Phenotypes associated with this allele

• Allele Symbol: Tgif1^tm1Dwot
• Allele Name: targeted mutation 1, David Wotton
• Allele ID: MGI:3617788
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tgif1^tm1Dwot/Tgif1^tm1Dwot	involves: 129X1/SvJ * C57BL/6J	MGI:3618103
ht2	Tgif1^tm1Dwot/Tgif1^+	involves: 129X1/SvJ * C57BL/6J	MGI:3618104
cx3	Nodal^tm1Rob/Nodal^+ Tgif1^tm1Dwot/Tgif1^tm1Dwot Tgif2^tm1Dwot/Tgif2^tm1Dwot	involves: 129S/SvEv * 129X1/SvJ * C57BL/6	MGI:4437425
cx4	Tgif1^tm1Dwot/Tgif1^tm1Dwot Tgif2^tm1Dwot/Tgif2^+	involves: 129S/SvEv * 129X1/SvJ * C57BL/6J	MGI:4437422
cx5	Tgif1^tm1Dwot/Tgif1^+ Tgif2^tm1Dwot/Tgif2^tm1Dwot	involves: 129S/SvEv * 129X1/SvJ * C57BL/6J	MGI:4437421
cx6	Tgif1^tm1Dwot/Tgif1^tm1Dwot Tgif2^tm1Dwot/Tgif2^tm1Dwot	involves: 129S/SvEv * 129X1/SvJ * C57BL/6J	MGI:4437423

Genotype
MGI:3618103

hm1

• Allelic Composition: Tgif1^tm1Dwot/Tgif1^tm1Dwot
• Genetic Background: involves: 129X1/SvJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

abnormal response to vitamins ( J:105576 )

• rate of retinoic-acid induced teratogenesis increases to 67% compared to wild-type when retinoic acid treatment is performed on E7.5

nervous system

exencephaly ( J:105576 )

• rate of retinoic-acid induced exencephaly is 56% while in wild-type it is 6% when treatment is performed on E7.5

Genotype
MGI:3618104

ht2

• Allelic Composition: Tgif1^tm1Dwot/Tgif1⁺
• Genetic Background: involves: 129X1/SvJ * C57BL/6J

homeostasis/metabolism

abnormal response to vitamins ( J:105576 )

• rate of retinoic-acid induced teratogenesis increases to 60% compared to wild-type when retinoic acid treatment is performed on E7.5

nervous system

exencephaly ( J:105576 )

• rate of retinoic-acid induced exencephaly is 56% while it is 6% in wild-type with treatment performed on E7.5

Genotype
MGI:4437425

cx3

• Allelic Composition: Nodal^tm1Rob/Nodal⁺; Tgif1^tm1Dwot/Tgif1^tm1Dwot; Tgif2^tm1Dwot/Tgif2^tm1Dwot
• Genetic Background: involves: 129S/SvEv * 129X1/SvJ * C57BL/6

embryo

embryo phenotype ( J:157256 )

N • unlike in double null mice wild-type for Nodal, the embryonic cavity has formed by E8.5 and a distinct AP axis is present

Genotype
MGI:4437422

cx4

• Allelic Composition: Tgif1^tm1Dwot/Tgif1^tm1Dwot; Tgif2^tm1Dwot/Tgif2⁺
• Genetic Background: involves: 129S/SvEv * 129X1/SvJ * C57BL/6J

embryo

abnormal proximal-distal axis patterning ( J:157256 )

• a small proportion of embryos at E6.5 have a rounder appearance suggesting decreased elongation of the proximodistal axis

growth/size/body

decreased body size ( J:157256 )

• tend to be smaller than their littermates

reproductive system

reduced fertility ( J:157256 )

• often show reduced fertility

Genotype
MGI:4437421

cx5

• Allelic Composition: Tgif1^tm1Dwot/Tgif1⁺; Tgif2^tm1Dwot/Tgif2^tm1Dwot
• Genetic Background: involves: 129S/SvEv * 129X1/SvJ * C57BL/6J

reproductive system

reduced fertility ( J:157256 )

• often show reduced fertility

growth/size/body

decreased body size ( J:157256 )

• tend to be smaller than their littermates

embryo

abnormal proximal-distal axis patterning ( J:157256 )

• a small proportion of embryos at E6.5 have a rounder appearance suggesting decreased elongation of the proximodistal axis

Genotype
MGI:4437423

cx6

• Allelic Composition: Tgif1^tm1Dwot/Tgif1^tm1Dwot; Tgif2^tm1Dwot/Tgif2^tm1Dwot
• Genetic Background: involves: 129S/SvEv * 129X1/SvJ * C57BL/6J

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:157256 )

• unable to identify any embryos after E9.5

growth/size/body

decreased body size ( J:157256 )

• significantly smaller at E7.5 and E8.5

embryo

abnormal gastrulation ( J:157256 )

• at E7.5 formation of the germ layers appears impaired

abnormal germ layer development ( J:157256 )

• formation or morphogenesis of germ layers is impaired resulting in an endodermal layer surrounding a disorganized mass of cells

abnormal mesoderm development ( J:157256 )

• lack of epithelial morphology, without a transition to mesodermal fate based upon E-cadherin or N-cadherin expression

abnormal proximal-distal axis patterning ( J:157256 )

• at 6.5 rounder appearance of the embyros suggests decreased elongation of the proximodistal axis

abnormal rostral-caudal axis patterning ( J:157256 )

• unable to identify a clear AP axis, even at 8.5 dpc

abnormal embryonic epiblast morphology ( J:157256 )

• at E6.5 cells within the epiblast are less well organized

• at E7.5 and later cells of the epiblast fail to display the characteristic columnar epithelial morphology and the embryo has not formed a distinct embryonic cavity

decreased embryonic epiblast cell proliferation ( J:157256 )

• at E7.5 proliferation rate is decreased in the epiblast

abnormal primitive streak elongation ( J:157256 )

• immunofluorescence and immunohistochemical studies suggest AP axis has been specified, and the primitive streak has been induced, but fails to elongate

cellular

decreased embryonic epiblast cell proliferation ( J:157256 )

• at E7.5 proliferation rate is decreased in the epiblast