Phenotypes associated with this allele

• Allele Symbol: Tgif1^tm1Pah
• Allele Name: targeted mutation 1, Pamela A Hoodless
• Allele ID: MGI:3652354
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Tgif1^tm1Pah/Tgif1^tm1Pah	B6.129-Tgif1^tm1Pah	MGI:3652959
hm2	Tgif1^tm1Pah/Tgif1^tm1Pah	involves: 129S/SvEv * CD-1	MGI:3652957

Genotype
MGI:3652959

hm1

• Allelic Composition: Tgif1^tm1Pah/Tgif1^tm1Pah
• Genetic Background: B6.129-Tgif1^tm1Pah

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

embryo

embryonic growth retardation ( J:109616 )

• growth retardation is detectable at E8; from embryos staged at E8-8.25, development is delayed by 2 to 3 somites in homozygotes

growth/size/body

embryonic growth retardation ( J:109616 )

• growth retardation is detectable at E8; from embryos staged at E8-8.25, development is delayed by 2 to 3 somites in homozygotes

Genotype
MGI:3652957

hm2

• Allelic Composition: Tgif1^tm1Pah/Tgif1^tm1Pah
• Genetic Background: involves: 129S/SvEv * CD-1

growth/size/body

decreased body size ( J:109616 )

• ~20% of homozygotes show growth retardation at 3 weeks of age

• Background Sensitivity: on the B6.129-Tgif^tm1Pah congenic background, no defects are seen

situs ambiguus ( J:109616 )

• 13% of homozygotes display laterality defects including situs ambiguus involving abnormal lung lobe numbers (3 incidences) or polysplenia (1 case) when examined at 3 weeks of age

• Background Sensitivity: on the B6.129-Tgif^tm1Pah congenic background, no defects are seen

situs inversus ( J:109616 )

• 13% of homozygotes display laterality defects, mainly involving heart position such as situs inversus when examined at 3 weeks of age

• Background Sensitivity: on the B6.129-Tgif^tm1Pah congenic background, no defects are seen

cellular

abnormal cell cycle ( J:109616 )

• in culture, quiescent mutant MEFs re-enter the cell cycle at a ~2-fold lower rate than wild-type cells when stimulated with serum

decreased cell proliferation ( J:109616 )

• in cultured primary MEFs, cells from homozygous mice proliferate more slowly and accumulate at a lower density than wild-type or heterozygous cells

limbs/digits/tail

kinked tail ( J:109616 )

• some homozygotes have a kinked tail

• Background Sensitivity: on the B6.129-Tgif^tm1Pah congenic background, no developmental defects are seen