Phenotypes associated with this allele

• Allele Symbol: Prdm1^tm2Rob
• Allele Name: targeted mutation 2, Elizabeth J Robertson
• Allele ID: MGI:3605483
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Prdm1^tm2Rob/Prdm1^tm2Rob	involves: 129S/SvEv	MGI:5439605
hm2	Prdm1^tm2Rob/Prdm1^tm2Rob	involves: 129S/SvEv * C57BL/6	MGI:3606167
ht3	Prdm1^tm2Rob/Prdm1^+	involves: 129S/SvEv * C57BL/6	MGI:3606168
ht4	Prdm1^tm1.1Clme/Prdm1^tm2Rob	involves: 129S/Sv * 129X1/Sv * C57BL/6 * FVB/N * ICR	MGI:3606172
cx5	Kdr^tm1Jrt/Kdr^+ Prdm1^tm2Rob/Prdm1^tm2Rob	involves: 129	MGI:5439606

Genotype
MGI:5439605

hm1

• Allelic Composition: Prdm1^tm2Rob/Prdm1^tm2Rob
• Genetic Background: involves: 129S/SvEv

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

embryo

decreased trophoblast giant cell number ( J:187742 )

• SpA- trophoblast giant cells are entirely absent

abnormal spongiotrophoblast layer morphology ( J:187742 )

• abnormally flattened and unevenly distributed

abnormal placenta development ( J:187742 )

• Gcm1+ Syn-T cell population fails to involute and form characteristic long narrow branches

• the population of Gly-T cells become intermixed with cells of the compacted spongiotrophoblast layer

• the cone of invasive CK+ trophoblast cells is entirely absent and maternal AP+ cells become closely and inappropriately juxtaposed to the central maternal artery

cardiovascular system

uterine hemorrhage ( J:187742 )

♀ • extensive hemorrhaging of maternal blood within decidual tissues above the placenta at E9.5

reproductive system

uterine hemorrhage ( J:187742 )

♀ • extensive hemorrhaging of maternal blood within decidual tissues above the placenta at E9.5

Genotype
MGI:3606167

hm2

• Allelic Composition: Prdm1^tm2Rob/Prdm1^tm2Rob
• Genetic Background: involves: 129S/SvEv * C57BL/6

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:101718 )

• homozygous embryos die at E10.5

embryo

abnormal pharyngeal arch morphology ( J:101718 )

• at E9.5 a single branchial arch is present unlike wild-type mice which have 3 arches; however, specification and migration of neural crest cells is normal

• selective cell death occurs in the mesenchyme cells at the site where the 2nd and 3rd arches normally emerge

• however, in the limb bud patterning of the zone of polarizing activity and the apical ectodermal ridge is normal and the otic vesicle forms normally

abnormal pharyngeal arch mesenchyme morphology ( J:101718 )

• selective cell death occurs in the mesenchyme cells at the site where the 2nd and 3rd arches normally emerge

absent second pharyngeal arch ( J:101718 )

abnormal third pharyngeal arch morphology ( J:101718 )

decreased embryo size ( J:101718 )

• seen at E9.5

abnormal placenta labyrinth morphology ( J:101718 )

• at E9.5, the labyrinthine region fails to expand correctly reducing the volume of blood spaces

reproductive system

decreased primordial germ cell number ( J:101718 )

• at the headfold stage (E7.75) 2-4 or no primordial germs cells are detected and at E9.0-9.5, no primordial germ cells are detected

cardiovascular system

abnormal dorsal aorta morphology ( J:101718 )

• hemorrhaging with blood pooling in dorsal aorta is observed at E10.5

hemorrhage ( J:101718 )

• at E10.5, severe hemorrhaging is seen with blood pooling in the heart, dorsal aorta and under the surface ectoderm, but at E9.5 development of the heart and blood vessels appears normal

muscle

abnormal myotome development ( J:101718 )

• at E10.5, the Fgf8 domain in the epaxial dermomyotomal lip is expanded

growth/size/body

abnormal pharyngeal arch mesenchyme morphology ( J:101718 )

• selective cell death occurs in the mesenchyme cells at the site where the 2nd and 3rd arches normally emerge

decreased embryo size ( J:101718 )

• seen at E9.5

craniofacial

abnormal pharyngeal arch morphology ( J:101718 )

• at E9.5 a single branchial arch is present unlike wild-type mice which have 3 arches; however, specification and migration of neural crest cells is normal

• selective cell death occurs in the mesenchyme cells at the site where the 2nd and 3rd arches normally emerge

• however, in the limb bud patterning of the zone of polarizing activity and the apical ectodermal ridge is normal and the otic vesicle forms normally

abnormal pharyngeal arch mesenchyme morphology ( J:101718 )

