Phenotypes associated with this allele

• Allele Symbol: Tg(KRT5-cre)1Xya
• Allele Name: transgene insertion 1, Xiao Yang
• Allele ID: MGI:3606663
• Summary: 8 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Pten^tm2Mak/Pten^tm2Mak Tg(KRT5-cre)1Xya/0	involves: 129P2/OlaHsd	MGI:3607782
cn2	Pten^tm2Mak/Pten^tm2Mak Smad4^tm2.1Cxd/Smad4^tm2.1Cxd Tg(KRT5-cre)1Xya/0	involves: 129P2/OlaHsd * 129S6/SvEvTac	MGI:3607778
cn3	Smad4^tm2.1Cxd/Smad4^tm2.1Cxd Tg(KRT5-cre)1Xya/0	involves: 129S6/SvEvTac	MGI:3607725
cn4	Ppm1a^tm1Xya/Ppm1a^tm1Xya Tg(KRT5-cre)1Xya/0	involves: 129S6/SvEvTac	MGI:5304791
cn5	Smad2^tm1Xya/Smad2^tm1Xya Tg(KRT5-cre)1Xya/0	involves: 129S6/SvEvTac	MGI:5305135
cn6	Ppm1a^tm1.1Xya/Ppm1a^tm1.1Xya Smad2^tm1Xya/Smad2^tm1Xya Tg(KRT5-cre)1Xya/0	involves: 129S6/SvEvTac * FVB/N	MGI:5305136
cn7	Rad9a^tm2Lieb/Rad9a^tm2Lieb Tg(KRT5-cre)1Xya/?	involves: 129S/SvEv * C57BL/6	MGI:3809928
cn8	Rad9a^tm2Lieb/Rad9a^+ Tg(KRT5-cre)1Xya/?	involves: 129S/SvEv * C57BL/6	MGI:3809929

Genotype
MGI:3607782

cn1

• Allelic Composition: Pten^tm2Mak/Pten^tm2Mak; Tg(KRT5-cre)1Xya/0
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

integument

hair follicle outer root sheath hyperplasia ( J:101602 )

• hyperplastic outer root sheaths are seen at 3 months of age

epidermal hyperplasia ( J:101602 )

• observed at 3 months of age

Genotype
MGI:3607778

cn2

• Allelic Composition: Pten^tm2Mak/Pten^tm2Mak; Smad4^tm2.1Cxd/Smad4^tm2.1Cxd; Tg(KRT5-cre)1Xya/0
• Genetic Background: involves: 129P2/OlaHsd * 129S6/SvEvTac

digestive/alimentary system

increased stomach tumor incidence ( J:101602 )

• some die of stomach carcinomas by 3 months of age

mortality/aging

premature death ( J:101602 )

• some die by 3 months of age due to stomach carcinomas

neoplasm

increased stomach tumor incidence ( J:101602 )

• some die of stomach carcinomas by 3 months of age

increased skin tumor incidence ( J:101602 )

• tumor formation is accelerated in comparison to conditional keratinocyte targeted Smad4^tm2.1Cxd or Pten^tm2Mak mutant mice

increased skin papilloma incidence ( J:101602 )

• develop visible squamous papillomas on their backs at 2 months of age

increased carcinoma incidence ( J:101602 )

• some die of stomach carcinomas by 3 months of age

decreased tumor latency ( J:101602 )

• tumor formation is accelerated in comparison to conditional keratinocyte targeted Smad4^tm2.1Cxd or Pten^tm2Mak mutant mice

integument

increased keratinocyte proliferation ( J:101602 )

• exhibit persistently proliferating follicular keratinocytes

epithelioid cyst ( J:101602 )

• exhibit enlarged cysts surrounded by a multilayered epithelium filled with keratinized debris or pigments

alopecia ( J:101602 )

• exhibit obvious hair loss 2 months after birth, about 1 month earlier than conditional keratinocyte targeted Smad4^tm2.1Cxd mutant mice

thick hair follicle outer root sheath ( J:101602 )

• outer root sheath is thicker at P18

abnormal hair follicle regression ( J:101602 )

• hair follicles fail to go into catagen stage, indicating blocked regression

increased hair follicle cell proliferation ( J:101602 )

• hair follicles have significantly more proliferating cells at P18 than conditional keratinocyte targeted Pten^tm2Mak mutant mice

thick epidermis ( J:101602 )

• thickened epidermis is observed at P18

increased skin tumor incidence ( J:101602 )

• tumor formation is accelerated in comparison to conditional keratinocyte targeted Smad4^tm2.1Cxd or Pten^tm2Mak mutant mice

increased skin papilloma incidence ( J:101602 )

