Phenotypes associated with this allele

• Allele Symbol: Pik3ca^tm1Bvan
• Allele Name: targeted mutation 1, Bart Vanhaesebroeck
• Allele ID: MGI:3628805
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pik3ca^tm1Bvan/Pik3ca^tm1Bvan	involves: 129P2/OlaHsd	MGI:3796330
hm2	Pik3ca^tm1Bvan/Pik3ca^tm1Bvan	involves: 129P2/OlaHsd * C57BL/6	MGI:3629667
ht3	Pik3ca^tm1Bvan/Pik3ca^+	involves: 129P2/OlaHsd	MGI:3796331
ht4	Pik3ca^tm1Bvan/Pik3ca^+	involves: 129P2/OlaHsd * C57BL/6	MGI:3629699

Genotype
MGI:3796330

hm1

• Allelic Composition: Pik3ca^tm1Bvan/Pik3ca^tm1Bvan
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality, complete penetrance ( J:136236 )

• mice die by E12.5

embryo

embryonic growth arrest ( J:136236 )

• at E12.5

embryonic growth retardation ( J:136236 )

• at E9.5

abnormal vitelline vascular remodeling ( J:136236 )

• primitive at E9.5

cardiovascular system

abnormal intersomitic vessel morphology ( J:136236 )

• by E10.5, the intersomitic vessels are disorganized unlike in wild-type mice

abnormal vascular development ( J:136236 )

• by E10.5, the vessels in the head and trunk are poorly remodeled and enlarged, the perineural vascular plexus is primitive and the intersomitic vessels are disorganized unlike in wild-type mice

abnormal dorsal aorta morphology ( J:136236 )

• thin at E9.5

abnormal perineural vascular plexus morphology ( J:136236 )

• primitive at E10.5

internal hemorrhage ( J:136236 )

• mice exhibit blood accumulation in the head and trunk

growth/size/body

embryonic growth retardation ( J:136236 )

• at E9.5

Genotype
MGI:3629667

hm2

• Allelic Composition: Pik3ca^tm1Bvan/Pik3ca^tm1Bvan
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:109024 )

• die around E10-E11

embryo

embryonic growth retardation ( J:109024 )

• growth-retarded from embryonic day 9

growth/size/body

embryonic growth retardation ( J:109024 )

• growth-retarded from embryonic day 9

Genotype
MGI:3796331

ht3

• Allelic Composition: Pik3ca^tm1Bvan/Pik3ca⁺
• Genetic Background: involves: 129P2/OlaHsd

cardiovascular system

abnormal retina vasculature morphology ( J:136236 )

• at P5, outgrowth of the primary vascular plexus in the retina is delayed compared to in wild-type mice

• however, endothelial cell proliferation and survival is normal

abnormal angiogenesis ( J:136236 )

• at E11.5, angiogenesis in the hindbrain is delayed compared to in wild-type mice

• at P5, outgrowth of the primary vascular plexus in the retina is delayed compared to in wild-type mice

vision/eye

abnormal retina vasculature morphology ( J:136236 )

• at P5, outgrowth of the primary vascular plexus in the retina is delayed compared to in wild-type mice

• however, endothelial cell proliferation and survival is normal

Genotype
MGI:3629699

ht4

• Allelic Composition: Pik3ca^tm1Bvan/Pik3ca⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

growth/size/body

decreased lean body mass ( J:109024 )

• lean mass determined by DEXA analysis are decreased compared to wild-type control

• major internal organ weight was not altered

decreased body weight ( J:109024 )

• throughout adult period compared to wild-type control of the same sex

decreased body length ( J:109024 )

• decreased mouth-to-anus length of 6-weeks old mice

postnatal growth retardation ( J:109024 )

• starts during embryonic development

fetal growth retardation ( J:109024 )

• reduced weight and crown-to-rump length of E18.5 embryos compared to wild-type control

homeostasis/metabolism

increased circulating insulin level ( J:109024 )

• when fasted or during a glucose tolerance test

increased circulating leptin level ( J:109024 )

♂ • significant increase in 5-week-old and 12-week-old male

impaired glucose tolerance ( J:109024 )

♀ • impaired glucose clearance during glucose tolerance test in female

♀ • fasting blood glucose levels were equivalent ot those of wild-type mice

insulin resistance ( J:109024 )

• an impaired hypoglycemic response to insulin

endocrine/exocrine glands

increased pancreatic beta cell number ( J:109024 )

behavior/neurological

polyphagia ( J:109024 )

• increased food intake in 12-week-old mice

adipose tissue

increased fat cell size ( J:109024 )

• enlarged white adipose cells

• increased fat mass and adipocyte number

muscle

decreased skeletal muscle mass ( J:109024 )

• reduced skeletal muscle mass