Phenotypes associated with this allele

• Allele Symbol: Tg(Pcp2-cre)1Amc
• Allele Name: transgene insertion 1, Andrew P McMahon
• Allele ID: MGI:3656031
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Shh^tm2Amc/Shh^tm3Amc Tg(Pcp2-cre)1Amc/0	involves: 129S1/Sv * 129X1/SvJ * Black Swiss	MGI:3656218
cn2	Cdk5r1^tm2Ohsh/Cdk5r1^tm2Ohsh Tg(Pcp2-cre)1Amc/0	involves: C57BL/6 * C57BL/6N	MGI:5824372

Genotype
MGI:3656218

cn1

• Allelic Composition: Shh^tm2Amc/Shh^tm3Amc; Tg(Pcp2-cre)1Amc/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * Black Swiss

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal motor capabilities/coordination/movement ( J:92189 )

• pups display movement disorders characteristic of abnormal cerebellar function

ataxia ( J:92189 )

nervous system

abnormal cerebellum morphology ( J:92189 )

• at E18.5, cerebellum is detected to be abnormal with immature fissures and a reduction in the external granule layer; however no difference in cerebellar volume is evident between mutants and wild-type

abnormal cerebellum development ( J:92189 )

abnormal cerebellar lobule formation ( J:92189 )

• at E18.5 mutants have 5 rudimentary lobules

• at P5, cerebellum has small primitive lobules

thin external granule cell layer ( J:92189 )

abnormal cerebellar Purkinje cell layer ( J:92189 )

• at P5 cells are dispersed in a thinner layer than in control Shh^tm2Amc/Shh^tm3Amc control mice or Shh^tm2Amc/Shh^tm3Amc;Tg(Pax2-cre)1Amc mice

• dendrite projections of cells are hard to distinguish

• at P14, there is a layer of Purkinje neurons that is better organized than in Shh^tm2Amc/Shh^tm3Amc;Tg(Pax2-cre)1Amc mice

decreased Purkinje cell number ( J:92189 )

• at P5 number is reduced by 20% compared to controls

• in adults, a 35% reduction is apparent

small cerebellum ( J:92189 )

• in adults, cerebellum is small but has distinct lobes with Purkinje cells organized into a 1-2 cell layer overlayed by a thin inner germinal layer

Genotype
MGI:5824372

cn2

• Allelic Composition: Cdk5r1^tm2Ohsh/Cdk5r1^tm2Ohsh; Tg(Pcp2-cre)1Amc/0
• Genetic Background: involves: C57BL/6 * C57BL/6N

behavior/neurological

impaired coordination ( J:215344 )

• mice show a significantly impaired ability to maintain balance on a rotating rod at 3 different speeds (16, 24 and 32 rpm)

• in a balance beam test, mice show a significantly increased number of paw slips at two different beam diameters (11 mm and 28 mm)

• however, no gait disturbances are detected in a footprint test

nervous system

nervous system phenotype ( J:215344 )

N • cerebellar histology is grossly normal, with no significant alterations in Purkinje cell morphology in terms of alignment, density, axonal elongation or dendritic structure

impaired synaptic plasticity ( J:215344 )

• mice show impaired cerebellar long-term synaptic plasticity (LTD and LTP) at the parallel fiber (PF)-Purkinje cell synapse

reduced long-term potentiation ( J:215344 )

• although Purkinje cells exhibit long-lasting potentiated PF-evoked excitatory post synaptic currents (EPSCs) after 300 PF-stimuli at 1 Hz, the amplitude of LTP is significantly smaller than that in control mice

absent long-term depression ( J:215344 )

• Purkinje cells fail to exhibit depressed PF-evoked EPSCs after conjunctive stimuli, unlike in control mice