• develop visible squamous papillomas on their backs at 2 months of age

cellular

increased keratinocyte proliferation ( J:101602 )

• exhibit persistently proliferating follicular keratinocytes

growth/size/body

epithelioid cyst ( J:101602 )

• exhibit enlarged cysts surrounded by a multilayered epithelium filled with keratinized debris or pigments

Genotype
MGI:3607725

cn3

• Allelic Composition: Smad4^tm2.1Cxd/Smad4^tm2.1Cxd; Tg(KRT5-cre)1Xya/0
• Genetic Background: involves: 129S6/SvEvTac

mortality/aging

premature death ( J:101602 )

• do not survive more than 15 months

neoplasm

increased skin tumor incidence ( J:101602 )

• start to develop skin tumors around 5 months of age and by 12 months of age, 70% exhibit visible tumors

increased skin papilloma incidence ( J:101602 )

• most are well-differentiated squamous cell carcinomas and squamous papillomas

increased carcinoma incidence ( J:101602 )

• most are well-differentiated squamous cell carcinomas and squamous papillomas

integument

alopecia ( J:101602 )

• exhibit progressive hair loss that is first evident at P20, is obvious at 3 months of age, and generalized alopecia is seen by 7 months of age

abnormal hypodermis morphology ( J:101602 )

• cysts are seen in the hypodermis

abnormal hair follicle morphology ( J:101602 )

• increase in cell proliferation in hair follicle compared to controls at P25

• hair follicles are disordered and hypertrophied

thick hair follicle outer root sheath ( J:101602 )

• the outer root sheath is abnormally thick and exhibits an increase in cell proliferation in the first anagen at P15

enlarged hair follicles ( J:101602 )

• size of hair follicles is increased at 2 months of age

abnormal hair follicle regression ( J:101602 )

• hair follicles fail to regress and persist in an abnormal anagen phase; by P30 when a new anagen phase starts in controls, homozygotes exhibit thickened and distored follicles

• lower number of apoptotic cells in the hair bulb of early catagen hair follicles

thick epidermis stratum basale ( J:101602 )

• basal layer of the epidermis is thickened

hyperkeratosis ( J:101602 )

• severe hyperkeratosis in the esophagus at 7 months of age

abnormal keratinocyte morphology ( J:101602 )

• number of keratinocytes is increased in the epidermis and hair follicles

thick epidermis ( J:101602 )

• increase in epidermal proliferation

abnormal skin appearance ( J:101602 )

• black and white cysts are seen through out the skin surface

thick skin ( J:101602 )

• skin is thick and stiff

increased skin tumor incidence ( J:101602 )

• start to develop skin tumors around 5 months of age and by 12 months of age, 70% exhibit visible tumors

increased skin papilloma incidence ( J:101602 )

• most are well-differentiated squamous cell carcinomas and squamous papillomas

abnormal keratinocyte physiology ( J:101602 )

• response of keratinocytes to growth inhibition by TGF-beta1 is impaired

increased keratinocyte proliferation ( J:101602 )

• increase in keratinocyte proliferation

cellular

increased keratinocyte proliferation ( J:101602 )

• increase in keratinocyte proliferation

Genotype
MGI:5304791

cn4

• Allelic Composition: Ppm1a^tm1Xya/Ppm1a^tm1Xya; Tg(KRT5-cre)1Xya/0
• Genetic Background: involves: 129S6/SvEvTac

Delayed re-epithelialization during wound healing in Ppm1a^tm1Xya/Ppm1a^tm1Xya Tg(KRT5-cre)1Xya/0 mice

homeostasis/metabolism

abnormal wound healing ( J:178733 )

• mice exhibit delayed re-epithelialization and decreased keratinocyte migration during wound healing compared with control mice

delayed wound healing ( J:178733 )

integument

decreased keratinocyte migration ( J:178733 )

• during wound healing, keratinocyte migration is decreased compared to in control mice

cellular

decreased keratinocyte migration ( J:178733 )

• during wound healing, keratinocyte migration is decreased compared to in control mice

Genotype
MGI:5305135

cn5

• Allelic Composition: Smad2^tm1Xya/Smad2^tm1Xya; Tg(KRT5-cre)1Xya/0
• Genetic Background: involves: 129S6/SvEvTac

Accelerated re-epithelialization during wound healing in Smad2^tm1Xya/Smad2^tm1Xya Tg(KRT5-cre)1Xya/0 mice

homeostasis/metabolism

abnormal wound healing ( J:178733 )

