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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Prdm9tm1Ymat
targeted mutation 1, Yasuhisa Matsui
MGI:3623909
Summary 9 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Prdm9tm1Ymat/Prdm9tm1Ymat B6.129P2-Prdm9tm1Ymat MGI:6501042
hm2
 		Prdm9tm1Ymat/Prdm9tm1Ymat B6.Cg-Prdm9tm1Ymat MGI:6506420
hm3
 		Prdm9tm1Ymat/Prdm9tm1Ymat C3.129P2(B6)-Prdm9tm1Ymat/HandJ MGI:6490650
hm4
 		Prdm9tm1Ymat/Prdm9tm1Ymat CAST.129P2(B6)-Prdm9tm1Ymat/Hand MGI:6501007
hm5
 		Prdm9tm1Ymat/Prdm9tm1Ymat involves: 129P2/OlaHsd * C57BL/6 MGI:3624989
cx6
 		Chek2tm1b(EUCOMM)Hmgu/Chek2tm1b(EUCOMM)Hmgu
Prdm9tm1Ymat/Prdm9tm1Ymat 		B6.Cg-Chek2tm1b(EUCOMM)Hmgu Prdm9tm1Ymat MGI:6501019
cx7
 		Prdm9tm1Ymat/Prdm9tm1Ymat
Rad21ltm1b(KOMP)Wtsi/Rad21ltm1b(KOMP)Wtsi 		B6.Cg-Prdm9tm1Ymat Rad21ltm1b(KOMP)Wtsi MGI:6506404
cx8
 		Prdm9tm1Ymat/Prdm9tm1Ymat
Rec8tm1b(KOMP)Wtsi/Rec8tm1b(KOMP)Wtsi 		B6.Cg-Prdm9tm1Ymat Rec8tm1b(KOMP)Wtsi MGI:6506408
cx9
 		Prdm9tm1Ymat/Prdm9tm1Ymat
Stag3tm1e.1(KOMP)Wtsi/Stag3tm1e.1(KOMP)Wtsi 		B6.Cg-Prdm9tm1Ymat Stag3tm1e.1(KOMP)Wtsi MGI:6506415


Genotype
MGI:6501042
hm1
Allelic
Composition		 Prdm9tm1Ymat/Prdm9tm1Ymat
Genetic
Background		 B6.129P2-Prdm9tm1Ymat
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prdm9tm1Ymat mutation (3 available); any Prdm9 mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal chiasmata formation ( J:299008 )
• approximately 4% of P0 metaphase oocytes have a full complement of crossovers
abnormal chromosomal synapsis ( J:296124 )
• >98% of pachytene cells fail to properly synapse at least one pair of chromosomes
• 63.2% of (pseudo)pachytene cells show mispairing of non-homologous chromosomes in a branched 'tangled' structure
abnormal female meiosis I arrest ( J:299008 )
• P0 oocytes have widespread asynapsis
female infertility ( J:299008 )
• females fail to reproduce, but when Chek2 is also absent then some fertility is recovered

cellular
abnormal chiasmata formation ( J:299008 )
• approximately 4% of P0 metaphase oocytes have a full complement of crossovers
abnormal chromosomal synapsis ( J:296124 )
• >98% of pachytene cells fail to properly synapse at least one pair of chromosomes
• 63.2% of (pseudo)pachytene cells show mispairing of non-homologous chromosomes in a branched 'tangled' structure
abnormal female meiosis I arrest ( J:299008 )
• P0 oocytes have widespread asynapsis
abnormal double-strand DNA break repair ( J:296124 )
• increased DMC1 foci count from zygotene onwards indicating delayed repair of DSBs
• increased number of RAD51 foci in (pseudo)-pachytene cells

homeostasis/metabolism
abnormal double-strand DNA break repair ( J:296124 )
• increased DMC1 foci count from zygotene onwards indicating delayed repair of DSBs
• increased number of RAD51 foci in (pseudo)-pachytene cells




