All Phenotypes Cd8b1<tm1Litt> MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Cd8b1^tm1Litt
targeted mutation 1, Dan R Littman
MGI:3654103

Summary

5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cd8b1^tm1Litt/Cd8b1^tm1Litt	involves: 129S4/SvJae * C57BL/6J	MGI:3665227
cx2	Cd8a^tm1Asin/Cd8a^tm1Asin Cd8b1^tm1Litt/Cd8b1^tm1Litt H2-Ab1^b-tm1Gru/H2-Ab1^b-tm1Gru	involves: 129 * FVB/N	MGI:3760624
cx3	Cd8b1^tm1Litt/Cd8b1⁺ Tg(Cd8b1)46Erob/0	involves: 129S4/SvJae * C57BL/6 * CBA	MGI:3665228
cx4	Cd8b1^tm1Litt/Cd8b1⁺ Tg(TcraH-Y,TcrbH-Y)71Vbo/0	involves: 129S4/SvJae * C57BL/6J * DBA/2	MGI:3665230
cx5	Cd8b1^tm1Litt/Cd8b1^tm1Litt Tg(TcraH-Y,TcrbH-Y)71Vbo/0	involves: 129S4/SvJae * C57BL/6J * DBA/2	MGI:3665231

Allelic Composition	Cd8b1^tm1Litt/Cd8b1^tm1Litt
Genetic Background	involves: 129S4/SvJae * C57BL/6J

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8b1^tm1Litt mutation (1 available); any Cd8b1 mutation (20 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased thymocyte number ( J:110916 )

• mice have 5-fold fewer CD8-lineage thymocytes compared to wild-type; there is a lack of mature CD8-lineage T cells

abnormal T cell subpopulation ratio ( J:110916 )

• CD8 to CD4 ratio is 6.6 fold lower than in wild-type

decreased CD8-positive, alpha-beta T cell number ( J:110916 , J:112551 )

• there is a lack of mature CD8-lineage T cells (J:110916)

• number of mature single positive CD8+ thymocytes is reduced 5-fold vs wild-type (J:112551)

• numbers in peripheral lymph nodes are reduced 5-fold also (J:112551)

abnormal cytotoxic T cell physiology ( J:112551 )

• splenocytes from mutants show a normal cytotoxic T lymphocyte response comparable to heterozygous mice to allogeneic stimuli even though mutants have only 20% of normal numbers of CD8+ T cells

homeostasis/metabolism

abnormal enzyme/coenzyme activity ( J:110916 )

• thymocytes display 6-fold less CD8-associated Lck activity vs wild-type

immune system

decreased thymocyte number ( J:110916 )

• mice have 5-fold fewer CD8-lineage thymocytes compared to wild-type; there is a lack of mature CD8-lineage T cells

abnormal T cell subpopulation ratio ( J:110916 )

• CD8 to CD4 ratio is 6.6 fold lower than in wild-type

decreased CD8-positive, alpha-beta T cell number ( J:110916 , J:112551 )

• there is a lack of mature CD8-lineage T cells (J:110916)

• number of mature single positive CD8+ thymocytes is reduced 5-fold vs wild-type (J:112551)

• numbers in peripheral lymph nodes are reduced 5-fold also (J:112551)

abnormal cytotoxic T cell physiology ( J:112551 )

• splenocytes from mutants show a normal cytotoxic T lymphocyte response comparable to heterozygous mice to allogeneic stimuli even though mutants have only 20% of normal numbers of CD8+ T cells

endocrine/exocrine glands

decreased thymocyte number ( J:110916 )

• mice have 5-fold fewer CD8-lineage thymocytes compared to wild-type; there is a lack of mature CD8-lineage T cells

Allelic Composition	Cd8a^tm1Asin/Cd8a^tm1Asin Cd8b1^tm1Litt/Cd8b1^tm1Litt H2-Ab1^b-tm1Gru/H2-Ab1^b-tm1Gru
Genetic Background	involves: 129 * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8a^tm1Asin mutation (0 available); any Cd8a mutation (36 available)
Cd8b1^tm1Litt mutation (1 available); any Cd8b1 mutation (20 available)
H2-Ab1^b-tm1Gru mutation (11 available); any H2-Ab1 mutation (82 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

decreased CD4-positive, alpha-beta T cell number ( J:126026 )

• these cells are almost absent in the thymus and the lymph nodes

increased CD8-positive, alpha-beta T cell number ( J:126026 )

