Phenotypes associated with this allele

• Allele Symbol: Sell^tm1Pest
• Allele Name: targeted mutation 1, Pila Estess
• Allele ID: MGI:3663167
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sell^tm1Pest/Sell^tm1Pest	involves: 129S4/SvJae	MGI:3664422
hm2	Sell^tm1Pest/Sell^tm1Pest	involves: 129S4/SvJae * C57BL/6	MGI:3664423
ht3	Sell^tm1Pest/Sell^+	involves: 129S4/SvJae * C57BL/6	MGI:3664424

Genotype
MGI:3664422

hm1

• Allelic Composition: Sell^tm1Pest/Sell^tm1Pest
• Genetic Background: involves: 129S4/SvJae

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal leukocyte adhesion ( J:111233 )

• in untreated Sell-deficient mice, WBCs do not experience activation-dependent LFA-1 mediated arrest and adhere to high endothelial venules (HEV)

• infusion of activated platelets restores rolling capability in Sell-deficient WBCs and greater than 50% become stably adherent to HEV

impaired leukocyte tethering or rolling ( J:111233 )

• fraction of rolling Sell-deficient lymphocytes (12.1%) is reduced compared to wild-type littermates (72.1%)

• injection of activated, but not resting, human platelets enhanced rolling fraction to 48.9%, approaching a similar level to wild-type

increased T cell proliferation ( J:111233 )

• lymph node T cells from sensitized platelet-treated mutant mice proliferate comparably to wild-type in response to antigen while T cells from untreated sensitized mutants do not proliferate at all

abnormal hypersensitivity reaction ( J:111233 )

• when mutant mice are sensitized to the antigen DFNB by topical application to abdominal skin and injected with activated human platelets, a dramatic contact hypersensitivity response is elicited 5 days later, compared to no response in sham-treated sensitized mutants

hematopoietic system

abnormal leukocyte adhesion ( J:111233 )

• in untreated Sell-deficient mice, WBCs do not experience activation-dependent LFA-1 mediated arrest and adhere to high endothelial venules (HEV)

• infusion of activated platelets restores rolling capability in Sell-deficient WBCs and greater than 50% become stably adherent to HEV

impaired leukocyte tethering or rolling ( J:111233 )

• fraction of rolling Sell-deficient lymphocytes (12.1%) is reduced compared to wild-type littermates (72.1%)

• injection of activated, but not resting, human platelets enhanced rolling fraction to 48.9%, approaching a similar level to wild-type

increased T cell proliferation ( J:111233 )

• lymph node T cells from sensitized platelet-treated mutant mice proliferate comparably to wild-type in response to antigen while T cells from untreated sensitized mutants do not proliferate at all

cellular

abnormal leukocyte adhesion ( J:111233 )

• in untreated Sell-deficient mice, WBCs do not experience activation-dependent LFA-1 mediated arrest and adhere to high endothelial venules (HEV)

• infusion of activated platelets restores rolling capability in Sell-deficient WBCs and greater than 50% become stably adherent to HEV

impaired leukocyte tethering or rolling ( J:111233 )

• fraction of rolling Sell-deficient lymphocytes (12.1%) is reduced compared to wild-type littermates (72.1%)

• injection of activated, but not resting, human platelets enhanced rolling fraction to 48.9%, approaching a similar level to wild-type

increased T cell proliferation ( J:111233 )

• lymph node T cells from sensitized platelet-treated mutant mice proliferate comparably to wild-type in response to antigen while T cells from untreated sensitized mutants do not proliferate at all

Genotype
MGI:3664423

hm2

• Allelic Composition: Sell^tm1Pest/Sell^tm1Pest
• Genetic Background: involves: 129S4/SvJae * C57BL/6

immune system

immune system phenotype ( J:112550 )

N • Sell^tm1Pest homozygotes show a normal acute response to irritants, including recruitment of nonspecific effector cells such as macrophages and granulocytes

N • transfer of wild-type T lymphocytes into Sell-deficient mice restores the ability to respond to a contact hypersensitivity agent

enlarged spleen ( J:112550 )

• spleens of mutants are increased in size by more than 30%

abnormal splenic cell ratio ( J:112550 )

• mutants show splenic T cell numbers 25% greater than wild-type

lymph node hypoplasia ( J:112550 )

• lymphocyte numbers in mutant nodes are decreased almost 90% but percentages of CD4 and CD8 T cells, and B cells are similar to wild-type mice

small popliteal lymph nodes ( J:112550 )

• mice show a dramatic decrease in weights of popliteal lymph nodes (PLNs) compared to wild-type mice

abnormal cell-mediated immunity ( J:112550 )

• mice do not develop antigen-specific T cells in response to a contact hypersensitivity challenge

abnormal leukocyte migration ( J:112550 )

