Phenotypes associated with this allele

• Allele Symbol: Gria2^tm3Rsp
• Allele Name: targeted mutation 3, Rolf Sprengel
• Allele ID: MGI:3611335
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gria2^tm3Rsp/Gria2^tm3Rsp Tg(Camk2a-cre)1Gsc/0	involves: 129S1/Sv * 129X1/SvJ * BALB/cJ * FVB/N	MGI:3701766
cn2	Gria2^tm3Rsp/Gria2^tm3Rsp Tg(Slc6a3-icre)9075Gsc/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N	MGI:3835858
cn3	Gria2^tm3Rsp/Gria2^tm3Rsp Tg(Gnrh1-cre)1Rsp/0	involves: C57BL/6	MGI:3612446
cn4	Gria2^tm3Rsp/Gria2^tm3Rsp Tg(Camk2a-cre)1Gsc/0	involves: C57BL/6 * FVB/N	MGI:3612398

Genotype
MGI:3701766

cn1

• Allelic Composition: Gria2^tm3Rsp/Gria2^tm3Rsp; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * BALB/cJ * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:111689 )

N

abnormal spatial reference memory ( J:111689 )

• reference memory is impaired; mice take longer to show improvement in performance in Y-maze test and additional training does not improve success rate compared to controls

abnormal spatial working memory ( J:111689 )

• working memory is impaired; T-maze performance is impaired compared to controls

nervous system

abnormal neuron differentiation ( J:111689 )

• almost no mitotic cells and few postmitotic cells are detected in subgranular zone (SGZ)

abnormal brain interneuron morphology ( J:111689 )

• a significant reduction in parvalbumin-positive interneurons in the dentate gyrus is observed in mutants

loss of hippocampal neurons ( J:111689 )

• loss of pyramidal hippocampal neurons in CA3 is detected with no loss apparent in Gria2^tm2Rsp/+ transgenic mice

abnormal excitatory postsynaptic currents ( J:111689 )

• EPSCs are significantly smaller in mutants than in controls

abnormal excitatory postsynaptic potential ( J:111689 )

• evoked field EPSPs are severely reduced, reaching only ~37% of control values

• stimulation strength needed to elicit presynaptic fiber volleys of given amplitudes are significantly increased vs controls

• synaptic excitability in increased compared to controls; threshold for generating population spike is reduced by 71%

• threshold for generating population spikes are reached in almost all experiments in controls while threshold is reached less than half of experiments for mutants

abnormal AMPA-mediated synaptic currents ( J:111689 )

• in conditional mice, quantification of inward rectifying currents in CA1 neurons at P42 shows a 20-fold increase vs control neurons

abnormal paired-pulse facilitation ( J:111689 )

• once a population spike is elicited in the first response, appearance of multiple spikes is always seen in second response

cellular

abnormal neuron differentiation ( J:111689 )

• almost no mitotic cells and few postmitotic cells are detected in subgranular zone (SGZ)

Genotype
MGI:3835858

cn2

• Allelic Composition: Gria2^tm3Rsp/Gria2^tm3Rsp; Tg(Slc6a3-icre)9075Gsc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * FVB/N

nervous system

abnormal glutamate-mediated receptor currents ( J:139693 )

• the ratio of AMPA/NMDA-mediated receptor currents in the ventral tegmental area is significantly reduced compared to controls

Genotype
MGI:3612446

cn3

• Allelic Composition: Gria2^tm3Rsp/Gria2^tm3Rsp; Tg(Gnrh1-cre)1Rsp/0
• Genetic Background: involves: C57BL/6

nervous system

abnormal AMPA-mediated synaptic currents ( J:103818 )

• glutamate-evoked currents in GnRH neurons are inwardly rectifying, with only a tiny outward current at a test potential of +20 mV, and smaller than in controls

behavior/neurological

decreased aggression towards male mice ( J:103818 )

• males do not attack a wild-type male intruder and females exhibit a decrease in aggressive behavior toward male intruders during 10-min test periods compared with controls

increased anxiety-related response ( J:103818 )

• in a dark-light box anxiety test, males exhibit a reduced number of exits and an increase in the latency of first exit, but spend a similar time in the lit compartment

abnormal pup retrieval ( J:103818 )

♀ • females fail to retrieve pups, however nursing is normal

abnormal sexual interaction ( J:103818 )

♂ • males rarely produce offspring due to decreased aggression and mounting behaviors

♂ • males spend a similar amount of time in the middle compartment of the open field with clean bedding but significantly less time in cage bedding containing female mouse urine, however are able to sense female phermones/odorants because time spent in compartment with female bedding increased compared to time spent with clean bedding

♂ • female bedding does not stimulate any sexual behavior in males

decreased mounting behavior ( J:103818 )

♂ • males show no interest in females and do not mount females during the night like controls

reduced male mating frequency ( J:103818 )

♂ • males show no interest in females and do not mount females during the night like controls

reproductive system

reproductive system phenotype ( J:103818 )

N • although males rarely produce offspring, this is due to decreased aggression and mounting behaviors and not due to reproductive system abnormalities as testes, spermatagonia, spermatids, spermatozoa, sperm motility and serum testosterone levels are no different from controls

N • females also exhibit normal ovarian morphology, litter sizes, fertility, and breeding behavior

Genotype
MGI:3612398

cn4

• Allelic Composition: Gria2^tm3Rsp/Gria2^tm3Rsp; Tg(Camk2a-cre)1Gsc/0
• Genetic Background: involves: C57BL/6 * FVB/N

behavior/neurological

abnormal learning/memory/conditioning ( J:103689 )

• exhibit decreased olfactory memory

abnormal olfactory discrimination memory ( J:103689 )

• exhibit more rapid odor learning and enhanced olfactory discrimination in a go/no-go operant conditioning task, and decreased olfactory memory

increased exploration in new environment ( J:103689 )

• exploratory behavior in an open field task is slightly increased

abnormal spatial learning ( J:103689 )

• acquisition of a nonolfactory hippocampus-dependent spatial learning task (elevated Y-maze) is slightly impaired

increased anxiety-related response ( J:103689 )

• dark/light box tests reveal increased anxiety

impaired coordination ( J:103689 )

• motor coordination in an accelerating rotarod is somewhat impaired