Genotype
MGI:6506420
hm2
Allelic
Composition		 Prdm9tm1Ymat/Prdm9tm1Ymat
Genetic
Background		 B6.Cg-Prdm9tm1Ymat
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prdm9tm1Ymat mutation (3 available); any Prdm9 mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
cellular phenotype ( J:280261 )
N
• normal number of double-strand DNA breaks in spermatocytes

reproductive system
reproductive system phenotype ( J:280261 )
N
• normal number of recombination hotspots in leptotene-like spermatocytes




Genotype
MGI:6490650
hm3
Allelic
Composition		 Prdm9tm1Ymat/Prdm9tm1Ymat
Genetic
Background		 C3.129P2(B6)-Prdm9tm1Ymat/HandJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prdm9tm1Ymat mutation (3 available); any Prdm9 mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
arrest of male meiosis ( J:299008 )
• meiotic arrest results in male sterility

reproductive system
arrest of male meiosis ( J:299008 )
• meiotic arrest results in male sterility




Genotype
MGI:6501007
hm4
Allelic
Composition		 Prdm9tm1Ymat/Prdm9tm1Ymat
Genetic
Background		 CAST.129P2(B6)-Prdm9tm1Ymat/Hand
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prdm9tm1Ymat mutation (3 available); any Prdm9 mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
reproductive system phenotype ( J:299008 )
N
• Background Sensitivity: Unlike homozygotes on other genetic backgrounds, homozygotes on the CAST/EiJ background have oocytes that survive, have normal fertility and produce normal litter sizes
male infertility ( J:299008 )
• Background Sensitivity: as with male homozygotes on other genetic backgrounds, male homozygotes on the CAST/EiJ genetic background exhibit meiotic arrest and are infertile, but this is a less severe phenotype on this genetic background because some round spermatids and elongating spermatids are found




Genotype
MGI:3624989
hm5
Allelic
Composition		 Prdm9tm1Ymat/Prdm9tm1Ymat
Genetic
Background		 involves: 129P2/OlaHsd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prdm9tm1Ymat mutation (3 available); any Prdm9 mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal ovarian folliculogenesis ( J:102875 )
• at 5 weeks of age no growing follicles at any stage of development are seen
decreased testis weight ( J:102875 )
• at 8 weeks of age, testis weigh 75% less than in wild-type mice
decreased oocyte number ( J:102875 )
• from E17.5 the number of germ cells is decreased with few present in neonates
abnormal chromosomal synapsis ( J:102875 )
• deficient pairing of homologous chromosomes
abnormal female meiosis ( J:102875 )
• meitotic arrest with impaired homologous chromosome pairing and sex body formation
• pachytene chromosomes are frequently branched and connected to other chromosomes
arrest of male meiosis ( J:102875 )
• meitotic arrest with impaired homologous chromosome pairing and sex body formation
• pachytene chromosomes are frequently branched and connected to other chromosomes

endocrine/exocrine glands
abnormal ovarian folliculogenesis ( J:102875 )
• at 5 weeks of age no growing follicles at any stage of development are seen
decreased testis weight ( J:102875 )
• at 8 weeks of age, testis weigh 75% less than in wild-type mice

cellular
decreased oocyte number ( J:102875 )
• from E17.5 the number of germ cells is decreased with few present in neonates
abnormal chromosomal synapsis ( J:102875 )
• deficient pairing of homologous chromosomes
abnormal female meiosis ( J:102875 )
• meitotic arrest with impaired homologous chromosome pairing and sex body formation
• pachytene chromosomes are frequently branched and connected to other chromosomes
arrest of male meiosis ( J:102875 )
• meitotic arrest with impaired homologous chromosome pairing and sex body formation
• pachytene chromosomes are frequently branched and connected to other chromosomes
abnormal double-strand DNA break repair ( J:102875 )
• germ cells show impaired double strand break repair compared to wild-type

homeostasis/metabolism
abnormal double-strand DNA break repair ( J:102875 )
• germ cells show impaired double strand break repair compared to wild-type




