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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Chd4tm1.2Kge
targeted mutation 1.2, Katia Georgopoulos
MGI:3641409
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Chd4tm1.1Kge/Chd4tm1.2Kge
Tg(CAG-Bgeo/GFP)21Lbe/0
Tg(Lck-cre)1Cwi/0 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL MGI:3713384
cn2
 		Chd4tm1.2Kge/Chd4tm1.2Kge
Tg(Lck-cre)1Cwi/0 		involves: C57BL/6 * DBA/2 * SJL MGI:3653117
cn3
 		Chd4tm1.1Kge/Chd4tm1.2Kge
Tg(Lck-cre)1Cwi/0 		involves: C57BL/6 * SJL MGI:3713382


Genotype
MGI:3713384
cn1
Allelic
Composition		 Chd4tm1.1Kge/Chd4tm1.2Kge
Tg(CAG-Bgeo/GFP)21Lbe/0
Tg(Lck-cre)1Cwi/0
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chd4tm1.1Kge mutation (0 available); any Chd4 mutation (58 available)
Chd4tm1.2Kge mutation (0 available); any Chd4 mutation (58 available)
Tg(CAG-Bgeo/GFP)21Lbe mutation (2 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal cell cycle ( J:109547 )
• progressive decrease in cycling T cells is observed from second to third cell division in vitro, along with large number of nonblast T cells that appear to fail to enter cell cycle
decreased T cell proliferation ( J:109547 )
• a 5- to 7-fold decrease in proliferation is observed relative to wild-type cells in vitro after 48 hours of TCR/CD3 stimulation; fewer mutant cells are in S phase

immune system
decreased T cell proliferation ( J:109547 )
• a 5- to 7-fold decrease in proliferation is observed relative to wild-type cells in vitro after 48 hours of TCR/CD3 stimulation; fewer mutant cells are in S phase

hematopoietic system
decreased T cell proliferation ( J:109547 )
• a 5- to 7-fold decrease in proliferation is observed relative to wild-type cells in vitro after 48 hours of TCR/CD3 stimulation; fewer mutant cells are in S phase




Genotype
MGI:3653117
cn2
Allelic
Composition		 Chd4tm1.2Kge/Chd4tm1.2Kge
Tg(Lck-cre)1Cwi/0
Genetic
Background		 involves: C57BL/6 * DBA/2 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chd4tm1.2Kge mutation (0 available); any Chd4 mutation (58 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased T cell proliferation ( J:109547 )
• in vitro, TCR/CD3 stimulation shows a 5-7 fold decrease in proliferating T cells compared to controls, specifically CD8+CD4lo and CD8+ CD4- populations
thymus hypoplasia ( J:109547 )
• null mice exhibit a consistent reduction in cellularity compared to wild-type or heterozygotes
increased double-negative T cell number ( J:109547 )
• there is an increase in the population of the T cells in the late double negative (DN4) stage in the neonatal thymus
decreased double-positive T cell number ( J:109547 )
• percentage of double positive thymocytes is severely reduced
increased CD8-positive, alpha-beta T cell number ( J:109547 )
• CD8+ cells are greatly increased in number
• CD8+ populations in the thymus express TCR levels more similar to double-positive cells rather than intermediate single-positive or single-positive levels

hematopoietic system
decreased T cell proliferation ( J:109547 )
• in vitro, TCR/CD3 stimulation shows a 5-7 fold decrease in proliferating T cells compared to controls, specifically CD8+CD4lo and CD8+ CD4- populations
thymus hypoplasia ( J:109547 )
• null mice exhibit a consistent reduction in cellularity compared to wild-type or heterozygotes
increased double-negative T cell number ( J:109547 )
• there is an increase in the population of the T cells in the late double negative (DN4) stage in the neonatal thymus
decreased double-positive T cell number ( J:109547 )
• percentage of double positive thymocytes is severely reduced
increased CD8-positive, alpha-beta T cell number ( J:109547 )
• CD8+ cells are greatly increased in number
• CD8+ populations in the thymus express TCR levels more similar to double-positive cells rather than intermediate single-positive or single-positive levels

cellular
abnormal cell cycle ( J:109547 )
• activated T cells are unable to go through normal cell divisions
decreased T cell proliferation ( J:109547 )
• in vitro, TCR/CD3 stimulation shows a 5-7 fold decrease in proliferating T cells compared to controls, specifically CD8+CD4lo and CD8+ CD4- populations

endocrine/exocrine glands
thymus hypoplasia ( J:109547 )
• null mice exhibit a consistent reduction in cellularity compared to wild-type or heterozygotes




Genotype
MGI:3713382
cn3
Allelic
Composition		 Chd4tm1.1Kge/Chd4tm1.2Kge
Tg(Lck-cre)1Cwi/0
Genetic
Background		 involves: C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chd4tm1.1Kge mutation (0 available); any Chd4 mutation (58 available)
Chd4tm1.2Kge mutation (0 available); any Chd4 mutation (58 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
thymus hypoplasia ( J:109547 )
• reduced in mutant mice

immune system
thymus hypoplasia ( J:109547 )
• reduced in mutant mice

endocrine/exocrine glands
thymus hypoplasia ( J:109547 )
• reduced in mutant mice




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Send questions and comments to User Support. 		last database update
11/19/2024
MGI 6.24 		The Jackson Laboratory