Phenotypes associated with this allele

• Allele Symbol: Tg(Myh6-ERBB4)HT2Gass
• Allele Name: transgene insertion HT2, Martin Gassmann
• Allele ID: MGI:3611060
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Erbb4^tm1Grl/Erbb4^tm1Grl Tg(Myh6-ERBB4)HT2Gass/0	B6.Cg-Erbb4^tm1Grl Tg(Myh6-ERBB4)HT2Gass	MGI:5318192
cx2	Erbb4^tm1Grl/Erbb4^tm1Grl Tg(Myh6-ERBB4)HT2Gass/0	involves: 129S1/Sv * 129X1/SvJ	MGI:3690032

Genotype
MGI:5318192

cn1

• Allelic Composition: Erbb4^tm1Grl/Erbb4^tm1Grl; Tg(Myh6-ERBB4)HT2Gass/0
• Genetic Background: B6.Cg-Erbb4^tm1Grl Tg(Myh6-ERBB4)HT2Gass

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:182690 )

N • animals show no alterations in saccharin preference (hedonic behavior) test or resident intruder test (aggression)

impaired contextual conditioning behavior ( J:182690 )

impaired cued conditioning behavior ( J:182690 )

decreased anxiety-related response ( J:182690 )

• mice show lower anxiety than wild-type animals in the elevated-plus maze

increased locomotor activity ( J:182690 )

• animals exhibit significantly more locomotor activity than controls in open field test

nervous system

abnormal long-term potentiation ( J:182690 )

• enhanced LTP in hippocampal slices are resistant to reversal by treatment with recombinant Nrg-1beta protein

enhanced long-term potentiation ( J:182690 )

• significantly enhanced in hippocampal slices (EPSC amplitude 260% vs 180% wild-type)

decreased prepulse inhibition ( J:182690 )

• animals display deficit in PPI; reductions in PPI are more pronounced at lower prepulse sound levels than in controls

• responses are similar to PV-cre/Erbb4^fl mice

Genotype
MGI:3690032

cx2

• Allelic Composition: Erbb4^tm1Grl/Erbb4^tm1Grl; Tg(Myh6-ERBB4)HT2Gass/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

mortality/aging

perinatal lethality, incomplete penetrance ( J:84403 )

• fewer pups are born than expected

endocrine/exocrine glands

abnormal mammary gland development ( J:84403 )

♀ • mammary gland development often outpaces that of controls during puberty, however by 9-10 weeks of age, development of the ductal tree is similar to controls

abnormal mammary gland growth during pregnancy ( J:84403 )

♀ • during pregnancy, mammary lobuloalveoli fail to differentiate correctly

abnormal mammary gland alveolus morphology ( J:84403 )

♀ • inguinal mammary glands of mutant mothers 1 day postpartum show abnormal alveolar organization; alveolar epithelial cells do not form a coherent boundary and are not columnar, lipid globules fill most of the lumens and are also trapped inside epithelial cells

hypolactation ( J:84403 )

♀ • exhibit defective lactation and expression of major milk genes is 20-30% lower at day 1 of lactation

♀ • offspring of mutant mothers, irregardless of genotype, show perinatal lethality, with 82% of pups becoming emaciated and dying between birth and P10; pups survive when fostered with controls

nervous system

nervous system phenotype ( J:145464 )

N • myelin sheaths in the brain are of normal thickness

abnormal cerebellar granule layer morphology ( J:84403 )

• cerebellum exhibits increased numbers of large interneurons in the granule cell layer, however neuromuscular junctions develop normally

abnormal cranial nerve morphology ( J:84403 )

• at E17.5, ectopic nerve projections persist and result in an ectopic cranial nerve between the geniculate and trigeminal ganglia

reproductive system

abnormal mammary gland growth during pregnancy ( J:84403 )

♀ • during pregnancy, mammary lobuloalveoli fail to differentiate correctly

cardiovascular system

cardiovascular system phenotype ( J:84403 )

