Phenotypes associated with this allele

• Allele Symbol: Tg(Nes-cre/ERT2)5-1Imayo
• Allele Name: transgene insertion 5-1, Itaru Imayoshi
• Allele ID: MGI:3641212
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Hes1^tm1Kag/Hes1^tm1Kag Hes3^tm1Kag/Hes3^tm1Kag Hes5^tm1Fgu/Hes5^tm1Fgu Tg(Nes-cre/ERT2)5-1Imayo/0	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA	MGI:3808127
cn2	Rnf2^tm1Mvi/Rnf2^tm1Mvi Tg(Nes-cre/ERT2)5-1Imayo/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:4413349
cn3	Ezh2^tm1Yugo/Ezh2^tm1Yugo Tg(Nes-cre/ERT2)5-1Imayo/0	involves: C57BL/6	MGI:4413347
cn4	Eno2^tm1(DTA)Hsak/Eno2^tm1(DTA)Hsak Tg(Nes-cre/ERT2)5-1Imayo/0	involves: C57BL/6	MGI:3817328
cn5	Hbp1^tm1.1Ohts/Hbp1^tm1.1Ohts Tg(Nes-cre/ERT2)5-1Imayo/0	involves: C57BL/6 * CBA/JNCrlj * ICR	MGI:6361945

Genotype
MGI:3808127

cn1

• Allelic Composition: Hes1^tm1Kag/Hes1^tm1Kag; Hes3^tm1Kag/Hes3^tm1Kag; Hes5^tm1Fgu/Hes5^tm1Fgu; Tg(Nes-cre/ERT2)5-1Imayo/0
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

nervous system phenotype ( J:139170 )

N • mice exhibit no change in Cajal-Retzius cell number

Genotype
MGI:4413349

cn2

• Allelic Composition: Rnf2^tm1Mvi/Rnf2^tm1Mvi; Tg(Nes-cre/ERT2)5-1Imayo/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

nervous system

abnormal neuron differentiation ( J:154927 )

• following exposure to tamoxifen at E13.5, the late-born/upper-layer neurons in the cortical plate at P6.5 that had been labeled with BrdU at E19.0 is greater than in control Rnf2^tm1Mvi homozygotes

cellular

abnormal neuron differentiation ( J:154927 )

• following exposure to tamoxifen at E13.5, the late-born/upper-layer neurons in the cortical plate at P6.5 that had been labeled with BrdU at E19.0 is greater than in control Rnf2^tm1Mvi homozygotes

Genotype
MGI:4413347

cn3

• Allelic Composition: Ezh2^tm1Yugo/Ezh2^tm1Yugo; Tg(Nes-cre/ERT2)5-1Imayo/0
• Genetic Background: involves: C57BL/6

nervous system

abnormal neuron differentiation ( J:154927 )

• following tamoxifen treatment at E13.5 and E14.5, the number of S100beta+ cells are reduced in the neocortex at P1.5 compared to in similarly treated control mice

cellular

abnormal neuron differentiation ( J:154927 )

• following tamoxifen treatment at E13.5 and E14.5, the number of S100beta+ cells are reduced in the neocortex at P1.5 compared to in similarly treated control mice

Genotype
MGI:3817328

cn4

• Allelic Composition: Eno2^tm1(DTA)Hsak/Eno2^tm1(DTA)Hsak; Tg(Nes-cre/ERT2)5-1Imayo/0
• Genetic Background: involves: C57BL/6

nervous system

abnormal olfactory bulb morphology ( J:140993 )

• 3 weeks following treatment with tamoxifen, mice exhibit ablation of newly formed neurons in the olfactory bulb that becomes more severe at 6 and 12 weeks of treatment

decreased neuron number ( J:140993 )

• following treatment with tamoxifen, mice exhibit ablation of newly forming neurons

• following treatment with tamoxifen, neuroblasts at the entry site of the rostral migratory stream exhibit markers of apoptosis

