Phenotypes associated with this allele

• Allele Symbol: Scn9a^tm1Dgen
• Allele Name: targeted mutation 1, Deltagen
• Allele ID: MGI:3604573
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Scn9a^tm1Dgen/Scn9a^tm1Dgen	B6.129P2-Scn9a^tm1Dgen/J	MGI:5790336
hm2	Scn9a^tm1Dgen/Scn9a^tm1Dgen	C.129P2(B6)-Scn9a^tm1Dgen	MGI:5790340
hm3	Scn9a^tm1Dgen/Scn9a^tm1Dgen	either: (involves: 129P2/OlaHsd * BALB/c * C57BL/6) or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)	MGI:5790338
hm4	Scn9a^tm1Dgen/Scn9a^tm1Dgen	involves: 129P2/OlaHsd * C57BL/6	MGI:3606595
ht5	Scn9a^tm1Dgen/Scn9a^+	involves: 129P2/OlaHsd * C57BL/6	MGI:3606596

Genotype
MGI:5790336

hm1

• Allelic Composition: Scn9a^tm1Dgen/Scn9a^tm1Dgen
• Genetic Background: B6.129P2-Scn9a^tm1Dgen/J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:223425 )

• Background Sensitivity: no viable mice are obtained on a congenic C57BL/6 background, indicating neonatal lethality, compared to 20% survival on mixed backgrounds

Genotype
MGI:5790340

hm2

• Allelic Composition: Scn9a^tm1Dgen/Scn9a^tm1Dgen
• Genetic Background: C.129P2(B6)-Scn9a^tm1Dgen

mortality/aging

neonatal lethality, complete penetrance ( J:223425 )

• Background Sensitivity: nurturing of neonates on the congenic BALB/c background does not improve survival as it does on a mixed backgrounds

Genotype
MGI:5790338

hm3

• Allelic Composition: Scn9a^tm1Dgen/Scn9a^tm1Dgen
• Genetic Background: either: (involves: 129P2/OlaHsd * BALB/c * C57BL/6) or (involves: 129P2/OlaHsd * C57BL/6 * CD-1)

mortality/aging

postnatal lethality, incomplete penetrance ( J:223425 )

• Background Sensitivity: most mice on a mixed BALB/c background die before P14 while most mice on a mixed CD-1 background die before P20 compared to complete neonatal lethality of mice on a congenic C57BL/6 or BALB/c background

• Background Sensitivity: mice on either a mixed BALB/c or CD-1 background show an approximate 20% survival rate to adulthood when neonates are nurtured (by hand feeding, by receiving supplementary dextrose injections when dehydrated, and by culling litters to prevent competition for resources)

growth/size/body

decreased body size ( J:223425 )

• neonates are smaller than wild-type mice, however mice that survive to adulthood show normal body size

behavior/neurological

abnormal mechanical nociception ( J:223425 )

• in the tail-clip assay, mutants are insensitive to mechanical hyperalgesia and do not react to a clip placed on the tail

• however, mice show unchanged tactile sensitivity measured with von Frey fibers

abnormal nociception after inflammation ( J:223425 )

• mice lack a response to histamine, showing few to no scratching bouts in response to histamine injection, although a classic flare response at the injection site is seen

• 24 hours after injection of complete Freunds adjuvant into the paw, mutants do not develop thermal hyperalgesia at any time point measured in the Hargreaves apparatus as seen in controls

hypoalgesia ( J:223425 )

• mice do not respond to nociceptive stimuli

• however, mice exhibit normal overall locomotion and rearing

increased chemical nociceptive threshold ( J:223425 )

• mice exhibit reduced responses in both phases of the formalin pain assay, with licking and lifting in phase I of the formalin response being about 80% less in mutants and little to no licking and lifting was seen in phase II

• mice treated with veratradine or grayanotoxin III show reduced lifting and licking response, with an 85% and 96% lower response, respectively, than controls indicating that mice are insensitive to pain resulting from activation of peripheral sodium channels

increased thermal nociceptive threshold ( J:223425 )

• in the hot plate test, latency to withdrawal at each temperature is longer in mutants and mice do not display painful behaviors

• in the Hargreaves apparatus, mutant mice react with an approximate 40% increase in latency time compared to controls

• 24 hours after injection of complete Freunds adjuvant into the paw, mutants do not develop thermal hyperalgesia at any time point measured in the Hargreaves apparatus as seen in controls

immune system

dermatitis ( J:223425 )

• Background Sensitivity: in the early stages of production where F1 and F2 mice are bred, mice often show dermatitis

integument

dermatitis ( J:223425 )

• Background Sensitivity: in the early stages of production where F1 and F2 mice are bred, mice often show dermatitis

nervous system

abnormal action potential ( J:223425 )

• C-fiber spontaneous action potential frequency is 6-fold lower in saphenous nerve

• C-fiber action potential frequency is reduced in response to mechanical stimuli at the higher spiking rates evoked by stronger stimulus

abnormal synaptic transmission ( J:223425 )

• mice show reduced tetrodotoxin-sensitive sodium current recorded from sensory neuron cell bodies

renal/urinary system

abnormal renal/urinary system morphology ( J:223425 )

• Background Sensitivity: in the early stages of production where F1 and F2 mice are bred, some mice, mainly males, present with urinary issues such as prolapses and blockage

taste/olfaction

anosmia ( J:223425 )

• in a buried food assay, 18 of 19 mice do not find the hidden food pellet

Genotype
MGI:3606595

hm4

• Allelic Composition: Scn9a^tm1Dgen/Scn9a^tm1Dgen
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

mortality/aging

perinatal lethality, complete penetrance ( J:101679 )

• grossly normal homozygous embryos are identified at E12.5 to E18.5, but not at later stages

Genotype
MGI:3606596

ht5

• Allelic Composition: Scn9a^tm1Dgen/Scn9a⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

nervous system

increased prepulse inhibition ( J:101679 )

• exhibit significantly increased prepulse inhibition compared with age- and gender-matched wildtype controls