Phenotypes associated with this allele

• Allele Symbol: Tg(H2-K-Cd86)7All
• Allele Name: transgene insertion 7, James P Allison
• Allele ID: MGI:3656399
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tcrb^tm1Mom/Tcrb^tm1Mom Tg(H2-K-Cd86)7All/0	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3662635
cx2	Cd28^tm1Mak/Cd28^tm1Mak Tg(H2-K-Cd86)7All/0	involves: BALB/c * C57BL/6 * CBA	MGI:3662672
tg3	Tg(H2-K-Cd86)7All/0	B6.Cg-Tg(H2-K-Cd86)7All	MGI:3662662
tg4	Tg(H2-K-Cd86)7All/0	involves: BALB/c * C57BL/6 * CBA	MGI:3662665
tg5	Tg(H2-K-Cd86)7All/0	involves: C57BL/6J * CBA	MGI:3662634

Genotype
MGI:3662635

cx1

• Allelic Composition: Tcrb^tm1Mom/Tcrb^tm1Mom; Tg(H2-K-Cd86)7All/0
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

hematopoietic system

hematopoietic system phenotype ( J:112475 )

N • mice have same numbers of B cells in spleen and lymph nodes as control littermates

abnormal B cell morphology ( J:112475 )

• transgenic B cells increase in size and show upregulated MHC class II when mice are injected with T cells

abnormal B cell differentiation ( J:112475 )

• mice show a substantial decrease in B220+ cells in bone marrow when mice receive nontransgenic T cells

decreased B cell number ( J:112475 )

• when syngeneic wild-type T cells are transferred into Tcrb-deficient transgenic hosts, after 3 weeks the number of B cells in peripheral lymphoid tissues is decreased by ~50% compared with Tcrb-deficient mice not injected with T cells

immune system

abnormal B cell morphology ( J:112475 )

• transgenic B cells increase in size and show upregulated MHC class II when mice are injected with T cells

abnormal B cell differentiation ( J:112475 )

• mice show a substantial decrease in B220+ cells in bone marrow when mice receive nontransgenic T cells

decreased B cell number ( J:112475 )

• when syngeneic wild-type T cells are transferred into Tcrb-deficient transgenic hosts, after 3 weeks the number of B cells in peripheral lymphoid tissues is decreased by ~50% compared with Tcrb-deficient mice not injected with T cells

Genotype
MGI:3662672

cx2

• Allelic Composition: Cd28^tm1Mak/Cd28^tm1Mak; Tg(H2-K-Cd86)7All/0
• Genetic Background: involves: BALB/c * C57BL/6 * CBA

hematopoietic system

abnormal T cell differentiation ( J:112266 )

• compared to wild-type Cd28 transgenic mice Cd28-deficient mice show no lymphocyte expansion or increase in CD8+ T cell population

immune system

abnormal T cell differentiation ( J:112266 )

• compared to wild-type Cd28 transgenic mice Cd28-deficient mice show no lymphocyte expansion or increase in CD8+ T cell population

Genotype
MGI:3662662

tg3

• Allelic Composition: Tg(H2-K-Cd86)7All/0
• Genetic Background: B6.Cg-Tg(H2-K-Cd86)7All

immune system

decreased B cell number ( J:112266 )

• there is a decreased proportion of B cells in the spleens compared to controls

decreased CD4-positive, alpha-beta T cell number ( J:112266 )

• a similarly small but significant decrease in percentage of single positive CD4 thymocytes is observed

• in the spleen there is a lower percentage of CD4+ T cells that is more evident than in the thymus

increased T cell number ( J:112266 )

• in the spleens there is an increase in T cell number

increased CD8-positive, alpha-beta T cell number ( J:112266 )

• a small but significant increase in percentage of single-positive CD8 thymocytes is seen compared to controls

• in the spleen there is a higher percentage of CD8+ T cells that is more evident than in the thymus

lymph node hyperplasia ( J:112266 )

• in peripheral lymph nodes, there is a significant lymphocyte expansion with lymph node cells increased >6-fold over littermate controls

• Background Sensitivity: on a B6 congenic background peripheral lymphocyte proliferation is lower compared to that on a (BALB/c x C57BL/6)F1 background

lymphoid hyperplasia ( J:112266 )

