Phenotypes associated with this allele

• Allele Symbol: Myd88^lkd
• Allele Name: lackadaisical
• Allele ID: MGI:3641234
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Myd88^lkd/Myd88^lkd	C57BL/6-Myd88^lkd	MGI:3641235

Genotype
MGI:3641235

hm1

• Allelic Composition: Myd88^lkd/Myd88^lkd
• Genetic Background: C57BL/6-Myd88^lkd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:110094 )

N • immunoblot analysis of lysates of homozygous mutant mouse embryonic fibroblasts (MEFs) using antibody to IkappaB demonstrates normal degradation by these cells of IkappaB in response to interleukin 1 (IL-1)

abnormal adaptive immunity ( J:110094 )

abnormal cell-mediated immunity ( J:110094 )

abnormal innate immunity ( J:110094 )

abnormal macrophage physiology ( J:110094 )

• peritoneal macrophages from homozygous mice exhibit diminished type 1 interferon secretion in response to resiquimod, a TLR7-activating ligand

• peritoneal macrophages from homozygous mice exhibit diminished type 1 interferon secretion in response to unmethylated CpG DNA, a TLR9-activating ligand

• immunoblot analysis of lysates of homozygous mutant peritoneal macrophages using antibodies to phosphorylated proteins demonstrates failure of these cells to phosphorylate/activate Ikappa B, JUN N-terminal kinase (JNK) or extracellular signal-related kinase (ERK) in response to the TLR7-activating ligand resiquimod

• immunoblot analysis of lysates of homozygous mutant peritoneal macrophages using antibodies to phosphorylated proteins demonstrates failure of these cells to phosphorylate/activate Ikappa B, JUN N-terminal kinase (JNK) or extracellular signal-related kinase (ERK) in response to the TLR1/TLR2-activating lipopeptide PAM3CSK4

hematopoietic system

abnormal macrophage physiology ( J:110094 )

• peritoneal macrophages from homozygous mice exhibit diminished type 1 interferon secretion in response to resiquimod, a TLR7-activating ligand

• peritoneal macrophages from homozygous mice exhibit diminished type 1 interferon secretion in response to unmethylated CpG DNA, a TLR9-activating ligand

• immunoblot analysis of lysates of homozygous mutant peritoneal macrophages using antibodies to phosphorylated proteins demonstrates failure of these cells to phosphorylate/activate Ikappa B, JUN N-terminal kinase (JNK) or extracellular signal-related kinase (ERK) in response to the TLR7-activating ligand resiquimod

• immunoblot analysis of lysates of homozygous mutant peritoneal macrophages using antibodies to phosphorylated proteins demonstrates failure of these cells to phosphorylate/activate Ikappa B, JUN N-terminal kinase (JNK) or extracellular signal-related kinase (ERK) in response to the TLR1/TLR2-activating lipopeptide PAM3CSK4