Analysis Tools|
Allele Symbol Allele Name Allele ID |
Braftm1Sva targeted mutation 1, Alcino J Silva MGI:3620008 |
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| Summary |
12 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• primary mouse embryonic fibroblasts exposed to 4OHT for 5 days to activate cre/ERT2 recombinase exhibit decreased proliferation that is similar to that in single Kras mutants without further additive effects
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 30 day old mice fed a tamoxifen diet for 3 months are healthy and show normal weight and activity and no obvious anatomical defects
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• cultured DRG neurons transfected with Cre-EGFP have very short axons, compared to long and dense axon networks formed by control neurons
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• cultured DRG neurons transfected with Cre-EGFP have very short axons, compared to long and dense axon networks formed by control neurons
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• no double conditional embryos are found live at E14-15, but can be isolated at E13
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• DRG neurons cultured with NGF for 5 days show impaired axon outgrowth
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• sensory nerve trunks form normally, but distal arborization is reduced compared to controls
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• at E13, Ret levels in DRGs are reduced
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• DRG neurons cultured with NGF for 5 days show impaired axon outgrowth
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• most die before weaning
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• mice are smaller in the postnatal period than Braf conditional knockouts
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• deficiency of terminal axonal projections of parvalbumin-positive neurons toward the lateral motor pools of the spinal cord is seen compared to controls
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• proprioceptive axons enter spinal cord normally, but few progress beyond intermediate zone
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• at P12, number of Ret+ neurons in DRG is reduced; numbers of CGRG+ neurons are increased
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice show no phenotypic abnormalities at any stage
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• although born alive death occurs around 21 days of age from aggressive neurodegenerative disease
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• after being born alive, pups show progressive growth retardation
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• six month old mice have poor context discrimination although contextual conditioning is normal
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• six month old mice much slower to learn the platform position in a hidden platform Morris water maze (Motor coordination normal)
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• six month old mice are deficient in the early phase of long term potentiation although there is no disruption of synaptic transmission or presynaptic plasticity
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• following intratracheal instillation of adenovirus expressing Cre-recombinase (Ad-Cre), mice show 50% survival at 41.5 weeks compared to 40 weeks in single Kras heterozygous controls, indicating similar survival
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• mice develop non-small cell lung carcinoma following intratracheal instillation of adenovirus expressing Cre-recombinase (Ad-Cre); tumor burden in similar to that seen in single Kras heterozygotes
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• mice develop non-small cell lung carcinoma following intratracheal instillation of adenovirus expressing Cre-recombinase (Ad-Cre); tumor burden in similar to that seen in single Kras heterozygotes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• primary mouse embryonic fibroblasts exposed to 4OHT for 5 days to activate cre/ERT2 recombinase exhibit decreased proliferation compared to single Kras heterozygotes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice tend to die in third week postnatal, although some survive to P35
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• at P32, body weight is ~25% of littermates
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• from P12-14 onwards, mice show severe growth retardation
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| N |
• mice show normal nociceptive response in hotplate assay
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• mice appear hyperactive
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| N |
• dorsal root ganglion neurons survive in mutants
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• perivascular hypothalamic axonal innervation is reduced
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• anterior lobe of pituitary gland is markedly reduced in size compared to controls
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• disproportionate thinning of cortex is observed
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• perivascular hypothalamic axonal innervation is reduced
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• anterior lobe of pituitary gland is markedly reduced in size compared to controls
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• mice display significantly reduced bolood glucose levels
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• when separated from other mice, body temperature drops rapidly
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• level is elevated in pituitary relative to controls
• level of growth hormone is elevated in individual pituitary endocrine cells
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/19/2024 MGI 6.24 |
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