Phenotypes associated with this allele

• Allele Symbol: Sp4^tm3(Sp4)Krc
• Allele Name: targeted mutation 3, Kenneth R Chien
• Allele ID: MGI:3663671
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sp4^tm3(Sp4)Krc/Sp4^tm3(Sp4)Krc	involves: 129S1/Sv * 129X1/SvJ * Black Swiss	MGI:3713111

Genotype
MGI:3713111

hm1

• Allelic Composition: Sp4^tm3(Sp4)Krc/Sp4^tm3(Sp4)Krc
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * Black Swiss

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal hippocampus morphology ( J:110135 )

• at 2 months, massive stable vacuoles are found in the grey matter in the CA3 region

• at 4 and 6 months, instable vacuoles are found in the infrapyramidal part of the hilus of dentate gyrus and stratum oriens in the ventral hippocampus

abnormal dentate gyrus morphology ( J:110135 )

• astrogliosis is found in the dentate gyrus

• however, no neural death is observed

gliosis ( J:110135 )

• astrogliosis is found in the dentate gyrus

• however, no neural death is observed

reduced sensorimotor gating ( J:110135 )

• mice show less inhibition of the startle response at each of the five prepulse intensities tested

reduced long-term potentiation ( J:163637 )

• long term potentiation is impaired in the hippocampal CA1 when a high-frequency train of stimuli is delivered to the afferent fibers

• however, paired-pulse facilitation and synaptic transmission are normal

behavior/neurological

impaired passive avoidance behavior ( J:110135 )

• shorter latency to enter the chamber that had been paired with a foot shock 24 hours previously

impaired contextual conditioning behavior ( J:110135 )

• mice show decreased freezing in the contextual fear conditioning test

impaired cued conditioning behavior ( J:110135 )

• mice show decreased freezing in the cued fear conditioning test

decreased exploration in new environment ( J:110135 )

• mice travel less distance in the center of an arena and the distance traveled in the center area as a function of total distance traveled was also reduced

impaired spatial learning ( J:163637 )

• in the Barnes maze, 6 month old mice make more errors, exhibit longer escape latencies and spend most of their time randomly searching for the target hole with little improvement in performance compared to controls during 12 consecutive sessions, indicating impaired spatial learning

• mice, however, exhibit normal head tracking in the optomotor test of vision suggesting normal visual ability

abnormal spatial reference memory ( J:163637 )

• in the Barnes maze, mice spend most of their time randomly searching for the target hole with little improvement in performance compared to controls during 12 consecutive sessions, indicating impaired spatial learning

• in the probe test of the Barnes maze, mice spend less time in the target quadrant than controls

abnormal startle reflex ( J:110135 )

• reduction in inhibition of startle response

impaired coordination ( J:110135 )

• mice show impairment in the cage top and wire hang tests

abnormal locomotor behavior ( J:110135 )

• less activity as measured by horizontal photobeam interruptions

decreased vertical activity ( J:110135 )

• less rearing

hyperactivity ( J:163637 )

• in the Y-maze test, mice are somewhat more active than wild-type mice, showing an increased number of entries, however spatial alternation is not impaired, suggesting that short-term memory is not affected

• mice however, exhibit similar locomotor activity as controls and no differences in anxiety-like behavior in the light/dark transfer test, or in object exploration or social interaction

cardiovascular system

cardiovascular system phenotype ( J:110135 )

N • unlike in null alleles no cardiac abnormalities are observed

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
schizophrenia		DOID:5419	OMIM:181500	J:163637