flng}/Gt(ROSA)26Sor⁺; Tg(Col1a1-cre)1Ack/0
• Genetic Background: involves: FVB/N

skeleton

abnormal tibia morphology ( J:208766 )

• proximal tibial trabecular bone volume/total volume is increased 13.7 fold and 5.1 fold at 2 months and 6 months of age, respectively

• by 9 months of age bone mass of the proximal tibial trabecular area is 30% less than controls

abnormal bone structure ( J:208766 )

decreased osteoclast cell number ( J:208766 )

• osteoclast number per bone surface and percentage of bone resorption surface are reduced at 2 months of age

abnormal bone marrow cavity morphology ( J:208766 )

• absence of a marrow space in the long bones due to complete ossification

increased bone mineral density ( J:208766 )

• profoundly dense bones in both males and females throughout the body at 2 months of age

• increased density persists at 6 months of age but is partially resolved at 9 months of age

decreased trabecular bone volume ( J:208766 )

• by 9 months of age bone mass of the proximal tibial trabecular area is 30% less than controls

• however, bone mass remains increased in the distal tibia and femur at 9 months of age

increased trabecular bone volume ( J:208766 )

• proximal tibial trabecular bone volume/total volume is increased 13.7 fold and 5.1 fold at 2 months and 6 months of age, respectively

increased osteoblast cell number ( J:208766 )

• excessive osteoblasts in the presumptive marrow cavity at E18.5

• increased osteoblast number normalized to bone surface at 2 months of age

• however, the density of osteocytes is similar to controls

increased bone mass ( J:208766 )

• excessive bone occupies both primary and secondary ossification centers but the growth plate remains largely normal

• increased bone mass is seen at E18.5 and at 2 and 6 months of age

increased bone ossification ( J:208766 )

• mineral apposition rate, the percentage of mineralizing surface, and bone formation rate are all increased in the humerus

increased bone resorption ( J:208766 )

• increase in the serum levels of C-terminal telopeptide of type I collagen (a degradation product of type I collagen released upon bone resorption) at 1 and 9 months of age but not at 2 months of age

homeostasis/metabolism

increased circulating osteocalcin level ( J:208766 )

• increased at 1 and 2 months of age

increased circulating type I collagen C-terminal telopeptide level ( J:208766 )

• increase in the serum levels of C-terminal telopeptide of type I collagen (a degradation product of type I collagen released upon bone resorption) at 1 and 9 months of age but not at 2 months of age

hematopoietic system

enlarged spleen ( J:208766 )

• consistent with extramedullary hematopoiesis

increased spleen weight ( J:208766 )

decreased osteoclast cell number ( J:208766 )

• osteoclast number per bone surface and percentage of bone resorption surface are reduced at 2 months of age

immune system

enlarged spleen ( J:208766 )

• consistent with extramedullary hematopoiesis

increased spleen weight ( J:208766 )

decreased osteoclast cell number ( J:208766 )

• osteoclast number per bone surface and percentage of bone resorption surface are reduced at 2 months of age

limbs/digits/tail

abnormal tibia morphology ( J:208766 )

• proximal tibial trabecular bone volume/total volume is increased 13.7 fold and 5.1 fold at 2 months and 6 months of age, respectively

• by 9 months of age bone mass of the proximal tibial trabecular area is 30% less than controls

growth/size/body

enlarged spleen ( J:208766 )

• consistent with extramedullary hematopoiesis

increased spleen weight ( J:208766 )

Genotype
MGI:5613587

cn3

• Allelic Composition: Gt(ROSA)26Sor^{tm5(Wnt5a)Flng}/Gt(ROSA)26Sor^{tm5(Wnt5a)Flng}; Tg(Col1a1-cre)1Ack/0
• Genetic Background: involves: FVB/N

skeleton

skeleton phenotype ( J:208766 )

N • do not display any obvious bone phenotype at 2 months of age