Phenotypes associated with this allele

• Allele Symbol: Tg(CD2-Tgfbr2)1Grs
• Allele Name: transgene insertion 1, Ronald E Gress
• Allele ID: MGI:3614440
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(CD2-Tgfbr2)1Grs/0	C57BL/6-Tg(CD2-Tgfbr2)1Grs/Nci	MGI:3614558
tg2	Tg(CD2-Tgfbr2)1Grs/?	C57BL/6-Tg(CD2-Tgfbr2)1Grs/Nci	MGI:3614559

Genotype
MGI:3614558

tg1

• Allelic Composition: Tg(CD2-Tgfbr2)1Grs/0
• Genetic Background: C57BL/6-Tg(CD2-Tgfbr2)1Grs/Nci

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased T cell proliferation ( J:97523 )

• by 12-24 weeks of age, CD8⁺ numbers begin to rise in transgenic mice such that eventually cells from the spleen and lymph nodes (LN) of transgenic mice contain up to 10-fold the numbers of CD8⁺ T cells seen in splenocytes and LN cells of control mice, whereas absolute numbers of splenic and LN CD4⁺ T cells and of B cells in transgenic mice are similar to or lower than in control mice

• the CD4⁺/CD8⁺ T cell ratio of transgenic mice between 12 and 24 weeks of age becomes severely skewed in favor of CD8⁺ T cells due to the expansion of CD8⁺ cells

• a significantly higher proportion of lymph node T cells is cycling in transgenic than in littermate control mice

• the proportion of peripheral CD8⁺ T cells that are acively cycling in transgenic mice reaches the level (5-8%) seen in thymi of both transgenic and wild-type mice

• over time, the expanded CD8⁺ T cell population in transgenic mice progresses from polyclonal to oligoclonal - representing several clonal lineages - to, by 6-12 months of age, monoclonal or biclonal, based on the T cell beta variable region (TCR-Vb) repertoire of the population

• moderate infiltration of the heart, liver and lungs with small lymphocytes occurs in transgenic mice; however, inflammation is minimal

enlarged spleen ( J:97523 )

• spleens of transgenic mice become enlarged, with marked infiltration by small lymphocytes

enlarged lymph nodes ( J:97523 )

• lymph nodes of transgenic mice become enlarged, with marked infiltration by small lymphocytes

hematopoietic system

increased T cell proliferation ( J:97523 )

• by 12-24 weeks of age, CD8⁺ numbers begin to rise in transgenic mice such that eventually cells from the spleen and lymph nodes (LN) of transgenic mice contain up to 10-fold the numbers of CD8⁺ T cells seen in splenocytes and LN cells of control mice, whereas absolute numbers of splenic and LN CD4⁺ T cells and of B cells in transgenic mice are similar to or lower than in control mice

• the CD4⁺/CD8⁺ T cell ratio of transgenic mice between 12 and 24 weeks of age becomes severely skewed in favor of CD8⁺ T cells due to the expansion of CD8⁺ cells

• a significantly higher proportion of lymph node T cells is cycling in transgenic than in littermate control mice

• the proportion of peripheral CD8⁺ T cells that are acively cycling in transgenic mice reaches the level (5-8%) seen in thymi of both transgenic and wild-type mice

• over time, the expanded CD8⁺ T cell population in transgenic mice progresses from polyclonal to oligoclonal - representing several clonal lineages - to, by 6-12 months of age, monoclonal or biclonal, based on the T cell beta variable region (TCR-Vb) repertoire of the population

• moderate infiltration of the heart, liver and lungs with small lymphocytes occurs in transgenic mice; however, inflammation is minimal

enlarged spleen ( J:97523 )

• spleens of transgenic mice become enlarged, with marked infiltration by small lymphocytes

cellular

increased T cell proliferation ( J:97523 )

• by 12-24 weeks of age, CD8⁺ numbers begin to rise in transgenic mice such that eventually cells from the spleen and lymph nodes (LN) of transgenic mice contain up to 10-fold the numbers of CD8⁺ T cells seen in splenocytes and LN cells of control mice, whereas absolute numbers of splenic and LN CD4⁺ T cells and of B cells in transgenic mice are similar to or lower than in control mice

• the CD4⁺/CD8⁺ T cell ratio of transgenic mice between 12 and 24 weeks of age becomes severely skewed in favor of CD8⁺ T cells due to the expansion of CD8⁺ cells

• a significantly higher proportion of lymph node T cells is cycling in transgenic than in littermate control mice

• the proportion of peripheral CD8⁺ T cells that are acively cycling in transgenic mice reaches the level (5-8%) seen in thymi of both transgenic and wild-type mice

