Phenotypes associated with this allele

• Allele Symbol: Pam^tm1Jep
• Allele Name: targeted mutation 1, John E Pintar
• Allele ID: MGI:3610996
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pam^tm1Jep/Pam^tm1Jep	involves: 129S7/SvEvBrd * C57BL/6J	MGI:3611270
ht2	Pam^tm1Jep/Pam^+	involves: 129S7/SvEvBrd	MGI:3611274
ht3	Pam^tm1Jep/Pam^+	involves: 129S7/SvEvBrd * C57BL/6J	MGI:3611273

Genotype
MGI:3611270

hm1

• Allelic Composition: Pam^tm1Jep/Pam^tm1Jep
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:103533 )

• death occurs between E14.5 and E15.5

embryo

umbilical vein stenosis ( J:103533 )

• at E13.5 the umbilical cord vein appears constricted

abnormal vitelline vasculature morphology ( J:103533 )

• at E13.5 yolk sac vessels are absent or poorly formed

homeostasis/metabolism

edema ( J:103533 )

• at E11.5 -E12.5 fluid accumulation is seen near the aorta and rib primordia and this accumulation becomes more severe with time

• at E14.5 edema is also seen in the pleural and peritoneal cavities

cardiovascular system

abnormal artery morphology ( J:103533 )

• thin arterial walls are seen in the thoracic, abdominal, and carotid arteries

umbilical vein stenosis ( J:103533 )

• at E13.5 the umbilical cord vein appears constricted

abnormal vitelline vasculature morphology ( J:103533 )

• at E13.5 yolk sac vessels are absent or poorly formed

respiratory system

abnormal bronchus epithelium morphology ( J:103533 )

• decreased thickness of the epithelial cells in the developing bronchi is seen at E14.5

Genotype
MGI:3611274

ht2

• Allelic Composition: Pam^tm1Jep/Pam⁺
• Genetic Background: involves: 129S7/SvEvBrd

homeostasis/metabolism

impaired glucose tolerance ( J:103533 )

• in 10 month old heterozygotes, glucose levels are elevated at 60 and 120 min after glucose challenge compared to wild-type mice at the same times, no such difference is seen in 2 month old heterozygotes

adipose tissue

increased total body fat amount ( J:103533 )

• body weight is similar to wild-type but 10 month old heterozygotes have increased adiposity

Genotype
MGI:3611273

ht3

• Allelic Composition: Pam^tm1Jep/Pam⁺
• Genetic Background: involves: 129S7/SvEvBrd * C57BL/6J

homeostasis/metabolism

impaired glucose tolerance ( J:103533 )

• in 10 month old heterozygotes, glucose levels are elevated at 60 and 120 min after glucose challenge compared to wild-type mice at the same times, no such difference is seen in 2 month old heterozygotes