Phenotypes associated with this allele

• Allele Symbol: Stx1a^tm1Tfuj
• Allele Name: targeted mutation 1, Tomonori Fujiwara
• Allele ID: MGI:3629049
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Stx1a^tm1Tfuj/Stx1a^tm1Tfuj	B6.Cg-Stx1a^tm1Tfuj	MGI:5000258
hm2	Stx1a^tm1Tfuj/Stx1a^tm1Tfuj	involves: C57BL/6J	MGI:3629308
ht3	Stx1a^tm1Tfuj/Stx1a^+	B6.Cg-Stx1a^tm1Tfuj	MGI:5000259
cx4	Stx1a^tm1Tfuj/Stx1a^tm1Tfuj Stx1b^tm1Tmis/Stx1b^tm1Tmis	involves: C57BL/6J	MGI:5605505

Genotype
MGI:5000258

hm1

• Allelic Composition: Stx1a^tm1Tfuj/Stx1a^tm1Tfuj
• Genetic Background: B6.Cg-Stx1a^tm1Tfuj

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal latent inhibition of conditioning behavior ( J:171786 )

• the inhibition ratio is significantly attenuated compared to wild-type controls

• treatment with fluoxetine restores latent inhibition

abnormal response to novel object ( J:171786 )

• when presented with a novel and a familiar object exploring ration for the novel object is significantly lower than in wild-type mice

increased startle reflex ( J:171786 )

• increase in the amplitude of the acoustic startle response

• however, the habituation ratio is similar to controls

abnormal social investigation ( J:171786 )

• over multiple trials investigation time of an initially novel ovariectomized mouse declines much more slowly than in wild-type mice

nervous system

abnormal nervous system physiology ( J:171786 )

• decrease in glutamate induced serotonin release from hippocampal slices

abnormal hypothalamus secretion ( J:171786 )

• decrease in glutamate induced serotonin release from hypothalamic slices

• however, release in response to maximal stimulation with K⁺ is similar to wild-type controls

homeostasis/metabolism

increased serotonin level ( J:171786 )

• in the hypothalamus, hippocampus, and hindbrain

• however, levels of 5-HIAA, a serotonin metabolite, are similar to controls, suggesting impaired serotonin release

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
disease of mental health		DOID:150		J:171786

Genotype
MGI:3629308

hm2

• Allelic Composition: Stx1a^tm1Tfuj/Stx1a^tm1Tfuj
• Genetic Background: involves: C57BL/6J

mortality/aging

mortality/aging ( J:109042 )

N • ratio of homozygous pups found when newborn mice are genotyped is lower (19.1% of 365 mice) than expected (25%)

behavior/neurological

abnormal cued conditioning behavior ( J:109042 )

• consolidation of conditioned fear memory is impaired; 24 hours after CS-UCS pairing, freezing response to training context and to an audible cue is decreased in Stx1a-deficient mice compared to controls

• in extinction training 24 hours after acquisition of a cued fear memory, null mice show more frequent freezing responses compared to controls during each extinction trial, suggesting impairment of memory extinction

nervous system

abnormal excitatory postsynaptic potential ( J:109042 )

• theta-burst stimulation evokes a lesser increase in the slope of the field EPSP recorded from null animals compared to wild-type

reduced long-term potentiation ( J:109042 )

• in CA1-region hippocampal slices, LTP is impaired in Stx1a-deficient mice; LTP level after 60 minutes of theta-burst stimulation is 150% in null vs 187% in wild-type or 168% in heterozygotes

Genotype
MGI:5000259

ht3

• Allelic Composition: Stx1a^tm1Tfuj/Stx1a⁺
• Genetic Background: B6.Cg-Stx1a^tm1Tfuj

behavior/neurological

abnormal latent inhibition of conditioning behavior ( J:171786 )

• the inhibition ratio is significantly attenuated compared to wild-type controls

• treatment with fluoxetine restores latent inhibition

abnormal response to novel object ( J:171786 )

• when presented with a novel and a familiar object exploring ration for the novel object is significantly lower than in wild-type mice

increased startle reflex ( J:171786 )

• increase in the amplitude of the acoustic startle response

• however, the habituation ratio is similar to controls

abnormal social investigation ( J:171786 )

• over multiple trials investigation time of an initially novel ovariectomized mouse declines much more slowly than in wild-type mice

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
disease of mental health		DOID:150		J:171786

Genotype
MGI:5605505

cx4

• Allelic Composition: Stx1a^tm1Tfuj/Stx1a^tm1Tfuj; Stx1b^tm1Tmis/Stx1b^tm1Tmis
• Genetic Background: involves: C57BL/6J

mortality/aging

embryonic lethality, complete penetrance ( J:214474 )

• embryonic lethal, time not specified

nervous system

abnormal synapse morphology ( J:214474 )

• neurons show reduced and asynchronous evoked synaptic vesicle release in glutamatergic and GABAergic synapses

decreased excitatory postsynaptic current amplitude ( J:214474 )

• in E12.5 cortical cultures, the amplitude of evoked excitatory synaptic current (eEPSC) responses is smaller than in wild-type cultures and is highly asynchronous

abnormal inhibitory postsynaptic currents ( J:214474 )

• in E12.5 cortical cultures, evoked inhibitory synaptic current (eIPSc) responses are attenuated and asynchronous compared to wild-type cultures

abnormal miniature excitatory postsynaptic currents ( J:214474 )

• in E12.5 cortical cultures, the mean amplitude of miniature excitatory postsynaptic current (mEPSC) is smaller than in wild-type cultures

abnormal miniature inhibitory postsynaptic currents ( J:214474 )

• in E12.5 cortical cultures, the mean frequency and amplitude of miniature inhibitory postsynaptic current (mIPSC) are lower than in wild-type cultures

decreased miniature inhibitory postsynaptic current amplitude ( J:214474 )

• in E12.5 cortical cultures

decreased miniature inhibitory postsynaptic current frequency ( J:214474 )

• in E12.5 cortical cultures