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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Arpc3Tn(sb-lacZ,GFP)T5.117Jtak
transposon insertion T5.117, Junji Takeda
MGI:3613188
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Arpc3Tn(sb-lacZ,GFP)T5.117Jtak/Arpc3Tn(sb-lacZ,GFP)T5.117Jtak involves: C3H * C57BL * C57BL/6 * DBA MGI:3613197
hm2
Arpc3Tn(sb-lacZ,GFP)T5.117Jtak/Arpc3Tn(sb-lacZ,GFP)T5.117Jtak Not Specified MGI:3706576
ht3
Arpc3Tn(sb-lacZ,GFP)T5.117Jtak/Arpc3tm1Jtak involves: 129S4/SvJae * C57BL/6 MGI:3706577


Genotype
MGI:3613197
hm1
Allelic
Composition
Arpc3Tn(sb-lacZ,GFP)T5.117Jtak/Arpc3Tn(sb-lacZ,GFP)T5.117Jtak
Genetic
Background
involves: C3H * C57BL * C57BL/6 * DBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Arpc3Tn(sb-lacZ,GFP)T5.117Jtak mutation (1 available); any Arpc3 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• reduced body size is seen at 24 days of age but no other gross abnormalities are seen




Genotype
MGI:3706576
hm2
Allelic
Composition
Arpc3Tn(sb-lacZ,GFP)T5.117Jtak/Arpc3Tn(sb-lacZ,GFP)T5.117Jtak
Genetic
Background
Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Arpc3Tn(sb-lacZ,GFP)T5.117Jtak mutation (1 available); any Arpc3 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• no live homozygous embryos are detected at 5.5 dpc (days post coitum); blastocysts can be isolated at 3.5 dpc

embryo
• early stage blastocyts are indistinguishable from wild-type, but at later stages remain unexpanded at later stages of development
• when 3.5 dpc blastocysts are grown in culture, ~64% of blastocysts fail to hatch from their zona pellucida
• when mutant blastocysts are grown in culture after zona removal, blastocyst outgrowth is severely impaired even after 3 days in culture; cell number is comparable to wild-type blastocysts at 3 days in culture, indicating defect is due to abnormal spreading
• inner cell mass of homozygous blastocysts survive in culture, but growth is halted
• when grown on embryonic fibroblasts, ICM growth is delayed after 5 days compared to wild-type

cellular
• dynamics of actin accumulation are abnormal in mutant blastocysts at 3.5 dpc
• actin-rich adherent structures observed in abundance in normal cells during migration are drastically reduced at periphery of trophoblast cells in mutants
• cytoskeletal organization at cell periphery and actin net assembly are aberrant in mutant cells; actin filaments are <50 nm in diameter compared to 8 nm in wild-type cells




Genotype
MGI:3706577
ht3
Allelic
Composition
Arpc3Tn(sb-lacZ,GFP)T5.117Jtak/Arpc3tm1Jtak
Genetic
Background
involves: 129S4/SvJae * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Arpc3tm1Jtak mutation (1 available); any Arpc3 mutation (12 available)
Arpc3Tn(sb-lacZ,GFP)T5.117Jtak mutation (1 available); any Arpc3 mutation (12 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• no live embryos are detected at 5.5 dpc; blastocysts can be isolated at 3.5 dpc

embryo
• in culture, 22% of blastocysts fail to hatch, while remainder show impaired outgrowth activity





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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory