Phenotypes associated with this allele

• Allele Symbol: Vtcn1^tm1Mak
• Allele Name: targeted mutation 1, Tak W Mak
• Allele ID: MGI:3663315
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Vtcn1^tm1Mak/Vtcn1^tm1Mak	C.Cg-Vtcn1^tm1Mak	MGI:3664250
hm2	Vtcn1^tm1Mak/Vtcn1^tm1Mak	involves: 129P2/OlaHsd * C57BL/6	MGI:3664249

Genotype
MGI:3664250

hm1

• Allelic Composition: Vtcn1^tm1Mak/Vtcn1^tm1Mak
• Genetic Background: C.Cg-Vtcn1^tm1Mak

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased interferon-gamma secretion ( J:112130 )

• upon restimulation in vitro, T cells produce ~2-fold higher levels of Ifng compared to wild-type BALB/c controls

decreased interleukin-4 secretion ( J:112130 )

• mutant T cells only show a marginal increase in production of Il-4 in the same culture

decreased susceptibility to parasitic infection ( J:112130 )

• mutants show lower responses when infected with Leishmania major compared to control BALB/c mice; mice display up to a 30% reduction in footpad swelling especially near the end of disease progression and reduced numbers of parasites in the infected footpad

Genotype
MGI:3664249

hm2

• Allelic Composition: Vtcn1^tm1Mak/Vtcn1^tm1Mak
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

normal phenotype

no abnormal phenotype detected ( J:112130 )

• homozygous mice do not develop spontaneous autoimmune disease; no immune cell infiltration indicative of autoimmune disease is observed in organs of homozygotes

• total cellularity and composition of immune cells in primary and secondary lymphoid organs is not different from controls

• levels of activated T cells in the periphery is not altered compared to wild-type

• basal serum levels of immunoglobulins are not different from wild-type

• inflammatory responses in models of hypersensitivity are unaltered

• antiviral cytotoxic T lymphocyte reactions are intact in mutants as shown by responses to LCMV and influenza virus A