Phenotypes associated with this allele

• Allele Symbol: Tg(Tek-cre)1Rwng
• Allele Name: transgene insertion 1, Rong Wang
• Allele ID: MGI:3608912
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Itgb8^tm1Lfr/Itgb8^tm2Lfr Tg(Tek-cre)1Rwng/0	involves: 129/Sv * 129X1/SvJ * C57BL/6 * FVB/N * SJL	MGI:3609118
cn2	Myc^tm2.1Atp/Myc^tm2.1Atp Tg(Tek-cre)1Rwng/0	involves: 129P2/OlaHsd * FVB/N	MGI:3811916
cn3	Foxc1^tm1Tsku/Foxc1^tm1Blh Tg(Tek-cre)1Rwng/0	involves: 129S6/SvEvTac * FVB/N	MGI:3798681
cn4	Ptk2^tm1Lfr/Ptk2^tm1Lfr Tg(Tek-cre)1Rwng/0	involves: 129X1/SvJ * FVB/N	MGI:3689988

Genotype
MGI:3609118

cn1

• Allelic Composition: Itgb8^tm1Lfr/Itgb8^tm2Lfr; Tg(Tek-cre)1Rwng/0
• Genetic Background: involves: 129/Sv * 129X1/SvJ * C57BL/6 * FVB/N * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:102190 )

• no hemorrhages or other defects are seen in the brain and a normal vascular plexus develops in the brain

Genotype
MGI:3811916

cn2

• Allelic Composition: Myc^tm2.1Atp/Myc^tm2.1Atp; Tg(Tek-cre)1Rwng/0
• Genetic Background: involves: 129P2/OlaHsd * FVB/N

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:137630 )

• mice die at E11.5

liver/biliary system

abnormal liver morphology ( J:137630 )

• liver cells exhibit a mesenchymal rather than epithelial organization

small liver ( J:137630 )

liver hypoplasia ( J:137630 )

• the decrease in liver size is greater than can be numerically accounted for by hypoplasia

hematopoietic system

abnormal hematopoietic system morphology/development ( J:137630 )

• mice exhibit a hematopoietic phenotype identical to that observed in Myc^tm2.1Atp/Myc^tm2.1Atp Tg(Sox2-cre)1Amc mice

embryo

pale yolk sac ( J:137630 )

cardiovascular system

cardiovascular system phenotype ( J:137630 )

N • embryonic vasculature development is normal

Genotype
MGI:3798681

cn3

• Allelic Composition: Foxc1^tm1Tsku/Foxc1^tm1Blh; Tg(Tek-cre)1Rwng/0
• Genetic Background: involves: 129S6/SvEvTac * FVB/N

cellular

abnormal cell migration ( J:131323 )

• isolated pulmonary microvascular endothelial cells exhibit a significant reduction in chemotactic migration towards Cxcl12

Genotype
MGI:3689988

cn4

• Allelic Composition: Ptk2^tm1Lfr/Ptk2^tm1Lfr; Tg(Tek-cre)1Rwng/0
• Genetic Background: involves: 129X1/SvJ * FVB/N

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:104401 )

• lethality between E10.5 and E11.5

growth/size/body

decreased embryo size ( J:104401 )

• slightly smaller at E10.5

embryo

abnormal vitelline vasculature morphology ( J:104401 )

• yolk sacs exhibit wider capillary diameters, fewer small intercapillary spaces, and larger intercapillary areas that are often associated with incomplete vascular sprouts at E9.5

• yolk sacs lack the hierarchical vitelline vascular pattern and show only remnants of major vessels

disorganized yolk sac vascular plexus ( J:104401 )

• the capillary plexuses lack the intricate network structure and instead the microvessels are irregularly shaped, frequently dilated, and flattened with a sheet-like appearance and thin, spiky connections

decreased embryo size ( J:104401 )

• slightly smaller at E10.5

cardiovascular system

abnormal blood vessel morphology ( J:104401 )

• show patches of sequestered blood, reflecting dilated vessels, primarily in the upper trunk and head regions

• vessels in the head appear flat and fused to the surrounding widened and sinusoidal capillaries

• para-aortic splanchnopleural mesoderm explants undergo a process in which endothelial cells contract and cluster with each other, resulting in an irregular network composed of wider and smaller vessels

abnormal vascular endothelial cell morphology ( J:104401 )

• isolated endothelial cells grown on fibronectin are poorly spread, lack membrane ruffling and lamellipodia formation and show aberrant locomotion that is faster in the presence of serum and growth supplements

• endothelial cells show fewer and abnormal stress fibers that instead resemble cortical actin bundles and show fewer focal adhesions

abnormal capillary morphology ( J:104401 )

• vessels in the head appear flat and fused to the surrounding widened and sinusoidal capillaries, leading to a great variation in the size of the capillaries and intercapillary spaces

• allantoic explants and para-aortic splanchnopleural mesoderm explants show dilation of capillaries and intercapillary spaces with a reduction in network complexity

abnormal vascular regression ( J:104401 )

• endothelial cell explants show no decrease in proliferation or migration but show an increase in cell retraction and death leading to reduced vessel growth and increased vessel regression

decreased angiogenesis ( J:104401 )

• exhibit complete absence of blood vessels in the neuroepithelium at E10.5, indicating defective sprouting angiogenesis into the neuroepithelium

abnormal vitelline vasculature morphology ( J:104401 )

• yolk sacs exhibit wider capillary diameters, fewer small intercapillary spaces, and larger intercapillary areas that are often associated with incomplete vascular sprouts at E9.5

• yolk sacs lack the hierarchical vitelline vascular pattern and show only remnants of major vessels

disorganized yolk sac vascular plexus ( J:104401 )

• the capillary plexuses lack the intricate network structure and instead the microvessels are irregularly shaped, frequently dilated, and flattened with a sheet-like appearance and thin, spiky connections

hemorrhage ( J:104401 )

• hemorrhage is apparent in both the amniotic and yolk sac cavities

cellular

abnormal vascular regression ( J:104401 )

• endothelial cell explants show no decrease in proliferation or migration but show an increase in cell retraction and death leading to reduced vessel growth and increased vessel regression