Phenotypes associated with this allele

• Allele Symbol: Fyb1^tm1Gak
• Allele Name: targeted mutation 1, Gary A Koretzky
• Allele ID: MGI:3663737
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Fyb1^tm1Gak/Fyb1^tm1Gak	B6.129-Fyb1^tm1Gak	MGI:3712970
hm2	Fyb1^tm1Gak/Fyb1^tm1Gak	involves: 129S1/Sv * 129X1/SvJ	MGI:3712982

Genotype
MGI:3712970

hm1

• Allelic Composition: Fyb1^tm1Gak/Fyb1^tm1Gak
• Genetic Background: B6.129-Fyb1^tm1Gak

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:112087 )

N • despite the expression of Fyb in NK cells, NK cell numbers and and functions are unaffected in homozygotes

Genotype
MGI:3712982

hm2

• Allelic Composition: Fyb1^tm1Gak/Fyb1^tm1Gak
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

immune system

abnormal leukocyte adhesion ( J:111368 )

• unlike in wild-type mice, there is no increase in adhesion following TCR stimulation

• however, treatement with phorbol 12-myristate 13-acetate (PMA) or Mn²⁺ does increase adhesion as it does in wild-type

decreased thymocyte number ( J:111368 )

• 188+/-66x10⁶ compared to 254+/-88x10⁶ in wild-type mice

abnormal T cell physiology ( J:126545 )

• in response to stimulation, T cells exhibit decreased aggregation compared to wild-type cells

• adhesion of stimulated T cells to ICAM1 is reduced compared to that of wild-type cells

• however, normal ICAM binding can be restored with high levels of stimulation of anti-CD3 antibodies

decreased T cell proliferation ( J:111368 , J:126545 )

• in response to anti-CD3epsilon (J:111368)

• proliferation of stimulated T cells is reduced compared to in wild-type mice (J:126545)

• however, normal proliferation can be restored with high levels of stimulation of anti-CD3 antibodies (J:126545)

decreased interleukin-2 secretion ( J:111368 , J:126545 )

• reduction in the production of IL-2 following anti-CD3epsilon stimulation (J:111368)

hematopoietic system

abnormal leukocyte adhesion ( J:111368 )

• unlike in wild-type mice, there is no increase in adhesion following TCR stimulation

• however, treatement with phorbol 12-myristate 13-acetate (PMA) or Mn²⁺ does increase adhesion as it does in wild-type

decreased thymocyte number ( J:111368 )

• 188+/-66x10⁶ compared to 254+/-88x10⁶ in wild-type mice

thrombocytopenia ( J:111368 )

• 410+/-74x10³ compared to 721+/-275x10³ in wild-type mice

abnormal T cell physiology ( J:126545 )

• in response to stimulation, T cells exhibit decreased aggregation compared to wild-type cells

• adhesion of stimulated T cells to ICAM1 is reduced compared to that of wild-type cells

• however, normal ICAM binding can be restored with high levels of stimulation of anti-CD3 antibodies

decreased T cell proliferation ( J:111368 , J:126545 )

• in response to anti-CD3epsilon (J:111368)

• proliferation of stimulated T cells is reduced compared to in wild-type mice (J:126545)

• however, normal proliferation can be restored with high levels of stimulation of anti-CD3 antibodies (J:126545)

cellular

abnormal leukocyte adhesion ( J:111368 )

• unlike in wild-type mice, there is no increase in adhesion following TCR stimulation

• however, treatement with phorbol 12-myristate 13-acetate (PMA) or Mn²⁺ does increase adhesion as it does in wild-type

decreased T cell proliferation ( J:111368 , J:126545 )

• in response to anti-CD3epsilon (J:111368)

• proliferation of stimulated T cells is reduced compared to in wild-type mice (J:126545)

• however, normal proliferation can be restored with high levels of stimulation of anti-CD3 antibodies (J:126545)

endocrine/exocrine glands

decreased thymocyte number ( J:111368 )

• 188+/-66x10⁶ compared to 254+/-88x10⁶ in wild-type mice