Phenotypes associated with this allele

• Allele Symbol: Pdgfrb^tm1Msas
• Allele Name: targeted mutation 1, Masakiyo Sasahara
• Allele ID: MGI:3641430
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Pdgfrb^tm1Msas/Pdgfrb^tm1Msas	involves: 129 * C57BL/6	MGI:3713502
cn2	Pdgfrb^tm1Msas/Pdgfrb^tm1Msas Tg(Nes-cre)1Nogu/0	B6J.Cg-Pdgfrb^tm1Msas Tg(Nes-cre)1Nogu	MGI:5790635
cn3	Pdgfrb^tm1Msas/Pdgfrb^tm1Msas Tg(Nes-cre)1Nogu/?	involves: 129 * C57BL/6	MGI:3717562

Genotype
MGI:3713502

cn1

• Allelic Composition: Pdgfrb^tm1Msas/Pdgfrb^tm1Msas
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

increased apoptosis ( J:110798 )

• apoptotic cell numbers increase from 56.9% from 23.3% in wild-type when Cre-transfected dermal fibrblasts are cultured

• when cultured Cre-transfected cells are treated with H₂O₂ following treatement with 1nM human PDGFB, whereas wild-type cells are protected from hydrogen peroxide-induced cell death by 1nM PDGFB

decreased cell proliferation ( J:110798 )

• after adenoviral-Cre transfection of dermal fibroblasts, proliferation is significantly decreased in response to 1nM human PDGFB or 10% fetal bovine serum, FBS

homeostasis/metabolism

impaired wound healing ( J:110798 )

• in vitro repair of a scratch wound is inhibited in Cre-transfected dermal fibroblasts; a scratch wound in a cell monolayer remains unclosed after 48 hours in culture with Pdgfb, whereas the wound is closed in wild-type cultures and significantly reduced in wild-type cultures with 10% FBS after 48 hours; closure is almost completely suppressed after culture with 10% FBS

Genotype
MGI:5790635

cn2

• Allelic Composition: Pdgfrb^tm1Msas/Pdgfrb^tm1Msas; Tg(Nes-cre)1Nogu/0
• Genetic Background: B6J.Cg-Pdgfrb^tm1Msas Tg(Nes-cre)1Nogu

behavior/neurological

abnormal social investigation ( J:229179 )

♂ • mice exhibit social interaction deficits, showing increased non-affiliate behavior (leaving), and decreased active social contact and passive social contact compared to controls

♂ • treatment with the drug T-817MA ameliorates these social behavior deficits

nervous system

decreased neuron number ( J:229179 )

♂ • mice show reduced density of parvalbumin-immunoreactive neurons in the medical prefrontal cortex, hippocampus, amygdala, and superior colliculus

♂ • treatment with the drug T-817MA restores the number of parvalbumin+ neurons

abnormal nervous system electrophysiology ( J:229179 )

♂ • mice exhibit a reduction in auditory phase-locked gamma oscillations

♂ • treatment with the drug T-817MA restores regular phase-locked gamma oscillations

reduced sensorimotor gating ( J:229179 )

♂ • mice show deficits in sensorimotor gating

♂ • treatment with the drug T-817MA ameliorates sensorimotor gating defects

decreased prepulse inhibition ( J:229179 )

♂ • mice show deficits in prepulse inhibition

♂ • treatment with the drug T-817MA ameliorates sensorimotor gating defects

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
autism spectrum disorder		DOID:0060041		J:229179
schizophrenia		DOID:5419	OMIM:181500	J:229179

Genotype
MGI:3717562

cn3

• Allelic Composition: Pdgfrb^tm1Msas/Pdgfrb^tm1Msas; Tg(Nes-cre)1Nogu/?
• Genetic Background: involves: 129 * C57BL/6

homeostasis/metabolism

increased susceptibility to neuronal excitotoxicity ( J:110616 )

• following injection of NMDA, the volume of neuron apoptosis damage caused by excitotoxicity is increased compared to in wild-type mice

abnormal response to injury ( J:110616 )

• damage to the brain measured by TCC staining is increased following cryogenic injury

nervous system

increased susceptibility to neuronal excitotoxicity ( J:110616 )

• following injection of NMDA, the volume of neuron apoptosis damage caused by excitotoxicity is increased compared to in wild-type mice

cellular

increased susceptibility to neuronal excitotoxicity ( J:110616 )

• following injection of NMDA, the volume of neuron apoptosis damage caused by excitotoxicity is increased compared to in wild-type mice