Phenotypes associated with this allele

• Allele Symbol: Pisd^{Gt1(Betageo)Jev}
• Allele Name: gene trap 1, Jean E Vance
• Allele ID: MGI:3612417
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pisd^Gt1(Betageo)Jev/Pisd^Gt1(Betageo)Jev	involves: 129S2/SvPas * C57BL/6	MGI:3616241
ht2	Pisd^Gt1(Betageo)Jev/Pisd^+	involves: 129S2/SvPas * C57BL/6	MGI:3616246

Genotype
MGI:3616241

hm1

• Allelic Composition: Pisd^{Gt1(Betageo)Jev}/Pisd^{Gt1(Betageo)Jev}
• Genetic Background: involves: 129S2/SvPas * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Pisd^{Gt1(Betageo)Jev}/Pisd^{Gt1(Betageo)Jev} embryos show absence of extraembryonic tissues and no differentiation of the three germ layers

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:104104 )

• embryos fail to develop beyond E8-10

cellular

abnormal mitochondrial morphology ( J:104104 )

• at E8, mitochondria are irregular in shape, less elongated and wider than wild-type mitochondria

• In E8-9 fibroblasts, mitochondria are dispersed throughout the cell, rather than concentrated around the nucleus

embryo

abnormal germ layer development ( J:104104 )

• at E8, differentiation into the three germ layers is not detectable

decreased embryo size ( J:104104 )

• at E9, mutant embryos are much smaller than wild-type or heterozygous embryos

embryo tissue necrosis ( J:104104 )

• at E9, tissue necrosis is apparent in the embryos

abnormal extraembryonic tissue morphology ( J:104104 )

• at E8 no extraembryonic tissue is visible

• maternal blood is in direct contact with the embryo

growth/size/body

decreased embryo size ( J:104104 )

• at E9, mutant embryos are much smaller than wild-type or heterozygous embryos

Genotype
MGI:3616246

ht2

• Allelic Composition: Pisd^{Gt1(Betageo)Jev}/Pisd⁺
• Genetic Background: involves: 129S2/SvPas * C57BL/6

homeostasis/metabolism

abnormal enzyme/coenzyme activity ( J:104104 )

• phosphatidylserine decarboxylase activity in liver, brain and testes is 40-50% lower than in wild-type adult mice: however, content of phosphaytidylcholine, phosphatidylserine and phosphatidylethanolamine is similar to wild-type

• cytidylyltransferase activity in the liver is increased by 100% compared to wild-type