• selective cell death occurs in the mesenchyme cells at the site where the 2nd and 3rd arches normally emerge

absent second pharyngeal arch ( J:101718 )

abnormal third pharyngeal arch morphology ( J:101718 )

cellular

decreased primordial germ cell number ( J:101718 )

• at the headfold stage (E7.75) 2-4 or no primordial germs cells are detected and at E9.0-9.5, no primordial germ cells are detected

Genotype
MGI:3606168

ht3

• Allelic Composition: Prdm1^tm2Rob/Prdm1⁺
• Genetic Background: involves: 129S/SvEv * C57BL/6

reproductive system

decreased primordial germ cell number ( J:101718 )

• at E7.75 and E9.0-9.5, the number of primordial germ cells is reduced and extrapolation indicates that the founding population of primordial germ cells is reduced by 78% compared to wild-type mice, 9 cells versus 40 cells in wild-type

cellular

decreased primordial germ cell number ( J:101718 )

• at E7.75 and E9.0-9.5, the number of primordial germ cells is reduced and extrapolation indicates that the founding population of primordial germ cells is reduced by 78% compared to wild-type mice, 9 cells versus 40 cells in wild-type

Genotype
MGI:3606172

ht4

• Allelic Composition: Prdm1^tm1.1Clme/Prdm1^tm2Rob
• Genetic Background: involves: 129S/Sv * 129X1/Sv * C57BL/6 * FVB/N * ICR

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:101718 )

• die at E10.5

embryo

abnormal pharyngeal arch morphology ( J:101718 )

• at E9.5 a single branchial arch is present unlike wild-type mice which have 3 arches; however, specification and migration of neural crest cells is normal

• selective cell death occurs in the mesenchyme cells at the site where the 2nd and 3rd arches normally emerge

• however, in the limb bud patterning of the zone of polarizing activity and the apical ectodermal ridge is normal and the otic vesicle forms normally

abnormal pharyngeal arch mesenchyme morphology ( J:101718 )

• selective cell death occurs in the mesenchyme cells at the site where the 2nd and 3rd arches normally emerge

absent second pharyngeal arch ( J:101718 )

• at E9.5

absent third pharyngeal arch ( J:101718 )

• at E9.5

decreased embryo size ( J:101718 )

• seen at E9.5

abnormal placenta labyrinth morphology ( J:101718 )

• at E9.5, the labyrinthine region fails to expand correctly reducing the volume of blood spaces

reproductive system

absent primordial germ cells ( J:101718 )

• at the headfold stage (E7.75) 2-4 or no primordial germs cells are detected and at E9.0-9.5, no primordial germ cells are detected

cardiovascular system

abnormal dorsal aorta morphology ( J:101718 )

• hemorrhaging with blood pooling in dorsal aorta is observed at E10.5

hemorrhage ( J:101718 )

• at E10.5, severe hemorrhaging is seen with blood pooling in the heart, dorsal aorta and under the surface ectoderm, but at E9.5 development of the heart and blood vessels appears normal

muscle

abnormal dermomyotome development ( J:101718 )

• at E10.5, the Fgf8 domain in the epaxial dermomyotomal lip is expanded

growth/size/body

abnormal pharyngeal arch mesenchyme morphology ( J:101718 )

• selective cell death occurs in the mesenchyme cells at the site where the 2nd and 3rd arches normally emerge

decreased embryo size ( J:101718 )

• seen at E9.5

craniofacial

abnormal pharyngeal arch morphology ( J:101718 )

• at E9.5 a single branchial arch is present unlike wild-type mice which have 3 arches; however, specification and migration of neural crest cells is normal

• selective cell death occurs in the mesenchyme cells at the site where the 2nd and 3rd arches normally emerge

• however, in the limb bud patterning of the zone of polarizing activity and the apical ectodermal ridge is normal and the otic vesicle forms normally

abnormal pharyngeal arch mesenchyme morphology ( J:101718 )

• selective cell death occurs in the mesenchyme cells at the site where the 2nd and 3rd arches normally emerge

absent second pharyngeal arch ( J:101718 )

• at E9.5

absent third pharyngeal arch ( J:101718 )

• at E9.5

cellular

absent primordial germ cells ( J:101718 )

• at the headfold stage (E7.75) 2-4 or no primordial germs cells are detected and at E9.0-9.5, no primordial germ cells are detected

Genotype
MGI:5439606

cx5

• Allelic Composition: Kdr^tm1Jrt/Kdr⁺; Prdm1^tm2Rob/Prdm1^tm2Rob
• Genetic Background: involves: 129

embryo

abnormal placental labyrinth vasculature morphology ( J:187742 )

• fetal capillaries fail to extend into the labyrinth tissue and instead are largely restricted to the base of the labyrinth

cardiovascular system

abnormal placental labyrinth vasculature morphology ( J:187742 )

• fetal capillaries fail to extend into the labyrinth tissue and instead are largely restricted to the base of the labyrinth