Phenotypes associated with this allele

• Allele Symbol: Trp53^tm3Wahl
• Allele Name: targeted mutation 3, Geoffrey M Wahl
• Allele ID: MGI:3624429
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Trp53^tm3Wahl/Trp53^tm3Wahl	involves: 129S4/SvJae	MGI:3700135
cx2	Mdm2^tm1Glo/Mdm2^+ Mdm4^Gt(VICTR20)7Lex/Mdm4^+ Trp53^tm3Wahl/Trp53^tm3Wahl	involves: 129	MGI:3700144
cx3	Mdm4^Gt(VICTR20)7Lex/Mdm4^Gt(VICTR20)7Lex Trp53^tm3Wahl/Trp53^tm3Wahl	involves: 129S4/SvJae * 129S5/SvEvBrd	MGI:3700141
cx4	Mdm2^tm1Glo/Mdm2^tm1Glo Trp53^tm3Wahl/Trp53^tm3Wahl	involves: 129S4/SvJae * 129S7/SvEvBrd	MGI:3700140
cx5	Mdm2^tm1Glo/Mdm2^+ Trp53^tm3Wahl/Trp53^tm3Wahl	involves: 129S4/SvJae * 129S7/SvEvBrd	MGI:3700143

Genotype
MGI:3700135

hm1

• Allelic Composition: Trp53^tm3Wahl/Trp53^tm3Wahl
• Genetic Background: involves: 129S4/SvJae

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

mortality/aging ( J:108183 )

N • no significant loss of homozygotes is detected at birth

cellular

abnormal cell cycle ( J:108183 , J:118244 )

• MEFs fail to arrest after irradiation or exposure to concentrations of adriamycin that induce arrest in wild-type cells (J:108183)

• MEFs fail to arrest after exposure to 6 Gy or 12 Gy irradiation (J:118244)

decreased cellular sensitivity to gamma-irradiation ( J:118244 )

• 10 Gy of whole body irradiation results in practically no thymocyte apoptosis unlike in wild-type mice where about 1/3 of thymocytes are apoptotic

• MEFs fail to arrest after exposure to 6 Gy or 12 Gy irradiation

decreased apoptosis ( J:108183 , J:118244 )

• adriamycin-induced apoptosis is significantly reduced at low (0.25 ug/ml) but not at high (0.5 and 1.0 ug/ml) concentrations of adriamycin compared to wild-type cells (J:108183)

• irradiation with 6 Gy or 12 Gy induces a smaller but still measurable level of apoptosis compared to wild-type cells (J:108183)

increased cell proliferation ( J:108183 )

• MEFs fail to undergo replicative senescence

neoplasm

increased tumor latency ( J:108183 )

• onset of tumor development is delayed compared to Trp53^tm1Brd homozygotes

Genotype
MGI:3700144

cx2

• Allelic Composition: Mdm2^tm1Glo/Mdm2⁺; Mdm4^{Gt(VICTR20)7Lex}/Mdm4⁺; Trp53^tm3Wahl/Trp53^tm3Wahl
• Genetic Background: involves: 129

cellular

abnormal cell cycle ( J:108183 )

• cell cycle arrest in irradiate MEFs is improved compared to Trp53 single homozygous MEFs but is still not as robust as in wild-type MEFs

Genotype
MGI:3700141

cx3

• Allelic Composition: Mdm4^{Gt(VICTR20)7Lex}/Mdm4^{Gt(VICTR20)7Lex}; Trp53^tm3Wahl/Trp53^tm3Wahl
• Genetic Background: involves: 129S4/SvJae * 129S5/SvEvBrd

mortality/aging

mortality/aging ( J:108183 )

N • viable double homozygous mice are found in Mendelian proportions

cellular

abnormal cell cycle ( J:108183 )

• cell cycle arrest in irradiate MEFs is improved compared to Trp53 single homozygous MEFs but is still not as robust as in wild-type MEFs

decreased fibroblast proliferation ( J:108183 )

• cell proliferation in double homozygous MEFs is reduced by about 30% compared to Trp53 single homozygous MEFs

Genotype
MGI:3700140

cx4

• Allelic Composition: Mdm2^tm1Glo/Mdm2^tm1Glo; Trp53^tm3Wahl/Trp53^tm3Wahl
• Genetic Background: involves: 129S4/SvJae * 129S7/SvEvBrd

mortality/aging

prenatal lethality, complete penetrance ( J:108183 )

• no double homozygous mice are found

Genotype
MGI:3700143

cx5

• Allelic Composition: Mdm2^tm1Glo/Mdm2⁺; Trp53^tm3Wahl/Trp53^tm3Wahl
• Genetic Background: involves: 129S4/SvJae * 129S7/SvEvBrd

cellular

abnormal cell cycle ( J:108183 )

• irradiation-induced cell cycle arrest is partially restored these MEFs compared to Trp53 single homozygous MEFs