Phenotypes associated with this allele

• Allele Symbol: Tg(Scgb1a1-cre)1Kkw
• Allele Name: transgene insertion 1, K Wong
• Allele ID: MGI:3623212
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Kras^tm4Tyj/Kras^+ Tg(Scgb1a1-cre)1Kkw/?	involves: 129 * C57BL/6 * FVB/N	MGI:3624415
cn2	Sox2^tm1.1Lan/Sox2^tm1.1Lan Tg(Scgb1a1-cre)1Kkw/0	involves: 129/Sv * C57BL/6	MGI:4430214

Genotype
MGI:3624415

cn1

• Allelic Composition: Kras^tm4Tyj/Kras⁺; Tg(Scgb1a1-cre)1Kkw/?
• Genetic Background: involves: 129 * C57BL/6 * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:107260 )

• early mortality with a median survival of 8 weeks

neoplasm

increased lung adenocarcinoma incidence ( J:107260 )

• progressive phenotype characterized by cellular atypia, adenoma, and ultimately adenocarcinoma in CC10 positive cells of bronchial epithelia

immune system

increased inflammatory response ( J:107260 )

• a robust inflammatory response characterized by an abundant infiltration of alveolar macrophages and neutrophils

respiratory system

increased lung adenocarcinoma incidence ( J:107260 )

• progressive phenotype characterized by cellular atypia, adenoma, and ultimately adenocarcinoma in CC10 positive cells of bronchial epithelia

Genotype
MGI:4430214

cn2

• Allelic Composition: Sox2^tm1.1Lan/Sox2^tm1.1Lan; Tg(Scgb1a1-cre)1Kkw/0
• Genetic Background: involves: 129/Sv * C57BL/6

Sox2^tm1.1Lan/Sox2^tm1.1Lan Tg(Scgb1a1-cre)1Kkw/0 mice show cuboidal-to-squamous epithelium lacking goblet, Clara and ciliated cells in the conducting airway epithelium

respiratory system

absent respiratory motile cilia ( J:155963 )

• at 4 weeks of age, mice lack the apical cilia characteristic of ciliated cells in large bronchioles and small bronchioles

abnormal lung epithelium morphology ( J:155963 )

• during the first 4 weeks of life, mice show a progressive and extensive loss of normal conducting airway epithelial cells

• however, no pulmonary inflammation is observed and mice survive normally for >6 months

abnormal bronchiole epithelium morphology ( J:155963 )

• at 4 weeks of age, a single layer of low cuboidal-to-squamous epithelium lacking the ''domed'' morphology characteristic of Clara cells, the apical cilia characteristic of ciliated cells, and goblet shape of mucus producing cells, is present in large bronchioles and small bronchioles

• at 4 weeks of age, the proliferation index of bronchiolar cells is reduced by 42% relative to that in wild-type lungs

• cell width increases 13%, cell height decreases 33%, and cell density decreases 12%, consistent with a shift from columnar to cuboidal-squamous morphology

• by 6 weeks of age, few ciliated cells remain in the bronchioles

absent club cells ( J:155963 )

• no Clara cells are identified at birth and thereafter

• loss of Clara cells occurs faster than loss of ciliated cells

abnormal respiratory mucosa goblet cell morphology ( J:155963 )

• no allergen-induced goblet cell differentiation or mucus production is observed in the bronchiolar epithelium

absent respiratory mucosa goblet cells ( J:155963 )

• at 4 weeks of age, mice lack goblet cells

• goblets cells fail to form following allergen exposure

cellular

absent respiratory motile cilia ( J:155963 )

• at 4 weeks of age, mice lack the apical cilia characteristic of ciliated cells in large bronchioles and small bronchioles