Phenotypes associated with this allele

• Allele Symbol: Akap1^tm1Gsm
• Allele Name: targeted mutation 1, G Stanley McKnight
• Allele ID: MGI:3620561
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Akap1^tm1Gsm/Akap1^tm1Gsm	B6.Cg-Akap1^tm1Gsm	MGI:3621191

Genotype
MGI:3621191

hm1

• Allelic Composition: Akap1^tm1Gsm/Akap1^tm1Gsm
• Genetic Background: B6.Cg-Akap1^tm1Gsm

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

reproductive system

abnormal female meiosis ( J:106320 )

♀ • after superovulation many oocytes fail to progress to metaphase II and are either degenerated or arrested at the germinal vesicle stage

♀ • after 3 hours in culture only 50% of oocytes progress from the germinal vesicle stage to germinal vesicle breakdown compared to 79% of heterozygous oocytes

♀ • addition of 100 uM Rp-adenosine 3,5-cyclic monophosphorothioate rescues maturation of homozygous oocytes

♀ • homozygous oocytes are more sensitive to inhibition of germinal vesicle breakdown by phosphodiesterase inhibitors or cAMP analogs

reduced female fertility ( J:106320 )

♀ • in crosses to wild-type males homozygous females have a birth rate of 0.26 litters/female/month and a litter size of 2.2 +/- 0.8 pups/litter compared to 0.86 litters/female/month and a litter size of 7.7 +/- 1.2 pups/litter for heterozygous females

decreased litter size ( J:106320 )

• in crosses to wild-type males homozygous females have a litter size of 2.2 +/- 0.8 pups/litter compared 7.7 +/- 1.2 pups/litter for heterozygous females

cellular

abnormal female meiosis ( J:106320 )

♀ • after superovulation many oocytes fail to progress to metaphase II and are either degenerated or arrested at the germinal vesicle stage

♀ • after 3 hours in culture only 50% of oocytes progress from the germinal vesicle stage to germinal vesicle breakdown compared to 79% of heterozygous oocytes

♀ • addition of 100 uM Rp-adenosine 3,5-cyclic monophosphorothioate rescues maturation of homozygous oocytes

♀ • homozygous oocytes are more sensitive to inhibition of germinal vesicle breakdown by phosphodiesterase inhibitors or cAMP analogs