Genotype
MGI:6501019
cx6
Allelic
Composition		 Chek2tm1b(EUCOMM)Hmgu/Chek2tm1b(EUCOMM)Hmgu
Prdm9tm1Ymat/Prdm9tm1Ymat
Genetic
Background		 B6.Cg-Chek2tm1b(EUCOMM)Hmgu Prdm9tm1Ymat
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chek2tm1b(EUCOMM)Hmgu mutation (1 available); any Chek2 mutation (54 available)
Prdm9tm1Ymat mutation (3 available); any Prdm9 mutation (46 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal chiasmata formation ( J:299008 )
• although there are fewer than normal metaphase oocytes with normal chiasmata between homologous chromosomes, there is a 25% increase compared to mice with wild-type CHEK2
abnormal female meiosis I arrest ( J:299008 )
• P0 oocytes show asynapsis, but to a much less degree than in the presence of CHEK2 and a comparative 3 fold increase in dictyate oocytes

reproductive system
abnormal chiasmata formation ( J:299008 )
• although there are fewer than normal metaphase oocytes with normal chiasmata between homologous chromosomes, there is a 25% increase compared to mice with wild-type CHEK2
abnormal female meiosis I arrest ( J:299008 )
• P0 oocytes show asynapsis, but to a much less degree than in the presence of CHEK2 and a comparative 3 fold increase in dictyate oocytes
reduced female fertility ( J:299008 )
• although there is reduced fertility compared with C57BL/6J controls, compared with Prdm9 null mice wild-type for CHEK2 these double null females produce at least 1 litter in 6 months so have comparatively increased fertility, yet have a high rate of spontaneous abortion and perinatal deaths in pups.




Genotype
MGI:6506404
cx7
Allelic
Composition		 Prdm9tm1Ymat/Prdm9tm1Ymat
Rad21ltm1b(KOMP)Wtsi/Rad21ltm1b(KOMP)Wtsi
Genetic
Background		 B6.Cg-Prdm9tm1Ymat Rad21ltm1b(KOMP)Wtsi
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prdm9tm1Ymat mutation (3 available); any Prdm9 mutation (46 available)
Rad21ltm1b(KOMP)Wtsi mutation (1 available); any Rad21l mutation (34 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
cellular phenotype ( J:280261 )
N
• normal number of double-strand DNA breaks in spermatocytes




Genotype
MGI:6506408
cx8
Allelic
Composition		 Prdm9tm1Ymat/Prdm9tm1Ymat
Rec8tm1b(KOMP)Wtsi/Rec8tm1b(KOMP)Wtsi
Genetic
Background		 B6.Cg-Prdm9tm1Ymat Rec8tm1b(KOMP)Wtsi
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prdm9tm1Ymat mutation (3 available); any Prdm9 mutation (46 available)
Rec8tm1b(KOMP)Wtsi mutation (1 available); any Rec8 mutation (25 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal double-strand DNA break repair ( J:280261 )
• reduced number of double-strand DNA breaks (60-80% of WT or single KO) in spermatocytes

homeostasis/metabolism
abnormal double-strand DNA break repair ( J:280261 )
• reduced number of double-strand DNA breaks (60-80% of WT or single KO) in spermatocytes




Genotype
MGI:6506415
cx9
Allelic
Composition		 Prdm9tm1Ymat/Prdm9tm1Ymat
Stag3tm1e.1(KOMP)Wtsi/Stag3tm1e.1(KOMP)Wtsi
Genetic
Background		 B6.Cg-Prdm9tm1Ymat Stag3tm1e.1(KOMP)Wtsi
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prdm9tm1Ymat mutation (3 available); any Prdm9 mutation (46 available)
Stag3tm1e.1(KOMP)Wtsi mutation (1 available); any Stag3 mutation (54 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
reproductive system
abnormal male meiosis ( J:280261 )
• reduced number (30% of WT, 27-56% of either single KO) of recombination hotspots in leptotene-like spermatocytes

cellular
abnormal male meiosis ( J:280261 )
• reduced number (30% of WT, 27-56% of either single KO) of recombination hotspots in leptotene-like spermatocytes
abnormal double-strand DNA break repair ( J:280261 )
• reduced number of double-strand DNA breaks (20-30% of WT or either single KO) in spermatocytes

homeostasis/metabolism
abnormal double-strand DNA break repair ( J:280261 )
• reduced number of double-strand DNA breaks (20-30% of WT or either single KO) in spermatocytes




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Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory