Phenotypes associated with this allele

• Allele Symbol: Htr2a^tm2Grch
• Allele Name: targeted mutation 2, Jay A Gingrich
• Allele ID: MGI:3653427
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
ht1	Htr2a^tm1Grch/Htr2a^tm2Grch	129S6/SvEv-Htr2a^tm1Grch Htr2a^tm2Grch	MGI:3663440
ht2	Htr2a^tm1Grch/Htr2a^tm2Grch	involves: 129S6/SvEvTac	MGI:3663443
cn3	Emx1^tm1(cre)Ito/Emx1^+ Htr2a^tm1Grch/Htr2a^tm2Grch	129S6.Cg-Htr2a^tm1Grch Htr2a^tm2Grch Emx1^tm1(cre)Ito	MGI:3663441
cn4	Htr2a^tm1Grch/Htr2a^tm2Grch Slc6a4^tm1(cre)Grch/Slc6a4^+	involves: 129S6/SvEvTac	MGI:3663444

Genotype
MGI:3663440

ht1

• Allelic Composition: Htr2a^tm1Grch/Htr2a^tm2Grch
• Genetic Background: 129S6/SvEv-Htr2a^tm1Grch Htr2a^tm2Grch

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal anxiety-related response ( J:110958 )

• reduced inhibition in conflict anxiety paradigms without affecting fear-conditioned and depression-related behaviors

• home cage activity did not differ

• motor coordination, strength, and sensory processing were unimpaired

• baseline concentrations and the rise in corticosterone release following anxiety tests were comparable

• cell number, mantel thickness, barrel field formation, or the expression of GABA containing neuronal markers are normal

decreased anxiety-related response ( J:110958 )

• explored the lit compartment to a greater extent in the dark-light choice test

• explored the riskier portions of the elevated plus-maze to a greater extent

• exhibited a shorter latency to begin feeding in a novel environment

increased vertical activity ( J:110958 )

• in open field

Genotype
MGI:3663443

ht2

• Allelic Composition: Htr2a^tm1Grch/Htr2a^tm2Grch
• Genetic Background: involves: 129S6/SvEvTac

behavior/neurological

abnormal anxiety-related response ( J:110958 )

• reduced inhibition in conflict anxiety paradigms without affecting fear-conditioned and depression-related behaviors in the dark-light choice test

Genotype
MGI:3663441

cn3

• Allelic Composition: Emx1^tm1(cre)Ito/Emx1⁺; Htr2a^tm1Grch/Htr2a^tm2Grch
• Genetic Background: 129S6.Cg-Htr2a^tm1Grch Htr2a^tm2Grch Emx1^tm1(cre)Ito

behavior/neurological

behavior/neurological phenotype ( J:110958 )

N • selective restoration of 5HT2AR signaling to the cortex normalized conflict anxiety behaviors

N • exhibited wild-type levels of anxiety-like behavior as measured by the open field test, dark-light choice test, and novelty suppressed feeding paradigm

N • depression-related paradigms, such as the forced swim test and tail suspension test, were unchanged compared to un-restored homozygous null litter mate

Genotype
MGI:3663444

cn4

• Allelic Composition: Htr2a^tm1Grch/Htr2a^tm2Grch; Slc6a4^tm1(cre)Grch/Slc6a4⁺
• Genetic Background: involves: 129S6/SvEvTac

behavior/neurological

abnormal anxiety-related response ( J:110958 )

• reduced inhibition in conflict anxiety paradigms without affecting fear-conditioned and depression-related behaviors in the dark-light choice test

• selective restoration of 5HT2AR signaling to the thalamus did not normalized conflict anxiety behaviors