• in Sell-deficient mice, antigen-specific T cells are defective in homing to peripheral lymph nodes and subsequent activation in these nodes

impaired leukocyte tethering or rolling ( J:112550 )

• leukocytes do not exhibit rolling on peripheral node addressin (PNAd), the major ligand for L-Selectin on endothelial venules

decreased T cell proliferation ( J:112550 )

• after skine exposure to DNFB, lymph nodes examined 5 hours later after incubation with DNBS show 97% less T cell proliferation compared to wild-type lymph node T cells

• lymph node T cells proliferate comparably in vitro to wild-type controls in response to polyclonal activation with PMA + Ionomycin, concanavalin A or CD3 antibodies

• when dendritic cells are conjugated with DNBS and injected intravenously or subcutaneously, proliferation of T cells is substantial in splenic T cell cultures but proliferation in lymph node cultures is similar to background; in wild-type mice, T cell proliferation in splenic cultures occurs, but to a lesser extent than in lymph node cultures; results indicate that once response dependence on peripheral lymph nodes is removed, delayed type hypersensitization immune responses are intact

abnormal dendritic cell physiology ( J:112550 )

• antigen applied to epidermis of mutants is taken up by I-A+ dendritic cells which migrate in normal numbers to PLNs but not to other secondary lymphoid tissues

abnormal hypersensitivity reaction ( J:112550 )

hematopoietic system

abnormal leukocyte migration ( J:112550 )

• in Sell-deficient mice, antigen-specific T cells are defective in homing to peripheral lymph nodes and subsequent activation in these nodes

impaired leukocyte tethering or rolling ( J:112550 )

• leukocytes do not exhibit rolling on peripheral node addressin (PNAd), the major ligand for L-Selectin on endothelial venules

decreased T cell proliferation ( J:112550 )

• after skine exposure to DNFB, lymph nodes examined 5 hours later after incubation with DNBS show 97% less T cell proliferation compared to wild-type lymph node T cells

• lymph node T cells proliferate comparably in vitro to wild-type controls in response to polyclonal activation with PMA + Ionomycin, concanavalin A or CD3 antibodies

• when dendritic cells are conjugated with DNBS and injected intravenously or subcutaneously, proliferation of T cells is substantial in splenic T cell cultures but proliferation in lymph node cultures is similar to background; in wild-type mice, T cell proliferation in splenic cultures occurs, but to a lesser extent than in lymph node cultures; results indicate that once response dependence on peripheral lymph nodes is removed, delayed type hypersensitization immune responses are intact

enlarged spleen ( J:112550 )

• spleens of mutants are increased in size by more than 30%

abnormal splenic cell ratio ( J:112550 )

• mutants show splenic T cell numbers 25% greater than wild-type

cellular

abnormal leukocyte migration ( J:112550 )

• in Sell-deficient mice, antigen-specific T cells are defective in homing to peripheral lymph nodes and subsequent activation in these nodes

impaired leukocyte tethering or rolling ( J:112550 )

• leukocytes do not exhibit rolling on peripheral node addressin (PNAd), the major ligand for L-Selectin on endothelial venules

decreased T cell proliferation ( J:112550 )

• after skine exposure to DNFB, lymph nodes examined 5 hours later after incubation with DNBS show 97% less T cell proliferation compared to wild-type lymph node T cells

• lymph node T cells proliferate comparably in vitro to wild-type controls in response to polyclonal activation with PMA + Ionomycin, concanavalin A or CD3 antibodies

• when dendritic cells are conjugated with DNBS and injected intravenously or subcutaneously, proliferation of T cells is substantial in splenic T cell cultures but proliferation in lymph node cultures is similar to background; in wild-type mice, T cell proliferation in splenic cultures occurs, but to a lesser extent than in lymph node cultures; results indicate that once response dependence on peripheral lymph nodes is removed, delayed type hypersensitization immune responses are intact

growth/size/body

enlarged spleen ( J:112550 )

• spleens of mutants are increased in size by more than 30%

Genotype
MGI:3664424

ht3

• Allelic Composition: Sell^tm1Pest/Sell⁺
• Genetic Background: involves: 129S4/SvJae * C57BL/6

immune system

impaired leukocyte tethering or rolling ( J:112550 )

• leukocytes from heterozygotes show ~50% of wild-type levels of rolling along high endothelial venules (HEV)

abnormal hypersensitivity reaction ( J:112550 )

• response of sensitized mice to DFNB (contact hypersensitivity) is not significantly different from wild-type mice

cellular

impaired leukocyte tethering or rolling ( J:112550 )

• leukocytes from heterozygotes show ~50% of wild-type levels of rolling along high endothelial venules (HEV)

hematopoietic system

impaired leukocyte tethering or rolling ( J:112550 )

• leukocytes from heterozygotes show ~50% of wild-type levels of rolling along high endothelial venules (HEV)