• 1.6 fold increase in number of these cells in the lymph nodes, compared to mice that have a wild-type allele for Cd8a on this mutant background

hematopoietic system

decreased CD4-positive, alpha-beta T cell number ( J:126026 )

• these cells are almost absent in the thymus and the lymph nodes

increased CD8-positive, alpha-beta T cell number ( J:126026 )

• 1.6 fold increase in number of these cells in the lymph nodes, compared to mice that have a wild-type allele for Cd8a on this mutant background

Allelic Composition	Cd8b1^tm1Litt/Cd8b1⁺ Tg(Cd8b1)46Erob/0
Genetic Background	involves: 129S4/SvJae * C57BL/6 * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8b1^tm1Litt mutation (1 available); any Cd8b1 mutation (20 available)
Tg(Cd8b1)46Erob mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal T cell subpopulation ratio ( J:110916 )

• CD8 to CD4 ratio is 2.2-fold lower than in wild-type

decreased T cell number ( J:110916 )

• there is a lack of mature CD8-lineage T cells

decreased thymocyte number ( J:110916 )

• mice have 3-fold fewer CD8-lineage thymocytes compared to wild-type

homeostasis/metabolism

abnormal enzyme/coenzyme activity ( J:110916 )

• thymocytes display 4-fold less CD8-associated Lck activity vs wild-type

• activity of Lck is reduced but significant levels are detected in mutants; there is a slight reduction in CD8a-associated activity compared to wild-type

hematopoietic system

abnormal T cell subpopulation ratio ( J:110916 )

• CD8 to CD4 ratio is 2.2-fold lower than in wild-type

decreased T cell number ( J:110916 )

• there is a lack of mature CD8-lineage T cells

decreased thymocyte number ( J:110916 )

• mice have 3-fold fewer CD8-lineage thymocytes compared to wild-type

endocrine/exocrine glands

decreased thymocyte number ( J:110916 )

• mice have 3-fold fewer CD8-lineage thymocytes compared to wild-type

Allelic Composition	Cd8b1^tm1Litt/Cd8b1⁺ Tg(TcraH-Y,TcrbH-Y)71Vbo/0
Genetic Background	involves: 129S4/SvJae * C57BL/6J * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8b1^tm1Litt mutation (1 available); any Cd8b1 mutation (20 available)
Tg(TcraH-Y,TcrbH-Y)71Vbo mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

decreased double-positive T cell number ( J:112551 )

• male transgenic mice show disruption of relative and absolute numbers of double positive T cells in the thymus

immune system

decreased double-positive T cell number ( J:112551 )

• male transgenic mice show disruption of relative and absolute numbers of double positive T cells in the thymus

Allelic Composition	Cd8b1^tm1Litt/Cd8b1^tm1Litt Tg(TcraH-Y,TcrbH-Y)71Vbo/0
Genetic Background	involves: 129S4/SvJae * C57BL/6J * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8b1^tm1Litt mutation (1 available); any Cd8b1 mutation (20 available)
Tg(TcraH-Y,TcrbH-Y)71Vbo mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal negative T cell selection ( J:112551 )

• mice show significant recovery of double positive cell numbers, indicating that negative selection is impaired

abnormal positive T cell selection ( J:112551 )

• thymocytes in female transgenic mice show a 20-fold reduction in CD4-CD8+ T cells compared to CD8b1^tm1Litt heterozygous transgenic mice showing defective positive selection

increased CD4-positive, alpha-beta T cell number ( J:112551 )

• there is an increase in mature CD4+ T cells in thymus

decreased CD8-positive, alpha-beta T cell number ( J:112551 )

• in peripheral lymphoid organs, a 7-fold reduction is observed

abnormal lymph node cell ratio ( J:112551 )

• lymph nodes in male mice have 50% higher CD3+ lymphocytes compared to heterozygous transgenic males

hematopoietic system

abnormal negative T cell selection ( J:112551 )

• mice show significant recovery of double positive cell numbers, indicating that negative selection is impaired

abnormal positive T cell selection ( J:112551 )

• thymocytes in female transgenic mice show a 20-fold reduction in CD4-CD8+ T cells compared to CD8b1^tm1Litt heterozygous transgenic mice showing defective positive selection

increased CD4-positive, alpha-beta T cell number ( J:112551 )

• there is an increase in mature CD4+ T cells in thymus

decreased CD8-positive, alpha-beta T cell number ( J:112551 )

• in peripheral lymphoid organs, a 7-fold reduction is observed

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