Phenotypes associated with this allele

• Allele Symbol: Rb1^tm1Dwg
• Allele Name: targeted mutation 1, David W Goodrich
• Allele ID: MGI:3617652
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rb1^tm1Dwg/Rb1^tm1Dwg	involves: 129/Sv * C57BL/6	MGI:3618355
ht2	Rb1^tm1Dwg/Rb1^tm1Tyj	involves: 129/Sv * 129S2/SvPas * C57BL/6	MGI:3618362
cn3	Rb1^tm1Dwg/Rb1^tm2Brn Tg(Chx10-EGFP/cre,-ALPP)2Clc/0	involves: 129/Sv * C57BL/6 * SJL	MGI:3618365

Genotype
MGI:3618355

hm1

• Allelic Composition: Rb1^tm1Dwg/Rb1^tm1Dwg
• Genetic Background: involves: 129/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:105548 )

• a few live homozygotes can be recovered as late as E17.5 (2 of 18 viable), indicating that mutants survive at least 2 days longer than homozygous Rb1^tm1Tyj mice

cellular

abnormal cell cycle ( J:105548 )

• MEFs exhibit an increase in the fraction of cells in the S and G2/M phases of the cell cycle

abnormal cell cycle checkpoint function ( J:105548 )

• MEFs fail to efficiently trigger G1/S cell cycle arrest in response to DNA damage

increased lens fiber apoptosis ( J:105548 )

• apoptosis is detected in the lens fiber compartment that is not seen in wild-type

increased fibroblast proliferation ( J:105548 )

• MEFs cultured at confluence exhibit an increase in cell proliferation compared to wild-type MEFs

embryo

decreased embryo size ( J:105548 )

• although embryos are smaller than wild-type, they are larger than age-matched homozygous Rb1^tm1Tyj embryos

abnormal placenta labyrinth morphology ( J:105548 )

• normal labyrinth architecture is disrupted

• the porous appearance of the labyrinth layer is absent

abnormal placental transport ( J:105548 )

• exhibit defective placental transport as indicated by a 7.7% reduction of the essential fatty acid linoleic acid, arachidonic acid and docosahexaenoic acid in E14.5 embryos relative to wild-type

hematopoietic system

abnormal erythropoiesis ( J:105548 )

• small percentage of erythrocytes exhibit enucleating defects at E17.5, indicating a less severe erythrocyte maturation defect than seen in homozygous Rb1^tm1Tyj mice

abnormal macrophage morphology ( J:105548 )

• fetal liver macrophages show fewer defects in differentiation compared to homozygous Rb1^tm1Tyj mice

vision/eye

increased lens fiber apoptosis ( J:105548 )

• apoptosis is detected in the lens fiber compartment that is not seen in wild-type

abnormal lens fiber morphology ( J:105548 )

• detect ectopic mitoses in the lens fiber compartment that is not seen in wild-type

• lens fiber cells are disorganized

nervous system

abnormal fourth ventricle morphology ( J:105548 )

• detect ectopic mitosis and apoptosis in the intermediate zones of the fourth ventricle

abnormal third ventricle morphology ( J:105548 )

• detect ectopic mitosis and apoptosis in the intermediate zones of the third ventricle

abnormal trigeminal ganglion morphology ( J:105548 )

• detect ectopic mitosis and apoptosis in the trigeminal ganglia

abnormal dorsal root ganglion morphology ( J:105548 )

• detect ectopic mitosis and apoptosis in the dorsal root ganglia

immune system

abnormal macrophage morphology ( J:105548 )

• fetal liver macrophages show fewer defects in differentiation compared to homozygous Rb1^tm1Tyj mice

growth/size/body

decreased embryo size ( J:105548 )

• although embryos are smaller than wild-type, they are larger than age-matched homozygous Rb1^tm1Tyj embryos

integument

pallor ( J:105548 )

• although embryos are paler than wild-type, they exhibit improved coloration over that seen in homozygous Rb1^tm1Tyj embryos

Genotype
MGI:3618362

ht2

• Allelic Composition: Rb1^tm1Dwg/Rb1^tm1Tyj
• Genetic Background: involves: 129/Sv * 129S2/SvPas * C57BL/6

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:105548 )

• survive as long as homozygous Rb1^tm1Dwg mice, with 2 of 18 dead at E15.5 and 4 of 7 dead at E17.5

growth/size/body

decreased embryo size ( J:105548 )

• although embryos are smaller than wild-type, they are larger than age-matched homozygous Rb1^tm1Tyj embryos

hematopoietic system

abnormal erythropoiesis ( J:105548 )

• small percentage of erythrocytes exhibit enucleating defects at E17.5, indicating a less severe erythrocyte maturation defect than seen in homozygous Rb1^tm1Tyj mice

embryo

decreased embryo size ( J:105548 )

• although embryos are smaller than wild-type, they are larger than age-matched homozygous Rb1^tm1Tyj embryos

integument

pallor ( J:105548 )

• although embryos are paler than wild-type, they exhibit improved coloration over that seen in homozygous Rb1^tm1Tyj embryos

Genotype
MGI:3618365

cn3

• Allelic Composition: Rb1^tm1Dwg/Rb1^tm2Brn; Tg(Chx10-EGFP/cre,-ALPP)2Clc/0
• Genetic Background: involves: 129/Sv * C57BL/6 * SJL

vision/eye

decreased retina rod cell number ( J:105548 )

• significant reduction in rod photoreceptors at P14, however no changes in the number of other major cell types in the retina

abnormal retina horizontal cell morphology ( J:105548 )

• horizontal cell synaptogenesis in the retina is defective

nervous system

decreased retina rod cell number ( J:105548 )

• significant reduction in rod photoreceptors at P14, however no changes in the number of other major cell types in the retina

abnormal retina horizontal cell morphology ( J:105548 )

• horizontal cell synaptogenesis in the retina is defective