• mice exhibit accelerated re-epithelialization and enhanced keratinocyte migration in wound healing compared with control mice

enhanced wound healing ( J:178733 )

integument

increased keratinocyte migration ( J:178733 )

• during wound healing, keratinocyte migration is increased compared to in control mice

cellular

increased keratinocyte migration ( J:178733 )

• during wound healing, keratinocyte migration is increased compared to in control mice

Genotype
MGI:5305136

cn6

• Allelic Composition: Ppm1a^tm1.1Xya/Ppm1a^tm1.1Xya; Smad2^tm1Xya/Smad2^tm1Xya; Tg(KRT5-cre)1Xya/0
• Genetic Background: involves: 129S6/SvEvTac * FVB/N

Re-epithelialization rate is accelerated in Smad2^tm1Xya/Smad2^tm1Xya Ppm1a^tm1.1Xya/Ppm1a^tm1.1Xya Tg(KRT5-cre)1Xya/0 mice

homeostasis/metabolism

abnormal wound healing ( J:178733 )

• mice exhibit accelerated re-epithelialization and enhanced keratinocyte migration in wound healing compared with control mice

enhanced wound healing ( J:178733 )

integument

increased keratinocyte migration ( J:178733 )

• during wound healing, keratinocyte migration is increased compared to control mice

cellular

increased keratinocyte migration ( J:178733 )

• during wound healing, keratinocyte migration is increased compared to control mice

Genotype
MGI:3809928

cn7

• Allelic Composition: Rad9a^tm2Lieb/Rad9a^tm2Lieb; Tg(KRT5-cre)1Xya/?
• Genetic Background: involves: 129S/SvEv * C57BL/6

neoplasm

increased skin papilloma incidence ( J:138845 )

• DMBA painted onto shaved skin 2X per week for 25 weeks

• induced by DMBA treatment in about 15 weeks as compared to 27 weeks for wild-type controls

• tumors derived from keratinocytes

• all tumor cells are conditionally deleted

cellular

abnormal cell cycle ( J:138845 )

• cell cycle arrest in late S phase

increased apoptosis ( J:138845 )

• in DMBA treated cells

abnormal double-strand DNA break repair ( J:138845 )

• more double strand breaks than wild-type controls

• DMBA increases double strand breaks

pigmentation

abnormal skin pigmentation ( J:138845 )

• due to DMBA treatment

• in 81% of homozygotes as compared to 13% of wild-type controls

• increased pigmentation within 2-5 weeks of tumor formation

homeostasis/metabolism

abnormal double-strand DNA break repair ( J:138845 )

• more double strand breaks than wild-type controls

• DMBA increases double strand breaks

integument

abnormal skin pigmentation ( J:138845 )

• due to DMBA treatment

• in 81% of homozygotes as compared to 13% of wild-type controls

• increased pigmentation within 2-5 weeks of tumor formation

increased skin papilloma incidence ( J:138845 )

• tumors derived from keratinocytes

• all tumor cells are conditionally deleted

• induced by DMBA treatment in about 15 weeks as compared to 27 weeks for wild-type controls

• DMBA painted onto shaved skin 2X per week for 25 weeks

Genotype
MGI:3809929

cn8

• Allelic Composition: Rad9a^tm2Lieb/Rad9a⁺; Tg(KRT5-cre)1Xya/?
• Genetic Background: involves: 129S/SvEv * C57BL/6

neoplasm

increased skin papilloma incidence ( J:138845 )

• induced by DMBA treatment in about 25 weeks for heterozygotes compared to 27 weeks for wild-type controls

• tumors derived from keratinocytes

• all tumor cells are conditionally deleted

• DMBA painted onto shaved skin 2X per week for 25 weeks

cellular

abnormal double-strand DNA break repair ( J:138845 )

• more double strand breaks than wild-type controls

pigmentation

abnormal skin pigmentation ( J:138845 )

• in 25% of heterozygotes compared to 13% of wild-type controls

• increased pigmentation within 2-5 weeks of tumor formation

• due to DMBA treatment

homeostasis/metabolism

abnormal double-strand DNA break repair ( J:138845 )

• more double strand breaks than wild-type controls

integument

abnormal skin pigmentation ( J:138845 )

• in 25% of heterozygotes compared to 13% of wild-type controls

• increased pigmentation within 2-5 weeks of tumor formation

• due to DMBA treatment

increased skin papilloma incidence ( J:138845 )

• induced by DMBA treatment in about 25 weeks for heterozygotes compared to 27 weeks for wild-type controls

• tumors derived from keratinocytes

• all tumor cells are conditionally deleted

• DMBA painted onto shaved skin 2X per week for 25 weeks