Phenotypes associated with this allele

• Allele Symbol: Dsg2^tm1Leu
• Allele Name: targeted mutation 1, Rudolf E Leube
• Allele ID: MGI:3609221
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Dsg2^tm1Leu/Dsg2^tm1Leu	either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)	MGI:3611338
ht2	Dsg2^tm1Leu/Dsg2^+	either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)	MGI:3611339

Genotype
MGI:3611338

hm1

• Allelic Composition: Dsg2^tm1Leu/Dsg2^tm1Leu
• Genetic Background: either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality at implantation, complete penetrance ( J:102536 )

• all homozygous embryos die around implantation

cellular

abnormal cell death ( J:102536 )

• after 6 days in culture no viable homozygous ES cells are detected

decreased fibroblast proliferation ( J:102536 )

• proliferation of homozygous ES cells is reduced

Genotype
MGI:3611339

ht2

• Allelic Composition: Dsg2^tm1Leu/Dsg2⁺
• Genetic Background: either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6)

mortality/aging

embryonic lethality between implantation and somite formation, incomplete penetrance ( J:102536 )

• fewer than expected heterozygotes are found at E5.0, E5.5 and E6.5 with the expected ratio found only at E3.5

• Background Sensitivity: backcrossing to C57BL/6 for multiple generations resulted in a increased frequency of survival heterozygotes, with the proportion of heterozygotes increasing from 25.56% in F1 mice to 46.67% in F10 mice; however backcrossinf to 129/Sv did not increase survival