Phenotypes associated with this allele

• Allele Symbol: Gpr3^tm1Cled
• Allele Name: targeted mutation 1, Catherine Ledent
• Allele ID: MGI:3615760
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gpr3^tm1Cled/Gpr3^tm1Cled	either: B6.129-Gpr3^tm1Cled or (involves: 129S1/Sv * 129X1/SvJ * CD-1)	MGI:3618230

Genotype
MGI:3618230

hm1

• Allelic Composition: Gpr3^tm1Cled/Gpr3^tm1Cled
• Genetic Background: either: B6.129-Gpr3^tm1Cled or (involves: 129S1/Sv * 129X1/SvJ * CD-1)

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Oocytes from superovulated Gpr3^tm1Cled/Gpr3^tm1Cled females display age-dependent increase in fragmentation

reproductive system

abnormal oogenesis ( J:105182 )

♀

abnormal oocyte morphology ( J:105182 )

♀ • superovulated homozygous females display an age-dependent increase of fragmented oocytes, however the number of oocytes produced per ovulating mouse is similar to wild-type

abnormal female meiosis ( J:105182 )

♀ • oocytes at the GV stage display impaired meiotic competence and during culture ex vivo, only 50% of oocytes resume meiosis spontaneously compared with 80% of wild-type

♀ • about 1/3 of antral stage oocytes exhibit premature progression through meiosis resulting in precocious ripening

abnormal female reproductive system physiology ( J:105182 )

♀

short estrous cycle ( J:105182 )

♀ • shorter estrous cycles (4.3 days vs. 5.3 in wild-type)

reduced female fertility ( J:105182 )

♀ • age-related reproductive failure due to ovulation of a progressively increasing proportion of developmentally incompetent oocytes

decreased litter size ( J:105182 )

• progressive reduction in litter size with advancing maternal age, with the reduction independent of the sperm genotype

homeostasis/metabolism

increased circulating follicle stimulating hormone level ( J:105182 )

• in females at 8.5 months of age during diestrus

cellular

abnormal oogenesis ( J:105182 )

♀

abnormal oocyte morphology ( J:105182 )

♀ • superovulated homozygous females display an age-dependent increase of fragmented oocytes, however the number of oocytes produced per ovulating mouse is similar to wild-type

abnormal female meiosis ( J:105182 )

♀ • oocytes at the GV stage display impaired meiotic competence and during culture ex vivo, only 50% of oocytes resume meiosis spontaneously compared with 80% of wild-type

♀ • about 1/3 of antral stage oocytes exhibit premature progression through meiosis resulting in precocious ripening

maternal effect ( J:105182 )

• embryos from homozygous mothers complete the transition from zygote to blastocyst with significantly lower frequency than controls, however those that succeed in reaching the blastocyst stage, implant and develop normally

• progressive appearance with age of degenerated and/or fragmented embryos, all along preimplantation development