Phenotypes associated with this allele

• Allele Symbol: Gt(ROSA)26Sor^tm2(DTA)Riet
• Allele Name: targeted mutation 2, Dieter Riethmacher
• Allele ID: MGI:3653504
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Gt(ROSA)26Sor^tm2(DTA)Riet/Gt(ROSA)26Sor^tm2(DTA)Riet	involves: 129P2/OlaHsd	MGI:3653838
cn2	Gt(ROSA)26Sor^tm2(DTA)Riet/Gt(ROSA)26Sor^+ Tg(PLAT-cre)116Sdu/0	involves: 129P2/OlaHsd	MGI:4438211
cn3	Gt(ROSA)26Sor^tm2(DTA)Riet/Gt(ROSA)26Sor^+ Cnp^tm1(cre)Kan/Cnp^+	involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ	MGI:3653839
cn4	Gt(ROSA)26Sor^tm2(DTA)Riet/Gt(ROSA)26Sor^tm2(DTA)Riet Tg(Gdf9-icre)5092Coo/0	involves: 129P2/OlaHsd * C57BL/6	MGI:4430336
cn5	Gt(ROSA)26Sor^tm2(DTA)Riet/Gt(ROSA)26Sor^+ H2az2^Tg(Wnt1-cre)11Rth/H2az2^+	involves: 129P2/OlaHsd * C57BL/6J * CBA/J	MGI:4438212

Genotype
MGI:3653838

hm1

• Allelic Composition: Gt(ROSA)26Sor^tm2(DTA)Riet/Gt(ROSA)26Sor^tm2(DTA)Riet
• Genetic Background: involves: 129P2/OlaHsd

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:110983 )

• completely normal and fertile and free of any of the defects observed in homozygous Gt(ROSA)26Sor ^tm1Riet

Genotype
MGI:4438211

cn2

• Allelic Composition: Gt(ROSA)26Sor^tm2(DTA)Riet/Gt(ROSA)26Sor⁺; Tg(PLAT-cre)116Sdu/0
• Genetic Background: involves: 129P2/OlaHsd

nervous system

abnormal parasympathetic ganglion morphology ( J:157532 )

• no parasympathetic precursors are detectable at E12.5

Genotype
MGI:3653839

cn3

• Allelic Composition: Gt(ROSA)26Sor^tm2(DTA)Riet/Gt(ROSA)26Sor⁺; Cnp^tm1(cre)Kan/Cnp⁺
• Genetic Background: involves: 129P2/OlaHsd * 129S1/Sv * 129X1/SvJ

mortality/aging

postnatal lethality, complete penetrance ( J:110983 )

• double mutants die between P12 and P14

behavior/neurological

tremors ( J:110983 )

• double mutants develop tremors around P8-10

paraparesis ( J:110983 )

• double mutants develop hindlimb weakness around P8-10

growth/size/body

weight loss ( J:110983 )

• around P8-10 double mutants begin to lose weight

nervous system

absent oligodendrocytes ( J:110983 )

• from P0 to P14, no oligodendrocytes can be detected in double mutants

axon degeneration ( J:110983 )

• large clusters of unmyelinated axons that show signs of degeneration and are not surrounded by Schwann cells are seen

demyelination ( J:110983 )

• loss of oligodendrocytes and a subset of Schwann cells results in a dramatic decrease in the number of myelinated fibers in the nerves and large clusters of unmyelinated axons are seen

Genotype
MGI:4430336

cn4

• Allelic Composition: Gt(ROSA)26Sor^tm2(DTA)Riet/Gt(ROSA)26Sor^tm2(DTA)Riet; Tg(Gdf9-icre)5092Coo/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

cellular

absent oocytes ( J:157008 )

♀ • no oocytes are observed in ovaries of 8-week old females

mortality/aging

mortality/aging ( J:157008 )

N • mice are viable

reproductive system

absent oocytes ( J:157008 )

♀ • no oocytes are observed in ovaries of 8-week old females

Genotype
MGI:4438212

cn5

• Allelic Composition: Gt(ROSA)26Sor^tm2(DTA)Riet/Gt(ROSA)26Sor⁺; H2az2^{Tg(Wnt1-cre)11Rth}/H2az2⁺
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J * CBA/J

nervous system

abnormal facial nerve morphology ( J:157532 )

• numerous anomalies are evident in the branching pattern of the facial nerve