Phenotypes associated with this allele

• Allele Symbol: Tnf^tm2.1Gkl
• Allele Name: targeted mutation 2.1, George Kollias
• Allele ID: MGI:3625620
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Cd19^tm1(cre)Cgn/Cd19^+ Tnf^tm2.1Gkl/Tnf^tm2.1Gkl	involves: 129P2/OlaHsd * 129S/SvEv * 129S6/SvEvTac * C57BL/6J	MGI:3629600
cn2	Tnf^tm2.1Gkl/Tnf^+ Lyz2^tm1(cre)Ifo/Lyz2^+	involves: 129P2/OlaHsd * 129S/SvEv * 129S6/SvEvTac * C57BL/6J	MGI:3629610
cn3	Tnf^tm2.1Gkl/Tnf^tm2.1Gkl Lyz2^tm1(cre)Ifo/Lyz2^+	involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6J	MGI:3629596
cn4	Tnf^tm2.1Gkl/Tnf^tm2.1Gkl Tg(Fabp1-cre)1Jig/0	involves: 129S/SvEv * FVB/N	MGI:5558947
cn5	Tnf^tm2.1Gkl/Tnf^tm2.1Gkl Tg(Lck-cre)I57Jxm/0	involves: 129S/SvEv * ICR	MGI:3629599

Genotype
MGI:3629600

cn1

• Allelic Composition: Cd19^tm1(cre)Cgn/Cd19⁺; Tnf^tm2.1Gkl/Tnf^tm2.1Gkl
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * 129S6/SvEvTac * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:108572 )

N • unlike mice with recombination in T cells, mice do not develop inflammatory bowel disease

Genotype
MGI:3629610

cn2

• Allelic Composition: Tnf^tm2.1Gkl/Tnf⁺; Lyz2^tm1(cre)Ifo/Lyz2⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * 129S6/SvEvTac * C57BL/6J

growth/size/body

weight loss ( J:108572 )

• past 4 months of age, mice display weight loss

Genotype
MGI:3629596

cn3

• Allelic Composition: Tnf^tm2.1Gkl/Tnf^tm2.1Gkl; Lyz2^tm1(cre)Ifo/Lyz2⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S/SvEv * C57BL/6J

growth/size/body

weight loss ( J:108572 )

• past 4 months of age, mice display weight loss which is more severe than in mice heterozygous for both alleles

Genotype
MGI:5558947

cn4

• Allelic Composition: Tnf^tm2.1Gkl/Tnf^tm2.1Gkl; Tg(Fabp1-cre)1Jig/0
• Genetic Background: involves: 129S/SvEv * FVB/N

digestive/alimentary system

abnormal intestinal mucosa morphology ( J:204557 )

• disruption of the mucosal architecture with prominent villous distortion characterized by widening and decreased number of villi

• increase in numbers of activated CD4+ cells within their intestinal mucosa

abnormal small intestinal villus morphology ( J:204557 )

• prominent villous distortion characterized by widening and decreased number of villi

decreased small intestinal villus number ( J:204557 )

large intestinal inflammation ( J:204557 )

• low-grade inflammatory cell infiltration with both active and chronic components is seen in the colon

small intestinal inflammation ( J:204557 )

• chronic small intestinal inflammation

ileum inflammation ( J:204557 )

• mice exhibit severe inflammatory changes in the ilea, including dense infiltration of the lamina propria by acute (polymorphonuclear), but mainly chronic (lymphocytes, macrophages), inflammatory cells

• however, no inflammation is seen in other parts of the small intestine, including the jejunum , and mice do not develop inflammatory arthritis

immune system

large intestinal inflammation ( J:204557 )

• low-grade inflammatory cell infiltration with both active and chronic components is seen in the colon

small intestinal inflammation ( J:204557 )

• chronic small intestinal inflammation

ileum inflammation ( J:204557 )

• mice exhibit severe inflammatory changes in the ilea, including dense infiltration of the lamina propria by acute (polymorphonuclear), but mainly chronic (lymphocytes, macrophages), inflammatory cells

• however, no inflammation is seen in other parts of the small intestine, including the jejunum , and mice do not develop inflammatory arthritis

increased circulating tumor necrosis factor level ( J:204557 )

homeostasis/metabolism

increased circulating tumor necrosis factor level ( J:204557 )

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
inflammatory bowel disease		DOID:0050589	OMIM:PS266600	J:204557

Genotype
MGI:3629599

cn5

• Allelic Composition: Tnf^tm2.1Gkl/Tnf^tm2.1Gkl; Tg(Lck-cre)I57Jxm/0
• Genetic Background: involves: 129S/SvEv * ICR

immune system

small intestinal inflammation ( J:108572 )

• mice develop mice develop inflammatory bowel disease past 5 months of age with reduced severity compared to Tnf^tm2.1Gkl, Lyzs^tm1(cre)Ifo double homozygotes or Tnf^tm2Gkl heterozygotes

digestive/alimentary system

small intestinal inflammation ( J:108572 )

• mice develop mice develop inflammatory bowel disease past 5 months of age with reduced severity compared to Tnf^tm2.1Gkl, Lyzs^tm1(cre)Ifo double homozygotes or Tnf^tm2Gkl heterozygotes