• over time, the expanded CD8⁺ T cell population in transgenic mice progresses from polyclonal to oligoclonal - representing several clonal lineages - to, by 6-12 months of age, monoclonal or biclonal, based on the T cell beta variable region (TCR-Vb) repertoire of the population

• moderate infiltration of the heart, liver and lungs with small lymphocytes occurs in transgenic mice; however, inflammation is minimal

growth/size/body

enlarged spleen ( J:97523 )

• spleens of transgenic mice become enlarged, with marked infiltration by small lymphocytes

Genotype
MGI:3614559

tg2

• Allelic Composition: Tg(CD2-Tgfbr2)1Grs/?
• Genetic Background: C57BL/6-Tg(CD2-Tgfbr2)1Grs/Nci

hematopoietic system

increased T cell proliferation ( J:93665 )

• leukocyte counts in peripheral blood from transgenic mice increase progressively over time, and they rise dramatically after 30 weeks; flow cytometric analysis reveals most of the increase to be in the CD8⁺ T cell population

• when transgenic mice have become symptomatic, histologic examination reveals infiltration and perivascular accumulation of lymphocytes in nonlymphoid organs

increased T cell derived lymphoma incidence ( J:93665 )

• by the time transgenic mice become symptomatic, their spleens and lymph nodes are greatly (often 10-fold) enlarged and the organs' structure and integrity have been disrupted due to heavy lymphocyte infiltration

abnormal CD8-positive, alpha beta T cell morphology ( J:93665 )

• by between 6 and 12 weeks of age, the memory T cell suface markers CD44 and interleukin-2 receptor beta (IL-2Rb) become greatly elevated on transgenic CD8⁺ T cells, and by 12 weeks the levels are greater than in 60 week old wild-type mice; levels of CD25, CD62L, CD69 and CD45Rb are generally unaffected, as are surface markers of CD4⁺ T cells

immune system

increased T cell proliferation ( J:93665 )

• leukocyte counts in peripheral blood from transgenic mice increase progressively over time, and they rise dramatically after 30 weeks; flow cytometric analysis reveals most of the increase to be in the CD8⁺ T cell population

• when transgenic mice have become symptomatic, histologic examination reveals infiltration and perivascular accumulation of lymphocytes in nonlymphoid organs

increased T cell derived lymphoma incidence ( J:93665 )

• by the time transgenic mice become symptomatic, their spleens and lymph nodes are greatly (often 10-fold) enlarged and the organs' structure and integrity have been disrupted due to heavy lymphocyte infiltration

abnormal CD8-positive, alpha beta T cell morphology ( J:93665 )

• by between 6 and 12 weeks of age, the memory T cell suface markers CD44 and interleukin-2 receptor beta (IL-2Rb) become greatly elevated on transgenic CD8⁺ T cells, and by 12 weeks the levels are greater than in 60 week old wild-type mice; levels of CD25, CD62L, CD69 and CD45Rb are generally unaffected, as are surface markers of CD4⁺ T cells

behavior/neurological

lethargy ( J:93665 )

• late in disease progression, transgenic mice become lethargic; homozygous mice become symptomatic approximately 4 months earlier than mice hemizygous for the transgene

growth/size/body

cachexia ( J:93665 )

• late in disease progression, transgenic mice exhibit weight loss; homozygous mice become symptomatic approximately 4 months earlier than mice hemizygous for the transgene

respiratory system

respiratory distress ( J:93665 )

• late in disease progression, transgenic mice exhibit breathing difficulty; homozygous mice become symptomatic approximately 4 months earlier than mice hemizygous for the transgene

neoplasm

increased T cell derived lymphoma incidence ( J:93665 )

• by the time transgenic mice become symptomatic, their spleens and lymph nodes are greatly (often 10-fold) enlarged and the organs' structure and integrity have been disrupted due to heavy lymphocyte infiltration

increased leukemia incidence ( J:93665 )

• progression to leukemia is associated with clonal expansion of CD8⁺ T cells, evidenced by a single predominant T cell receptor beta (TCR-Vb) rearrangement in the expanded CD8⁺ T cell population of every transgenic mouse with histologically confirmed leukemia; specific rearrangements differ among individual mice

cellular

increased T cell proliferation ( J:93665 )

• leukocyte counts in peripheral blood from transgenic mice increase progressively over time, and they rise dramatically after 30 weeks; flow cytometric analysis reveals most of the increase to be in the CD8⁺ T cell population

• when transgenic mice have become symptomatic, histologic examination reveals infiltration and perivascular accumulation of lymphocytes in nonlymphoid organs

endocrine/exocrine glands

increased T cell derived lymphoma incidence ( J:93665 )

• by the time transgenic mice become symptomatic, their spleens and lymph nodes are greatly (often 10-fold) enlarged and the organs' structure and integrity have been disrupted due to heavy lymphocyte infiltration