All Phenotypes Spag16<tm1Jfs> MGI Mouse

increased male germ cell apoptosis ( J:92446 )

• TUNEL staining revealed increased apoptosis, primarily at the round spermatid stage

abnormal sperm motility ( J:92446 )

• sperm motility of chimeras is decreased

abnormal seminiferous tubule morphology ( J:92446 )

abnormal Sertoli cell morphology ( J:92446 )

• Sertoli cells of some chimeras are vacuolated

seminiferous tubule degeneration ( J:92446 )

• chimeras contain some tubules with a central collection of degenerating cells, others have no germ cells, and a few show normal spermatogenesis

small testis ( J:92446 )

• testis of chimeras between 7 weeks and 8 months of age is smaller

abnormal spermatogenesis ( J:92446 )

abnormal outer dense fiber morphology ( J:92446 )

• some sperm show marked disorganization of the outer dense fibers

abnormal sperm axoneme morphology ( J:92446 )

• sperm from chimeras exhibit disorganized axonemes, some lacking the central pair of microtubules, others showing marked disorganization of the outer doublet microtubules and outer dense fibers

• however, there is a much smaller percentage of abnormal axonemes in the testis, suggesting a maturation-dependent change in axonemal structure

absent sperm axonemal central pair ( J:92446 )

• some sperm lack the central pair of microtubules

kinked sperm flagellum ( J:92446 )

• many epididymal sperm of chimeras have bent tails and display a quaking motion

oligozoospermia ( J:92446 )

• chimeras show a reduction in the number of sperm recovered from the cauda epididymis

arrest of spermatogenesis ( J:92446 )

• spermatogenesis in chimeras with degenerating germ cells is arrested at the round spermatid stage

male infertility ( J:92446 )

• most male chimeras are infertile while the others produce only non-mutant offspring