Phenotypes associated with this allele

• Allele Symbol: Hck^tm1Ern
• Allele Name: targeted mutation 1, Matthias Ernst
• Allele ID: MGI:3663799
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Hck^tm1Ern/Hck^tm1Ern	involves: 129S1/Sv * C57BL/6	MGI:3664860

Genotype
MGI:3664860

hm1

• Allelic Composition: Hck^tm1Ern/Hck^tm1Ern
• Genetic Background: involves: 129S1/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

increased circulating tumor necrosis factor level ( J:111432 )

• BALF from LPS-challenged mutants but not from challenged wild-type shows that a rapid and transient increase in levels of Tnfa (TNF alpha) occurs

abnormal enzyme/coenzyme activity ( J:111432 )

• an exaggerated induction of MMP-2 and -9 activity is found in BALF from LPS-challenged mutants; serine protease activity is also elevated

respiratory system

pulmonary hyperplasia ( J:111432 )

• lungs are consistently characterized by increased cellularity within the parenchyma

abnormal alveolar macrophage morphology ( J:111432 )

• there are large macrophages in mutant lungs despite specific pathogen-free (SPF) housing

• alveolar macrophages are frequently vacuolated with eosinophilic cytoplasm

lung inflammation ( J:111432 )

• lungs have areas of infiltration into the interstitium and alveoli at 3-4 months of age and older

• lungs are characterized by areas of accumulation of mononuclear inflammatory cells around airways and blood vessels

• areas of pulmonary consolidations become evident around 5-6 weeks of age and progress from that point

increased lung adenocarcinoma incidence ( J:111432 )

• in rare cases, pulmonary adenocarcinomas are detected in mice approaching 2 years of age

abnormal pulmonary alveolus morphology ( J:111432 )

• alveolar spaces contain eosinophilic matter accompanied by degranulated eosinophilic protein

atelectasis ( J:111432 )

• in 3-4 month-old and older mutants, lungs show areas of atelectasis

overexpanded pulmonary alveolus ( J:111432 )

• lungs show some areas of enlarged airspaces at 3-4 months of age and older

thick pulmonary interalveolar septum ( J:111432 )

• excessive fibrotic deposits of extracellular matrix in the alveolar septa

emphysema ( J:111432 )

• lungs show areas of emphysema, with loss of orderly appearance of acinus

pulmonary fibrosis ( J:111432 )

• lungs display signs of fibrosis characterized by depostion of extracellular matrix material in the alveolar septa

abnormal respiratory epithelium morphology ( J:111432 )

• epithelial cells lining the conducting airways exhibit extensive mucus cell metaplasia resulting in mucus aggregates in the airway lumen

respiratory epithelium hypertrophy ( J:111432 )

• epithelium lining conducting airways is frequently hypertrophic and many epithelial cells are enlarged due to excessive production/accumulation of mucus material

decreased lung elastance ( J:111432 )

• mutants show decreased tissue elastance of the lung tissue at baseline compared with wild-type

respiratory distress ( J:111432 )

• aging mice show signs of respiratory distress, evident by use of accessory muscles

hematopoietic system

increased eosinophil cell number ( J:111432 )

• eosinophils are most prominent cell type in infiltrates in lungs (30% of total bronchoalveolar lavage fluid (BALF) from mutants compared to 2% in wild-type

abnormal alveolar macrophage morphology ( J:111432 )

• there are large macrophages in mutant lungs despite specific pathogen-free (SPF) housing

• alveolar macrophages are frequently vacuolated with eosinophilic cytoplasm

abnormal macrophage physiology ( J:111432 )

• engulfed crystalline eosinophilic material is often observed in macrophages in mutant lungs

immune system

increased eosinophil cell number ( J:111432 )

• eosinophils are most prominent cell type in infiltrates in lungs (30% of total bronchoalveolar lavage fluid (BALF) from mutants compared to 2% in wild-type

abnormal alveolar macrophage morphology ( J:111432 )

• there are large macrophages in mutant lungs despite specific pathogen-free (SPF) housing

• alveolar macrophages are frequently vacuolated with eosinophilic cytoplasm

abnormal immune system physiology ( J:111432 )

• mutants have enhanced pulmonary innate immune response to LPS challenge

• extent of neutrophilic accumulation in BAL-F of LPS-challenged wild-type mice is 3-fold higher than mutants but number of eosinophils and macrophages (~1/3 of total cells) in BALF from unchallenged mutants are profoundly elevated compared to unchallenged wild-type

abnormal macrophage physiology ( J:111432 )

• engulfed crystalline eosinophilic material is often observed in macrophages in mutant lungs

increased circulating tumor necrosis factor level ( J:111432 )

• BALF from LPS-challenged mutants but not from challenged wild-type shows that a rapid and transient increase in levels of Tnfa (TNF alpha) occurs

increased interleukin-5 secretion ( J:111432 )

• BALF from mutant lungs have elevated levels of IL-5 compared to wild-type (70 pg/ml vs 17 pg/ml)

lung inflammation ( J:111432 )

• lungs have areas of infiltration into the interstitium and alveoli at 3-4 months of age and older

• lungs are characterized by areas of accumulation of mononuclear inflammatory cells around airways and blood vessels

• areas of pulmonary consolidations become evident around 5-6 weeks of age and progress from that point

neoplasm

increased lung adenocarcinoma incidence ( J:111432 )

• in rare cases, pulmonary adenocarcinomas are detected in mice approaching 2 years of age

growth/size/body

pulmonary hyperplasia ( J:111432 )

• lungs are consistently characterized by increased cellularity within the parenchyma