Analysis Tools
mortality/aging
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• homozygotes are under-represented with about 35% appearing abnormal between E6.0 and E8.5 with 5% and 11% of severely affected homozygous embryos either resorbing or appearing as empty visceral yolk sacs, respectively, at E8.5
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embryo
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• at E6.5-E7.0 expression of endoderm markers suggests defects in anterior visceral endoderm movement
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• at E6.5-E7.0 expression of endoderm markers suggests loss of definitive endoderm is some embryos
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• at E6.75 and E7.5 expression of early mesoderm markers is reduced or absent in about 50-60% of embryos
• at E8.5, less severely affected embryos have abnormal mesoderm derivatives
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• at E8.5, less severely affected embryos have poorly differentiated anterior structures
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• at E8.0 in about 5% of embryos expression of T indicates partial axis duplication resulting in sections of duplicated notochord
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• at E7.0 about 60% of embryos show reduction or absence of definitive endoderm formation
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• at E7.0 about 60% of embryos show reduction or absence of definitive endoderm formation
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• at E8.0 in about 5% of embryos expression of T indicates partial axis duplication resulting in sections of duplicated notochord
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• at E6.75, 1 of 9 embryos had more intense posterior Nodal expression and sections of greatly expanded primitive streak formation
• at E6.75, 2of 9 embryos had a conical morphology, were filled with cells of mesodermal origin, and displayed circumferential upregulation of Nodal in the visceral endoderm suggesting that most of the epiblast had undergone streak formation and become mesoderm
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• at E8.5, less severely affected embryos have a prominent constriction at the embryonic/extraembryonic boundary
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• at E6.5-E7.0 expression of endoderm markers suggests defects in anterior visceral endoderm formation
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cellular
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• at E6.5-E7.0 expression of endoderm markers suggests defects in anterior visceral endoderm movement
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