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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Igktm1Rsky
targeted mutation 1, Klaus Rajewsky
MGI:3662618
Summary 6 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
 		Ightm2Rsky/Igh+
Igktm1Rsky/Igk+ 		either: (involves: 129P2/OlaHsd) or (involves: 129P2/OlaHsd * BALB/c) MGI:3689731
cx2
 		Ightm2Cgn/Igh+
Igktm1Rsky/Igk+ 		either: (involves: 129P2/OlaHsd) or (involves: 129P2/OlaHsd * BALB/c) MGI:3689733
cx3
 		Blnktm1Kplm/Blnktm1Kplm
Ightm4Cgn/Igh+
Igktm1Rsky/Igk+ 		involves: 129P2/OlaHsd MGI:3844072
cx4
 		Ightm2Cgn/Ightm2Cgn
Igktm1Rsky/Igktm1Rsky
Trp53tm1Tyj/Trp53tm1Tyj
Xrcc4tm1Fwa/Xrcc4tm1Fwa 		involves: 129P2/OlaHsd * 129S2/SvPas * 129S6/SvEvTac MGI:4437907
cx5
 		Ightm2Cgn/Ightm2Cgn
Igktm1Rsky/Igktm1Rsky
Lig4tm1Fwa/Lig4tm1Fwa
Trp53tm1Tyj/Trp53tm1Tyj 		involves: 129P2/OlaHsd * 129S2/SvPas * 129S6/SvEvTac MGI:4437910
cx6
 		Igktm1Rsky/Igk+
Igll1tm1Cgn/Igll1tm1Cgn 		involves: 129P2/OlaHsd * 129S2/SvPas * BALB/c * C57BL/6 MGI:3689735


Genotype
MGI:3689731
cx1
Allelic
Composition		 Ightm2Rsky/Igh+
Igktm1Rsky/Igk+
Genetic
Background		 either: (involves: 129P2/OlaHsd) or (involves: 129P2/OlaHsd * BALB/c)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ightm2Rsky mutation (2 available); any Igh mutation (44 available)
Igktm1Rsky mutation (1 available); any Igk mutation (26 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell differentiation ( J:111431 )
• lack 3-83Ig+ mature B cells, although on an H2d background some 3-83Ig+ immature B cells are detected in the bone marrow
• about and 8-fold and 15-fold increase in the frequency and absolute number of lambda expressing splenic B cells, respectively, compared to wild-type mice
• however, total B cell numbers are similar to wild-type

hematopoietic system
abnormal B cell differentiation ( J:111431 )
• lack 3-83Ig+ mature B cells, although on an H2d background some 3-83Ig+ immature B cells are detected in the bone marrow
• about and 8-fold and 15-fold increase in the frequency and absolute number of lambda expressing splenic B cells, respectively, compared to wild-type mice
• however, total B cell numbers are similar to wild-type




Genotype
MGI:3689733
cx2
Allelic
Composition		 Ightm2Cgn/Igh+
Igktm1Rsky/Igk+
Genetic
Background		 either: (involves: 129P2/OlaHsd) or (involves: 129P2/OlaHsd * BALB/c)
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ightm2Cgn mutation (1 available); any Igh mutation (44 available)
Igktm1Rsky mutation (1 available); any Igk mutation (26 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell differentiation ( J:111431 )
• about 90% of B cells are positive for the B1-8 heavy chain
absent pre-B cells ( J:111431 )
• the CD43- sIgM- pre B cell compartment is virtually absent

hematopoietic system
abnormal B cell differentiation ( J:111431 )
• about 90% of B cells are positive for the B1-8 heavy chain
absent pre-B cells ( J:111431 )
• the CD43- sIgM- pre B cell compartment is virtually absent




Genotype
MGI:3844072
cx3
Allelic
Composition		 Blnktm1Kplm/Blnktm1Kplm
Ightm4Cgn/Igh+
Igktm1Rsky/Igk+
Genetic
Background		 involves: 129P2/OlaHsd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Blnktm1Kplm mutation (0 available); any Blnk mutation (78 available)
Ightm4Cgn mutation (0 available); any Igh mutation (44 available)
Igktm1Rsky mutation (1 available); any Igk mutation (26 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell differentiation ( J:120460 )
• innocuous transgenic BCR on Blnk null B cells leads to enhanced development of these B cells compared to Blnk homozygotes
• two populations are detected in the bone marrow consisting of IgMlo and IgMhi groups
• both populations appear to be immature B cells as they have low surface level expression of MHC-II and CD43, and high levels of CD24
• based on intensity of these surface markers, the IgMlo population is the less mature of the two
• in vitro maturation of bone marrow cells demonstrate delayed development of these IgM+ cells
• B cells in the periphery are primarily T1 stage B cells that have a short half-life
increased transitional stage B cell number ( J:120460 )
• B cells in the periphery are primarily T1 stage B cells
decreased B cell number ( J:120460 )
• B cell numbers are decreased 2- to 3- fold in the spleen
• the B cells present are primarily T1 stage B cells
increased pre-B cell number ( J:120460 )
• pre-B cell numbers are 2-3 fold higher in the bone marrow than in transgenic controls