N • exhibit rescue of the cardiac defects observed in homozygous Erbb4 mutants

craniofacial

craniofacial phenotype ( J:84403 )

N • observe no obvious defects in cranial skeleton or inner ear, lower jaw or tooth development

embryo

abnormal cranial neural crest cell migration ( J:84403 )

• exhibit aberrant migration of a subpopulation of rhombomere 4 (r4) neural crest cells into r3-adjacent cranial mesenchyme

• by E11, show aberrantly projecting axons extending through r3-adjacent mesenchyme, connecting the geniculate/cochleovestibular ganglion complex with the trigeminal ganglion

integument

abnormal mammary gland development ( J:84403 )

♀ • mammary gland development often outpaces that of controls during puberty, however by 9-10 weeks of age, development of the ductal tree is similar to controls

abnormal mammary gland growth during pregnancy ( J:84403 )

♀ • during pregnancy, mammary lobuloalveoli fail to differentiate correctly

abnormal mammary gland alveolus morphology ( J:84403 )

♀ • inguinal mammary glands of mutant mothers 1 day postpartum show abnormal alveolar organization; alveolar epithelial cells do not form a coherent boundary and are not columnar, lipid globules fill most of the lumens and are also trapped inside epithelial cells

hypolactation ( J:84403 )

♀ • exhibit defective lactation and expression of major milk genes is 20-30% lower at day 1 of lactation

♀ • offspring of mutant mothers, irregardless of genotype, show perinatal lethality, with 82% of pups becoming emaciated and dying between birth and P10; pups survive when fostered with controls

respiratory system

lung inflammation ( J:132190 )

• mild signs of chronic lung inflammation, such as increased granulocyte number and reduced surfactant protein D expression, are observed

impaired lung alveolus development ( J:132190 )

• at 11-14 weeks of age, the volume density and thickness of alveolar septa are increased to ~120% of those in heterozygous control animals

• the number of alveoli is significantly reduced by 20% relative to that in heterozygous control animals, indicating alveolar simplification

abnormal pulmonary alveolar duct morphology ( J:132190 )

• at 11-14 weeks of age, the volume density of alveolar ductal space is reduced to 91% of that in heterozygous control animals

thick pulmonary interalveolar septum ( J:132190 )

• at 11-14 weeks of age, the thickness of alveolar septa is increased to ~120% of those in heterozygous control animals

abnormal respiratory function ( J:132190 )

• at 11-14 weeks of age, tidal midexpiratory flow is significantly reduced relative to that in heterozygous controls

decreased pulmonary respiratory rate ( J:132190 )

• at 11-14 weeks of age, respiratory frequency (breaths/min) is significantly reduced relative to that in heterozygous controls

increased airway responsiveness ( J:132190 )

• at 11-14 weeks of age, airway hyperresponsiveness is observed in response to methacholine (MCh) provocation at doses of 0.125, 0.5, and 1.0 ug

• however, no significant differences are detected at MCh doses of 0.25 ug and above 1 ug

abnormal surfactant secretion ( J:132190 )

• a minor downregulation of surfactant protein D expression is observed

immune system

increased granulocyte number ( J:132190 )

• at 11-14 weeks of age, the number of granulocytes in lung is significantly increased relative to that in heterozygous control animals

lung inflammation ( J:132190 )

• mild signs of chronic lung inflammation, such as increased granulocyte number and reduced surfactant protein D expression, are observed

hematopoietic system

increased granulocyte number ( J:132190 )

• at 11-14 weeks of age, the number of granulocytes in lung is significantly increased relative to that in heterozygous control animals

cellular

abnormal cranial neural crest cell migration ( J:84403 )

• exhibit aberrant migration of a subpopulation of rhombomere 4 (r4) neural crest cells into r3-adjacent cranial mesenchyme

• by E11, show aberrantly projecting axons extending through r3-adjacent mesenchyme, connecting the geniculate/cochleovestibular ganglion complex with the trigeminal ganglion