Phenotypes associated with this allele

• Allele Symbol: Fbxo5^tm1Dlim
• Allele Name: targeted mutation 1, Dae-Sik Lim
• Allele ID: MGI:3640940
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Fbxo5^tm1Dlim/Fbxo5^tm1Dlim	involves: 129S4/SvJaeSor	MGI:3640943

Genotype
MGI:3640943

hm1

• Allelic Composition: Fbxo5^tm1Dlim/Fbxo5^tm1Dlim
• Genetic Background: involves: 129S4/SvJaeSor

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality before implantation, complete penetrance ( J:110317 )

• only completely resorbed embryos are found at E6.5 and E7.5 and blastocysts appear abnormal at E3.5

cellular

abnormal mitosis ( J:110317 )

• mitotic catastrophe with over-condensed chromosomes, and misaligned chromosomes

• cells appear to prematurely enter anaphase; however, S-phase entry and progression and mitotic entry appear normal

abnormal mitotic spindle morphology ( J:110317 )

• abnormal spindle structures at the prometaphase-like stage

• multipolar and tangled spindle structures present during anaphase

embryo

abnormal embryo development ( J:110317 )

• abnormalities are seen at the 8 and 16 cell stages

• by the blastocyst stage gross cellular degeneration is seen

absent blastocoele ( J:110317 )

• when pronuclei are collected and cultured for 4 days homozygotes fail to develop a distinct blastocele

abnormal inner cell mass morphology ( J:110317 )

• when pronuclei are collected and cultured for 4 days homozygotes fail to develop a distinct inner cell mass

• 2 homozygous embryos captured at E3.5 had severely retarded outgrowth of the inner cell mass and few of these cells survived for 5 days in culture

abnormal trophoblast layer morphology ( J:110317 )

• when pronuclei are collected and cultured for 4 days homozygotes fail to develop a distinct trophoblast