Phenotypes associated with this allele

• Allele Symbol: Tg(KRT14-Vegfa)3Dtm
• Allele Name: transgene insertion 3, Michael Detmar
• Allele ID: MGI:3655101
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(KRT14-Vegfa)3Dtm/0	FVB/N-Tg(KRT14-Vegfa)3Dtm	MGI:3655625

Genotype
MGI:3655625

tg1

• Allelic Composition: Tg(KRT14-Vegfa)3Dtm/0
• Genetic Background: FVB/N-Tg(KRT14-Vegfa)3Dtm

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

skin edema ( J:92643 )

• pronounced in dermis after oxazolone challenge

delayed wound healing ( J:111924 )

• despite increased angiogenesis and vasculogenesis, wound closure is slightly delayed in transgenic mice compared to wild-type

immune system

abnormal leukocyte adhesion ( J:104245 )

• mice display enhanced density of adherent leukocytes (251.9/mm² vs 61.3/mm² in wild-type)

enhanced leukocyte tethering or rolling ( J:104245 )

• postcapillary venules show an increased proportion of rolling leukocytes compared to venules in wild-type skin (47.9% vs 15.6%, respectively)

increased mast cell number ( J:104245 )

• there is increased mast cell localization to sites of angiogenesis compared to controls (35% increase in mast cell density compared to wild-type)

increased macrophage cell number ( J:111924 )

• number of macrophages in granulation tissue is increased 2-fold compared to wild-type at day 7 after wounding but at days 10 and 14, numbers are equivalent to wild-type

enlarged lymph nodes ( J:92643 )

• 24 hours after oxazolone challenge, lymph nodes draining the challenges side of transgenics are enlarged

abnormal lymphatic vessel morphology ( J:111924 )

• transgenic mice rapidly form a lymphatic vasculature at the site of the wound whereas no lymphatic vessels are found at day 7 post-wounding and only a few sprouting vessels are seen at day 10 in wild-type mice

enlarged lymphatic vessel ( J:92643 )

• mutants show enlarged dermal lymphatics in non-inflamed skin compared to wild-type

• lymphatic vessels are significantly larger in mutants (935 um² compared to wild-type, 592 um²) 30 days after DTH induction

• after induction of delayed-type hypersensitivity (DTH) reactions, skin of wild-type mice show tissue edema and a transient enlargement of lymphatic vessels which declines after 7 days and returns to baseline in 1 month; mutants show prolonged ear-swelling, and enhanced and persistent lymphatic vessel enlargement still detectable 1 month after initiation

hematopoietic system

abnormal leukocyte adhesion ( J:104245 )

• mice display enhanced density of adherent leukocytes (251.9/mm² vs 61.3/mm² in wild-type)

enhanced leukocyte tethering or rolling ( J:104245 )

• postcapillary venules show an increased proportion of rolling leukocytes compared to venules in wild-type skin (47.9% vs 15.6%, respectively)

increased mast cell number ( J:104245 )

• there is increased mast cell localization to sites of angiogenesis compared to controls (35% increase in mast cell density compared to wild-type)

increased macrophage cell number ( J:111924 )

• number of macrophages in granulation tissue is increased 2-fold compared to wild-type at day 7 after wounding but at days 10 and 14, numbers are equivalent to wild-type

cardiovascular system

abnormal capillary morphology ( J:104245 )

• in 3-week old transgenics, capillaries in the skin show a highly increased tortuosity index (+69%) compared to wild-type with a corresponding average decreased length without branching

abnormal capillary branching pattern ( J:104245 )

increased capillary tortuosity ( J:104245 )

• at 3 weeks of age

abnormal angiogenesis ( J:92643 , J:104245 , J:111924 )

• mutants have significantly higher numbers of blood vessels in normal skin compared to wild-type (J:92643)

• 3-week old transgenic mice show increased angiogenesis in the skin (J:104245)

• transgenic mice display an increased density of blood microvessels in the dermis of the abdomen, dorsal region and ear at 3 days of age compared to controls; increase is less dramatic at 11 days, 3 weeks and 6 weeks (J:104245)

• vascularization of granulation tissue remains elevated for three weeks following wounding, compared to control mice (J:111924)

increased vascular permeability ( J:92643 , J:104245 )

• extravasation of dye in non-inflamed ears of transgenic mice is significantly higher (34 ng/mg) than wild-type (7 ng/mg) (J:92643)

• 24 hours after oxazolone challenge, baseline leakage increased 10 fold in wild-type and 11.5-fold in transgenic animals compared to vehicle-treated mice; 7 days after treatment leakage is diminished in wild-type and mutant mice but is still much higher in mutants (J:92643)

• induced new dermal capillaries in the ear skin are hyperpermeable (J:104245)

integument

skin edema ( J:92643 )

• pronounced in dermis after oxazolone challenge

mixed cellular infiltration to dermis ( J:92643 )

• dense inflammatory infiltrates of CD4+ lymphocytes within the dermis, as well as CD8+ lymphocytes within the epidermis

abnormal epidermal layer morphology ( J:92643 )

• epidermis contains fingerlike, anatomosing extensions protruding into the dermis after oxazolone challenge

parakeratosis ( J:92643 )

• epidermis of the ears is parakeratotic 1 month after oxazolone challenge

epidermal hyperplasia ( J:92643 )

• 1 month after oxazolone challenge, ears display epidermal hyperplasia

psoriasis ( J:92643 )

• 1 month after oxazolone challenge, ears of mice show a psoriasis-like phenotype

cellular

abnormal leukocyte adhesion ( J:104245 )

• mice display enhanced density of adherent leukocytes (251.9/mm² vs 61.3/mm² in wild-type)

enhanced leukocyte tethering or rolling ( J:104245 )

• postcapillary venules show an increased proportion of rolling leukocytes compared to venules in wild-type skin (47.9% vs 15.6%, respectively)

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
psoriasis		DOID:8893	OMIM:PS177900	J:92643