Phenotypes associated with this allele

• Allele Symbol: Espl1^tm1.1Kna
• Allele Name: targeted mutation 1.1, Kim Nasmyth
• Allele ID: MGI:3624200
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Espl1^tm1.1Kna/Espl1^tm1.2Kna Wapl^tm1.1Jmpt/Wapl^tm1.2Jmpt Gt(ROSA)26Sor^tm9(cre/ESR1)Arte/Gt(ROSA)26Sor^+	involves: 129P2/OlaHsd * C57BL/6 * C57BL/6NTac * SJL	MGI:5551815
cn2	Espl1^tm1.1Kna/Espl1^tm1.1Kna Tg(Mx1-cre)1Cgn/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3625271

Genotype
MGI:5551815

cn1

• Allelic Composition: Espl1^tm1.1Kna/Espl1^tm1.2Kna; Wapl^tm1.1Jmpt/Wapl^tm1.2Jmpt; Gt(ROSA)26Sor^{tm9(cre/ESR1)Arte}/Gt(ROSA)26Sor⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * C57BL/6NTac * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal mitosis ( J:205429 )

• tamoxifen-treated mouse embryonic fibroblasts exhibit diplo-chromosomes with unseparated arms unlike in control cells

Genotype
MGI:3625271

cn2

• Allelic Composition: Espl1^tm1.1Kna/Espl1^tm1.1Kna; Tg(Mx1-cre)1Cgn/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

liver/biliary system

abnormal hepatocyte morphology ( J:107916 )

• in cultured hepatocytes treated with poly(I)poly(C) 80% of anaphase cells have sister chromatids that fail to properly disjoin so that mitosis produces cells with micronuclei and abnormally large nuclei

abnormal liver regeneration ( J:107916 )

• after a injection with 400 ul poly(I)poly(C) and a two thirds hepatectomy, liver regeneration occurs but cells are larger, contain larger nuclei, and increased DNA content (many cell with 8C, 16C,or 32C DNA content) suggesting that regeneration occurs by genome replication without cell proliferation

hematopoietic system

decreased bone marrow cell number ( J:107916 )

• 3 days after a injection with 400 ul poly(I)poly(C) severe bone marrow aplasia is seen with only erythrocytes present; however, by 31 days after injection bone marrow cell numbers return to normal (all of these new hematopoeitic cells contain unrecombined alleles)