Phenotypes associated with this allele

• Allele Symbol: Npm1^{Gt(VICTR37)704Lex}
• Allele Name: gene trap 704, Lexicon Genetics
• Allele ID: MGI:3604210
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Npm1^Gt(VICTR37)704Lex/Npm1^Gt(VICTR37)704Lex	involves: 129S5/SvEvBrd * C57BL/6	MGI:3608496
cx2	Npm1^Gt(VICTR37)704Lex/Npm1^Gt(VICTR37)704Lex Trp53^tm1Tyj/Trp53^tm1Tyj	involves: 129S2/SvPas * 129S5/SvEvBrd * C57BL/6	MGI:3608498

Genotype
MGI:3608496

hm1

• Allelic Composition: Npm1^{Gt(VICTR37)704Lex}/Npm1^{Gt(VICTR37)704Lex}
• Genetic Background: involves: 129S5/SvEvBrd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:101353 )

• all homozygotes die in utero between E9.5 and E10.5

cellular

increased apoptosis ( J:101353 )

• high proportions of caspase 3 positive cells and TRP53 positive cells are present in all tissues assessed

increased fibroblast apoptosis ( J:101353 )

• explanted fibroblasts undergo growth arrest in culture which can be reversed by disruption of TRP53

abnormal cell proliferation ( J:101353 )

• BrdU staining of embryos shows increased number of cycling cells relative to wild-type

embryo

embryo phenotype ( J:101353 )

N

embryonic growth arrest ( J:101353 )

• retarded growth and tissue differentiation and organization indicate developmental arrest

decreased embryo size ( J:101353 )

abnormal placenta development ( J:101353 )

• placental structures are normally developed, but smaller than normal

growth/size/body

decreased embryo size ( J:101353 )

Genotype
MGI:3608498

cx2

• Allelic Composition: Npm1^{Gt(VICTR37)704Lex}/Npm1^{Gt(VICTR37)704Lex}; Trp53^tm1Tyj/Trp53^tm1Tyj
• Genetic Background: involves: 129S2/SvPas * 129S5/SvEvBrd * C57BL/6

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:101353 )

• embryos are found up to E12.5 and these are larger than Npm1^{Gt(VICTR37)1Lex} homozygous embryos indicating a diminished severity in phenotype

cellular

abnormal apoptosis ( J:101353 )

• diminished caspase 3 positive cells in embryos relative to Npm1^{Gt(VICTR37)1Lex} homozygotes

increased fibroblast proliferation ( J:101353 )

• BrdU staining of embryos shows approximately 35% increase in fibroblasts of double homozygotes relative to Trp53^tm1Tyj homozygotes

• explanted fibroblasts show unlimited growth potential distinct from the arrested growth of Npm1^{Gt(VICTR37)1Lex} homozygotes