• expression of the Cd86 transgene results in hyperplasia of peripheral lymphoid organs

abnormal inflammatory response ( J:112266 )

• there is extensive lymphoid infiltration of other organs including the lungs

hematopoietic system

decreased B cell number ( J:112266 )

• there is a decreased proportion of B cells in the spleens compared to controls

decreased CD4-positive, alpha-beta T cell number ( J:112266 )

• a similarly small but significant decrease in percentage of single positive CD4 thymocytes is observed

• in the spleen there is a lower percentage of CD4+ T cells that is more evident than in the thymus

increased T cell number ( J:112266 )

• in the spleens there is an increase in T cell number

increased CD8-positive, alpha-beta T cell number ( J:112266 )

• a small but significant increase in percentage of single-positive CD8 thymocytes is seen compared to controls

• in the spleen there is a higher percentage of CD8+ T cells that is more evident than in the thymus

Genotype
MGI:3662665

tg4

• Allelic Composition: Tg(H2-K-Cd86)7All/0
• Genetic Background: involves: BALB/c * C57BL/6 * CBA

hematopoietic system

spleen hyperplasia ( J:112266 )

• number of splenocytes is increased by >200-fold

• on a (BALB/c x C57BL/6)F1 background peripheral lymphocyte proliferation is greater with splenocyte numbers compared to a coisogenic B6 background

increased splenocyte number ( J:112266 )

• number of splenocytes is increased by >200-fold

immune system

spleen hyperplasia ( J:112266 )

• number of splenocytes is increased by >200-fold

• on a (BALB/c x C57BL/6)F1 background peripheral lymphocyte proliferation is greater with splenocyte numbers compared to a coisogenic B6 background

increased splenocyte number ( J:112266 )

• number of splenocytes is increased by >200-fold

lymph node hyperplasia ( J:112266 )

• in peripheral lymph nodes, there is a significant lymphocyte expansion with lymph node cells increased >10-fold over littermate controls

• Background Sensitivity: on a (BALB/c x C57BL/6)F1 background peripheral lymphocyte proliferation is greater compared to a coisogenic B6 background

growth/size/body

spleen hyperplasia ( J:112266 )

• number of splenocytes is increased by >200-fold

• on a (BALB/c x C57BL/6)F1 background peripheral lymphocyte proliferation is greater with splenocyte numbers compared to a coisogenic B6 background

Genotype
MGI:3662634

tg5

• Allelic Composition: Tg(H2-K-Cd86)7All/0
• Genetic Background: involves: C57BL/6J * CBA

hematopoietic system

abnormal B cell differentiation ( J:112475 )

• proportion of pre/pro B cells and immature/mature B cells is low in bone marrow in transgenic mice

• B cells are slightly larger than wild-type

decreased B cell number ( J:112475 )

• some mice expressing transgene at high levels show substantial reduction in B lymphocytes in all peripheral tissues; some mice exhibit a severe reduction in number in spleen with an increase in number the lymph nodes

increased CD4-positive, alpha-beta T cell number ( J:112475 )

• numbers of CD4+ T cells are increased in the spleen but not in lymph nodes of mice; an increased fraction of and T cells are CD44^hi

increased CD8-positive, alpha-beta T cell number ( J:112475 )

• numbers of CD8+ T cells are increased in the spleen but not in lymph nodes of mice; an increased fraction of CD8+ cells are CD44^hi

immune system

abnormal B cell differentiation ( J:112475 )

• proportion of pre/pro B cells and immature/mature B cells is low in bone marrow in transgenic mice

• B cells are slightly larger than wild-type

decreased B cell number ( J:112475 )

• some mice expressing transgene at high levels show substantial reduction in B lymphocytes in all peripheral tissues; some mice exhibit a severe reduction in number in spleen with an increase in number the lymph nodes

increased CD4-positive, alpha-beta T cell number ( J:112475 )

• numbers of CD4+ T cells are increased in the spleen but not in lymph nodes of mice; an increased fraction of and T cells are CD44^hi

increased CD8-positive, alpha-beta T cell number ( J:112475 )

• numbers of CD8+ T cells are increased in the spleen but not in lymph nodes of mice; an increased fraction of CD8+ cells are CD44^hi