Analysis Tools
immune system
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• humoral response to T cell-dependent and thymus independent type 1 antigens is normal
• B cell proliferative responses are normal in culture
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• the increase in CD38 positive cells in the R2 fromction containing pre-B and immature B cells seen in wild-type is not observed in mutants
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• 2- to 3-week old Bst1-deficient mice have a 30-70% reduced number of peritoneal B-1 (CD5+) cells compared to controls; numbers recover to normal in mice older than 6 weeks
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• a minor B-lineage population in bone marrow and spleen that expresses low to no CD38 is increased in mutants (CD38low/- level - 1.5% and 0.6% in bone marrow and spleen respectively in wild-type vs 8.9 and 3.5% in mutants)
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• mucosal immune response to cholera toxin is impaired; levels of anti-CT IgA and IgG antibodies in fecal extracts are 2-fold lower than in wild-type littermates but sera levels are normal
• numbers of CT-specific IgA antibody forming cells (AFCs) are low in intestinal lamina propria but number of IgA and IgG AFCs are normal in the spleen and in mesenteric lymph nodes and Peyer's patches
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• levels of anti-CT IgA in fecal extracts of challenged mice are 2-fold lower than in wild-type
• number of CT-specific IgA AFCs is low in lamina propria, a major mucosal effector site for IgA production
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• when mice are challenged with thymus-independent type 2 antigen (TNP-Ficoll), a reduction in TNP-specific IgG3 is observed
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• when mice are challenged with thymus-independent type 2 antigen (TNP-Ficoll), a reduction in TNP-specific IgG3 is observed, but IgM production is normal
• levels of anti-CT IgA and IgG are 2-fold lower in fecal extracts after cholera toxin challenge
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hematopoietic system
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• the increase in CD38 positive cells in the R2 fromction containing pre-B and immature B cells seen in wild-type is not observed in mutants
|
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• 2- to 3-week old Bst1-deficient mice have a 30-70% reduced number of peritoneal B-1 (CD5+) cells compared to controls; numbers recover to normal in mice older than 6 weeks
|
|
• a minor B-lineage population in bone marrow and spleen that expresses low to no CD38 is increased in mutants (CD38low/- level - 1.5% and 0.6% in bone marrow and spleen respectively in wild-type vs 8.9 and 3.5% in mutants)
|
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• mucosal immune response to cholera toxin is impaired; levels of anti-CT IgA and IgG antibodies in fecal extracts are 2-fold lower than in wild-type littermates but sera levels are normal
• numbers of CT-specific IgA antibody forming cells (AFCs) are low in intestinal lamina propria but number of IgA and IgG AFCs are normal in the spleen and in mesenteric lymph nodes and Peyer's patches
|
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• levels of anti-CT IgA in fecal extracts of challenged mice are 2-fold lower than in wild-type
• number of CT-specific IgA AFCs is low in lamina propria, a major mucosal effector site for IgA production
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|
• when mice are challenged with thymus-independent type 2 antigen (TNP-Ficoll), a reduction in TNP-specific IgG3 is observed
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