Phenotypes associated with this allele

• Allele Symbol: Rr149356^tm1Che
• Allele Name: targeted mutation 1, Jianzhu Chen
• Allele ID: MGI:3620665
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Rr149356^tm1Che/Rr149356^tm1Che	involves: 129S4/SvJae * C57BL/6	MGI:3620759
cx2	Rr149356^tm1Che/Rr149356^tm1Che Tcrd^tm1Mom/Tcrd^tm1Mom Trp53^tm1Tyj/Trp53^tm1Tyj	involves: 129P2/OlaHsd * 129S2/SvPas * 129S4/SvJae * C57BL/6	MGI:3620761
cx3	Rr149356^tm1Che/Rr149356^tm1Che Trp53^tm1Tyj/Trp53^tm1Tyj	involves: 129S2/SvPas * 129S4/SvJae * C57BL/6	MGI:3620760

Genotype
MGI:3620759

hm1

• Allelic Composition: Rr149356^tm1Che/Rr149356^tm1Che
• Genetic Background: involves: 129S4/SvJae * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal double-negative T cell morphology ( J:106246 , J:107306 )

• thymocyte development largely blocked at DN3 stage (J:106246)

• at 4-6 weeks of age, 60% of double negative thymocytes are apoptotic (J:106246)

• Tcr Dbeta1-Jbeta1 rearrangements not detected (J:107306)

• Tcr Dbeta2-Jbeta2 found but at 25 fold less than normal levels (J:107306)

• severely reduced Rag mediated cleavages at Dbeta or Jbeta (J:107306)

abnormal double-positive T cell morphology ( J:106246 , J:107306 )

• at 4-6 weeks of age, 80% of double positive thymocytes are apoptotic (J:106246)

• Tcr Dbeta1-Jbeta1 rearrangements not detected (J:107306)

• Tcr Dbeta2-Jbeta2 found but at 25 fold less than normal levels (J:107306)

• Tcr Dbeta1-Jbeta2 rearrangements seen but at 1% of normal levels (J:107306)

• Tcr Dbeta1-Jbeta2 usually retained at least partial Dbeta2 sequence, only 21% were normal (J:107306)

neoplasm

neoplasm ( J:106246 )

N • no thymic neoplasms during first year

hematopoietic system

abnormal double-negative T cell morphology ( J:106246 , J:107306 )

• thymocyte development largely blocked at DN3 stage (J:106246)

• at 4-6 weeks of age, 60% of double negative thymocytes are apoptotic (J:106246)

• Tcr Dbeta1-Jbeta1 rearrangements not detected (J:107306)

• Tcr Dbeta2-Jbeta2 found but at 25 fold less than normal levels (J:107306)

• severely reduced Rag mediated cleavages at Dbeta or Jbeta (J:107306)

abnormal double-positive T cell morphology ( J:106246 , J:107306 )

• at 4-6 weeks of age, 80% of double positive thymocytes are apoptotic (J:106246)

• Tcr Dbeta1-Jbeta1 rearrangements not detected (J:107306)

• Tcr Dbeta2-Jbeta2 found but at 25 fold less than normal levels (J:107306)

• Tcr Dbeta1-Jbeta2 rearrangements seen but at 1% of normal levels (J:107306)

• Tcr Dbeta1-Jbeta2 usually retained at least partial Dbeta2 sequence, only 21% were normal (J:107306)

Genotype
MGI:3620761

cx2

• Allelic Composition: Rr149356^tm1Che/Rr149356^tm1Che; Tcrd^tm1Mom/Tcrd^tm1Mom; Trp53^tm1Tyj/Trp53^tm1Tyj
• Genetic Background: involves: 129P2/OlaHsd * 129S2/SvPas * 129S4/SvJae * C57BL/6

immune system

increased T cell derived lymphoma incidence ( J:106246 )

• most thymocytes are double negative at 4 weeks of age but quickly progress to double positive and then CD8 single positive lymphomas

• mean survival around 14 weeks

abnormal double-negative T cell morphology ( J:106246 )

• most thymocytes are double negative at 4 weeks of age but quickly progress to double positive and then CD8 single positive lymphomas

neoplasm

increased T cell derived lymphoma incidence ( J:106246 )

• most thymocytes are double negative at 4 weeks of age but quickly progress to double positive and then CD8 single positive lymphomas

• mean survival around 14 weeks

hematopoietic system

increased T cell derived lymphoma incidence ( J:106246 )

• most thymocytes are double negative at 4 weeks of age but quickly progress to double positive and then CD8 single positive lymphomas

• mean survival around 14 weeks

abnormal double-negative T cell morphology ( J:106246 )

• most thymocytes are double negative at 4 weeks of age but quickly progress to double positive and then CD8 single positive lymphomas

endocrine/exocrine glands

increased T cell derived lymphoma incidence ( J:106246 )

• most thymocytes are double negative at 4 weeks of age but quickly progress to double positive and then CD8 single positive lymphomas

• mean survival around 14 weeks

Genotype
MGI:3620760

cx3

• Allelic Composition: Rr149356^tm1Che/Rr149356^tm1Che; Trp53^tm1Tyj/Trp53^tm1Tyj
• Genetic Background: involves: 129S2/SvPas * 129S4/SvJae * C57BL/6

immune system

increased T cell derived lymphoma incidence ( J:106246 )

• 2/3 of mice develop thymic lymphomas

• single positive Cd8 cells are immature and abnormal, make up 60% of thymocytes by 8 weeks

• large terminal lymphomas present by 11-17 weeks of age

• mean survival of 14 weeks

abnormal double-negative T cell morphology ( J:106246 )

• 24% of double negative thymocytes are apoptotic

abnormal double-positive T cell morphology ( J:106246 )

• 36% of double positive thymocytes are apoptotic

• double positive cells start losing CD4 expression around 7 weeks to become CD8 single positive

neoplasm

increased T cell derived lymphoma incidence ( J:106246 )

• 2/3 of mice develop thymic lymphomas

• single positive Cd8 cells are immature and abnormal, make up 60% of thymocytes by 8 weeks

• large terminal lymphomas present by 11-17 weeks of age

• mean survival of 14 weeks

decreased metastatic potential ( J:106246 )

• inefficient metastasis

hematopoietic system

increased T cell derived lymphoma incidence ( J:106246 )

• 2/3 of mice develop thymic lymphomas

• single positive Cd8 cells are immature and abnormal, make up 60% of thymocytes by 8 weeks

• large terminal lymphomas present by 11-17 weeks of age

• mean survival of 14 weeks

abnormal double-negative T cell morphology ( J:106246 )

• 24% of double negative thymocytes are apoptotic

abnormal double-positive T cell morphology ( J:106246 )

• 36% of double positive thymocytes are apoptotic

• double positive cells start losing CD4 expression around 7 weeks to become CD8 single positive

endocrine/exocrine glands

increased T cell derived lymphoma incidence ( J:106246 )

• 2/3 of mice develop thymic lymphomas

• single positive Cd8 cells are immature and abnormal, make up 60% of thymocytes by 8 weeks

• large terminal lymphomas present by 11-17 weeks of age

• mean survival of 14 weeks