Phenotypes associated with this allele

• Allele Symbol: Jchain^tm3Tl
• Allele Name: targeted mutation 3, Tomas Leanderson
• Allele ID: MGI:3641196
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Jchain^tm3Tl/Jchain^tm3Tl	involves: 129P2/OlaHsd	MGI:3652527
ht2	Jchain^tm3Tl/Jchain^+	involves: 129P2/OlaHsd	MGI:3652528

Genotype
MGI:3652527

hm1

• Allelic Composition: Jchain^tm3Tl/Jchain^tm3Tl
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased plasma cell number ( J:49168 , J:56158 )

• the number of IgM plaque forming cells in the bone marrow and spleen are reduced (J:49168)

• following LPS-induced proliferation of spleen cells, the number of IgM plaque forming cells is reduced but overall proliferation is similar to wild-type (J:49168)

• fewer IgM producing cells are found in the lamina propria and Peyer's patches; however, the number of IgA producing cells is similar to wild-type and no change is detected in the formation of germinal centers in Peyer's patches (J:56158)

impaired complement classical pathway ( J:49168 )

• inefficient activation of complement by serum IgM

abnormal immune cell physiology ( J:49168 )

• accumulation of Ig is seen in LPS-stimulated spleen cells suggesting a defect in assembly or secretion of Ig

abnormal B cell physiology ( J:56158 )

• IgA and IgM levels are decreased in gut lavage compared to wild-type mice

• oral immunization with cholera toxin-induced gut lavage levels of IgA are reduced

increased IgA level ( J:49168 , J:56158 )

• about 4-fold (J:49168)

• baseline and oral immunization with cholera toxin-induced serum levels of IgA are increased (J:56158)

decreased IgG level ( J:49168 )

• IgG response to T-dependent antigen is reduced but not as much as the IgM response and IgG response to T-independent antigen is similar to wild-type

decreased IgM level ( J:49168 , J:56158 )

• about 4-fold (J:49168)

• high molecular mass IgM is absent from the serum indicating absence of the pentameric form of IgM that is found in wild-type mice (J:49168)

• IgM response to T-dependent or T-independent antigens is reduced (J:49168)

• baseline and oral immunization with cholera toxin-induced serum levels of IgM are decreased (J:56158)

increased susceptibility to bacterial infection ( J:56158 )

• mice orally immunized with cholera toxin display only about 20% gut antitoxic protection compared to 80% protection in immunized wild-type mice

hematopoietic system

decreased plasma cell number ( J:49168 , J:56158 )

• the number of IgM plaque forming cells in the bone marrow and spleen are reduced (J:49168)

• following LPS-induced proliferation of spleen cells, the number of IgM plaque forming cells is reduced but overall proliferation is similar to wild-type (J:49168)

• fewer IgM producing cells are found in the lamina propria and Peyer's patches; however, the number of IgA producing cells is similar to wild-type and no change is detected in the formation of germinal centers in Peyer's patches (J:56158)

abnormal B cell physiology ( J:56158 )

• IgA and IgM levels are decreased in gut lavage compared to wild-type mice

• oral immunization with cholera toxin-induced gut lavage levels of IgA are reduced

increased IgA level ( J:49168 , J:56158 )

• about 4-fold (J:49168)

• baseline and oral immunization with cholera toxin-induced serum levels of IgA are increased (J:56158)

decreased IgG level ( J:49168 )

• IgG response to T-dependent antigen is reduced but not as much as the IgM response and IgG response to T-independent antigen is similar to wild-type

decreased IgM level ( J:49168 , J:56158 )

• about 4-fold (J:49168)

• high molecular mass IgM is absent from the serum indicating absence of the pentameric form of IgM that is found in wild-type mice (J:49168)

• IgM response to T-dependent or T-independent antigens is reduced (J:49168)

• baseline and oral immunization with cholera toxin-induced serum levels of IgM are decreased (J:56158)

Genotype
MGI:3652528

ht2

• Allelic Composition: Jchain^tm3Tl/Jchain⁺
• Genetic Background: involves: 129P2/OlaHsd

immune system

decreased plasma cell number ( J:49168 )

• the number of IgM plaque forming cells in the bone marrow and spleen are reduced

• following LPS-induced proliferation of spleen cells, the number of IgM plaque forming cells is reduced but overall proliferation is similar to wild-type

impaired complement classical pathway ( J:49168 )

• inefficient activation of complement by serum IgM

abnormal immune cell physiology ( J:49168 )

• accumulation of Ig is seen in LPS-stimulated spleen cells suggesting a defect in assembly or secretion of Ig

abnormal B cell physiology ( J:56158 )

• IgA and IgM levels are decreased in gut lavage compared to wild-type mice

• oral immunization with cholera toxin-induced gut lavage levels of IgA are reduced compared to wild-type but higher than in homozygotes

increased IgA level ( J:56158 )

decreased IgM level ( J:49168 , J:56158 )

• at 12 weeks of age, but serum IgA levels are similar to wild-type (J:49168)

• high molecular mass IgM is absent from the serum indicating absence of the pentameric form of IgM that is found in wild-type mice (J:49168)

increased susceptibility to bacterial infection ( J:56158 )

• mice orally immunized with cholera toxin display reduced gut antitoxic protection compared to immunized wild-type mice but are more protected than immunized homozygotes

hematopoietic system

decreased plasma cell number ( J:49168 )

• the number of IgM plaque forming cells in the bone marrow and spleen are reduced

• following LPS-induced proliferation of spleen cells, the number of IgM plaque forming cells is reduced but overall proliferation is similar to wild-type

abnormal B cell physiology ( J:56158 )

• IgA and IgM levels are decreased in gut lavage compared to wild-type mice

• oral immunization with cholera toxin-induced gut lavage levels of IgA are reduced compared to wild-type but higher than in homozygotes

increased IgA level ( J:56158 )

decreased IgM level ( J:49168 , J:56158 )

• at 12 weeks of age, but serum IgA levels are similar to wild-type (J:49168)

• high molecular mass IgM is absent from the serum indicating absence of the pentameric form of IgM that is found in wild-type mice (J:49168)