• 3 weeks following treatment with tamoxifen, mice exhibit ablation of newly formed neurons in the olfactory bulb that becomes more severe at 6 and 12 weeks of treatment

• following treatment with tamoxifen, mice exhibit a loss of granule cells in deep regions while superficial neurons are preserved

decreased cerebellar granule cell number ( J:140993 )

• 3 weeks following treatment with tamoxifen, mice exhibit loss of granule neurons in the olfactory bulb

• following treatment with tamoxifen, mice exhibit decreased granule cell density in the dentate gyrus compared to in wild-type mice

behavior/neurological

behavior/neurological phenotype ( J:140993 )

N • despite ablation of olfactory neurons, mice exhibit normal behavior towards wild-type mice and normal odor-associated memory

Genotype
MGI:6361945

cn5

• Allelic Composition: Hbp1^tm1.1Ohts/Hbp1^tm1.1Ohts; Tg(Nes-cre/ERT2)5-1Imayo/0
• Genetic Background: involves: C57BL/6 * CBA/JNCrlj * ICR

nervous system

increased neuron apoptosis ( J:278919 )

• marked increase in Pax6+, Tbr2+ and Tuj1+ populations following tamoxifen administration at E9.5

abnormal telencephalon morphology ( J:278919 )

• telencephalic wall appears thinner following tamoxifen administration at E9.5

• telencephalic vesicles are remarkably dilated at E14.5, E16.5 and E18.5 following tamoxifen administration at E9.5

• volume of the ventral telencephalon is reduced and the wall of the dorsal telencephalon is thinner after E14.5 following tamoxifen administration at E9.5

• the ventricular surface area in the dorsolateral telencephalon (cortical region) is enlarged following tamoxifen administration at E9.5

• neuronal layer (Tuj1+) is thinner compared to controls following tamoxifen administration at E9.5

abnormal telencephalon development ( J:278919 )

• thickness of cortical neuronal layers is reduced at all stages following tamoxifen administration at E9.5

abnormal cortical ventricular zone morphology ( J:278919 )

• appears to be stretched in the tangential direction following tamoxifen administration at E9.5

decreased cortical ventricular zone thickness ( J:278919 )

• thinner following tamoxifen administration at E9.5

decreased corpus callosum size ( J:278919 )

• hypoplastic or absent at E18.5 following tamoxifen administration at E9.5

absent corpus callosum ( J:278919 )

• hypoplastic or absent at E18.5 following tamoxifen administration at E9.5

hippocampus hypoplasia ( J:278919 )

• hypoplastic or absent at E18.5 following tamoxifen administration at E9.5

absent hippocampus ( J:278919 )

• hypoplastic or absent at E18.5 following tamoxifen administration at E9.5

abnormal neuronal stem cell morphology ( J:278919 )

• increase in the number of neuronal stem cells at E14.5 following tamoxifen administration at E9.5

• rapid depletion of neuronal stem cell pool between E14.5 and E16.5 following tamoxifen administration at E9.5

decreased neuron number ( J:278919 )

• decrease in the number of Tbr2+ and early born neurons in cortical regions at E12.5 following tamoxifen administration at E9.5 indicating a delay in onset of neurogenesis

• proportional decrease in cortical neurons in all layers following tamoxifen administration at E9.5

increased neuronal stem cell self-renewal ( J:278919 )

• at E12.5 neuronal stem cells continue to expand by symmetric divisions following tamoxifen administration at E9.5

• cell cycle length is reduced in the cortex at all stages examined following tamoxifen administration at E9.5

cellular

abnormal cell cycle ( J:278919 )

• neuronal stem cell cell cycle length is reduced in the cortex at all stages examined following tamoxifen administration at E9.5

increased neuron apoptosis ( J:278919 )

• marked increase in Pax6+, Tbr2+ and Tuj1+ populations following tamoxifen administration at E9.5