Phenotypes associated with this allele

• Allele Symbol: Espl1^tm1.2Tno
• Allele Name: targeted mutation 1.2 Tetsuo Noda
• Allele ID: MGI:3624003
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Espl1^tm1.2Tno/Espl1^tm1.2Tno	involves: 129S4/SvJae * C57BL/6J	MGI:3714321
cn2	Espl1^tm1.1Tno/Espl1^tm1.2Tno	involves: 129S4/SvJae * C57BL/6J	MGI:3714319
cx3	Espl1^tm1.2Tno/Espl1^+ Pttg1^tm1.1Tno/Pttg1^tm1.1Tno	involves: 129S4/SvJae * C57BL/6J	MGI:3714317

Genotype
MGI:3714321

hm1

• Allelic Composition: Espl1^tm1.2Tno/Espl1^tm1.2Tno
• Genetic Background: involves: 129S4/SvJae * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:107917 )

• although homozygous blastocysts are present at E3.5, no homozygous embryos are recovered at E8.5

embryo

abnormal inner cell mass proliferation ( J:107917 )

• trophoblasts do not spread in a E3.5 embryo after day 3 in culture

abnormal blastocyst morphology ( J:107917 )

• E3.5 embryos after day 3 in culture fail to either expand the inner cell mass and trophoblasts do not spread

• blastocyst are smaller than normal

• E3.5 embryos after day 1 in culture have 10% of the cells in a normal blastocyst and have enlarged nuclei by 2 times the normal diameter

cellular

abnormal cell nucleus morphology ( J:107917 )

• E3.5 embryos after day 1 in culture have enlarged nuclei by 2 times the normal diameter

abnormal inner cell mass proliferation ( J:107917 )

• trophoblasts do not spread in a E3.5 embryo after day 3 in culture

Genotype
MGI:3714319

cn2

• Allelic Composition: Espl1^tm1.1Tno/Espl1^tm1.2Tno
• Genetic Background: involves: 129S4/SvJae * C57BL/6J

cellular

abnormal cell nucleus morphology ( J:107917 )

• following treatment with Cre-adenovirus, mouse embryonic fibroblasts (MEFs) have enlarged nuclei

decreased cell proliferation ( J:107917 )

• following treatment with Cre-adenovirus, mouse embryonic fibroblasts (MEFs) exhibit growth retardation

• DNA replication continues to occur

• however, infection with Cre-adenovirus can reduce some growth in the absence of floxed alleles

Genotype
MGI:3714317

cx3

• Allelic Composition: Espl1^tm1.2Tno/Espl1⁺; Pttg1^tm1.1Tno/Pttg1^tm1.1Tno
• Genetic Background: involves: 129S4/SvJae * C57BL/6J

mortality/aging

embryonic lethality, complete penetrance ( J:107917 )

• pups die prior to E12.5

growth/size/body

embryonic growth retardation ( J:107917 )

embryo

embryonic growth retardation ( J:107917 )