hematopoietic system
abnormal B cell differentiation ( J:120460 )
• innocuous transgenic BCR on Blnk null B cells leads to enhanced development of these B cells compared to Blnk homozygotes
• two populations are detected in the bone marrow consisting of IgMlo and IgMhi groups
• both populations appear to be immature B cells as they have low surface level expression of MHC-II and CD43, and high levels of CD24
• based on intensity of these surface markers, the IgMlo population is the less mature of the two
• in vitro maturation of bone marrow cells demonstrate delayed development of these IgM+ cells
• B cells in the periphery are primarily T1 stage B cells that have a short half-life
increased transitional stage B cell number ( J:120460 )
• B cells in the periphery are primarily T1 stage B cells
decreased B cell number ( J:120460 )
• B cell numbers are decreased 2- to 3- fold in the spleen
• the B cells present are primarily T1 stage B cells
increased pre-B cell number ( J:120460 )
• pre-B cell numbers are 2-3 fold higher in the bone marrow than in transgenic controls




Genotype
MGI:4437907
cx4
Allelic
Composition		 Ightm2Cgn/Ightm2Cgn
Igktm1Rsky/Igktm1Rsky
Trp53tm1Tyj/Trp53tm1Tyj
Xrcc4tm1Fwa/Xrcc4tm1Fwa
Genetic
Background		 involves: 129P2/OlaHsd * 129S2/SvPas * 129S6/SvEvTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ightm2Cgn mutation (1 available); any Igh mutation (44 available)
Igktm1Rsky mutation (1 available); any Igk mutation (26 available)
Trp53tm1Tyj mutation (12 available); any Trp53 mutation (240 available)
Xrcc4tm1Fwa mutation (0 available); any Xrcc4 mutation (26 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal class switch recombination ( J:126758 )
• class switching to IgG1 is 20% to 50% of wild-type
• class switching to IgG3 is 50% of wild-type
• IgH class switching is reduced compared to in wild-type B cells

cellular
spontaneous chromosome breakage ( J:126758 )
• B cell stimulated with anti-CD40 and IL4 exhibit metaphase chromosome breaks unlike similarly treated wild-type cells

hematopoietic system
abnormal class switch recombination ( J:126758 )
• class switching to IgG1 is 20% to 50% of wild-type
• class switching to IgG3 is 50% of wild-type
• IgH class switching is reduced compared to in wild-type B cells




Genotype
MGI:4437910
cx5
Allelic
Composition		 Ightm2Cgn/Ightm2Cgn
Igktm1Rsky/Igktm1Rsky
Lig4tm1Fwa/Lig4tm1Fwa
Trp53tm1Tyj/Trp53tm1Tyj
Genetic
Background		 involves: 129P2/OlaHsd * 129S2/SvPas * 129S6/SvEvTac
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ightm2Cgn mutation (1 available); any Igh mutation (44 available)
Igktm1Rsky mutation (1 available); any Igk mutation (26 available)
Lig4tm1Fwa mutation (2 available); any Lig4 mutation (46 available)
Trp53tm1Tyj mutation (12 available); any Trp53 mutation (240 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
abnormal class switch recombination ( J:126758 )
• class switching to IgG1 in B cells stimulated with anti-CD40 plus IL4 are 50% of wild-type

immune system
abnormal class switch recombination ( J:126758 )
• class switching to IgG1 in B cells stimulated with anti-CD40 plus IL4 are 50% of wild-type




Genotype
MGI:3689735
cx6
Allelic
Composition		 Igktm1Rsky/Igk+
Igll1tm1Cgn/Igll1tm1Cgn
Genetic
Background		 involves: 129P2/OlaHsd * 129S2/SvPas * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Igktm1Rsky mutation (1 available); any Igk mutation (26 available)
Igll1tm1Cgn mutation (1 available); any Igll1 mutation (16 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal B cell differentiation ( J:35357 )
• only 5.5% of CD43+ B cell progenitors express the kappa chain in the cytoplasm compared to 22% of wild-type cells at a similar developmental stage which express a kappa chain
decreased immature B cell number ( J:35357 )
• about a 5-fold reduction in immature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus
decreased B cell number ( J:35357 )
• about a 5-fold reduction in mature and immature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus
• in the spleen at 6 - 12 weeks of age, B cell numbers are about 1/3 that of wild-type mice
decreased mature B cell number ( J:35357 )
• about a 5-fold reduction in mature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus
decreased pre-B cell number ( J:35357 )
• about a 3-fold reduction compared to wild-type mice

hematopoietic system
abnormal B cell differentiation ( J:35357 )
• only 5.5% of CD43+ B cell progenitors express the kappa chain in the cytoplasm compared to 22% of wild-type cells at a similar developmental stage which express a kappa chain
decreased immature B cell number ( J:35357 )
• about a 5-fold reduction in immature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus
decreased B cell number ( J:35357 )
• about a 5-fold reduction in mature and immature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus
• in the spleen at 6 - 12 weeks of age, B cell numbers are about 1/3 that of wild-type mice
decreased mature B cell number ( J:35357 )
• about a 5-fold reduction in mature B cell numbers compared to wild-type mice, but a 3-fold increase compared to Igll1 mutant mice wild-type at the Igk locus
decreased pre-B cell number ( J:35357 )
• about a 3-fold reduction compared to wild-type mice




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory